公开(公告)号：US20210031144A1

公开(公告)日：2021-02-04

申请号：US17073300

申请日：2020-10-17

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Matthew Estes ,  Bruce Chabansky ,  Andrew Garst ,  Richard Fox ,  Jorge Bernate

IPC: B01D63/08

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200347342A1

公开(公告)日：2020-11-05

申请号：US16930269

申请日：2020-07-15

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200248131A1

公开(公告)日：2020-08-06

申请号：US16820324

申请日：2020-03-16

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200087613A1

公开(公告)日：2020-03-19

申请号：US16693630

申请日：2019-11-25

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky ,  Jorge Bernate ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200078738A1

公开(公告)日：2020-03-12

申请号：US16687640

申请日：2019-11-18

Applicant: Inscripta, Inc.

Inventor： Don Masquelier ,  Phillip Belgrader ,  Andrew Garst ,  Richard Fox ,  Matthew Estes ,  Bruce Chabansky

IPC: B01D63/08

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200063088A1

公开(公告)日：2020-02-27

申请号：US16597826

申请日：2019-10-09

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US11685889B2

公开(公告)日：2023-06-27

申请号：US17734037

申请日：2022-04-30

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky ,  Michael Graige

IPC: C12N15/00 ,  C12M3/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

CPC classification number: C12M35/08 ,  C12M23/44 ,  C12M33/14 ,  C12N15/11 ,  C12N15/902 ,  C12N2310/20

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US11332850B2

公开(公告)日：2022-05-17

申请号：US17554373

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C40B20/04 ,  C07K16/28 ,  C40B40/02 ,  C12N15/90 ,  C12N15/10 ,  C12N15/85 ,  C40B30/04 ,  C12N9/22 ,  C12N15/81 ,  C40B70/00 ,  C07K14/74 ,  C40B40/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20210363481A1

公开(公告)日：2021-11-25

申请号：US17319006

申请日：2021-05-12

Applicant: Inscripta, Inc.

Inventor： Eric Abbate ,  Katherine Krouse ,  Richard Fox ,  Daniel Held ,  Michael Clay ,  Nandini Krishnamurthy

IPC: C12N1/20 ,  C12N15/70

Abstract: The present disclosure relates to various different types of mutations in the thrA gene in E. coli leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US20210246574A1

公开(公告)日：2021-08-12

申请号：US17232046

申请日：2021-04-15

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.