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    Mammalian common lymphoid progenitor cell
    31.
    发明授权
    Mammalian common lymphoid progenitor cell 有权
    哺乳动物常见淋巴祖细胞

    公开(公告)号：US07297329B2

    公开(公告)日：2007-11-20

    申请号：US11093951

    申请日：2005-03-29

    申请人： Koichi Akashi ,  Irving L. Weissman ,  Motonari Kondo

    发明人： Koichi Akashi ,  Irving L. Weissman ,  Motonari Kondo

    IPC分类号： A01N65/00

    CPC分类号： C12N5/0635 ,  A61K38/00 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2510/00

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for lymphoid lineages, but lacking the potential to differentiate into myeloid and erythroid lineages. Methods are provided for the isolation and culture of this common lymphoid progenitor cell (CLP). The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor α); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. The murine cells are also characterized as expressing low levels of sca-1 (Ly-6E and Ly-6A). The CLPs are predominantly cycling, blast cells. These cells give rise to B cells, T cells and natural killer cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于淋巴谱系的祖细胞活性，但缺乏分化为骨髓和红系谱系的潜力。 提供了用于分离和培养这种常见淋巴祖细胞（CLP）的方法。 细胞富集方法使用特异性识别CDw127（IL-7受体α）的试剂; CD117（c-kit）蛋白，与在谱系确定细胞上表达的其他标志物结合。 鼠细胞也被表征为表达低水平的sca-1（Ly-6E和Ly-6A）。 CLP主要是循环，胚细胞。 这些细胞产生B细胞，T细胞和自然杀伤细胞，这通过其体外和体内的生长和分化证明。

    Comprehensive Methylome Map of Myeloid and Lymphoid Commitment from Hematopoietic Proenitors
    32.
    发明申请
    Comprehensive Methylome Map of Myeloid and Lymphoid Commitment from Hematopoietic Proenitors 审中-公开
    来自造血干细胞的骨髓和淋巴细胞承诺的综合甲基化图

    公开(公告)号：US20130281304A1

    公开(公告)日：2013-10-24

    申请号：US13814460

    申请日：2011-08-12

    申请人： Andrew P. Feinberg ,  Irving L. Weissman

    发明人： Andrew P. Feinberg ,  Irving L. Weissman

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6881 ,  C12Q2600/154

    摘要： Provided herein are differentially methylated regions (DMRs) of multipotent progenitor cells (MPPs) and oligopotent progenitor cells and methods of use thereof. The invention provides methods for detecting and analyzing alterations in the methylation status of DMRs in such progenitor cells as well as methods for differentiating such cells.

    摘要翻译： 本文提供了多能祖细胞（MPP）和寡能祖细胞的差异甲基化区域（DMR）及其使用方法。 本发明提供了用于检测和分析这些祖细胞中DMR的甲基化状态的变化的方法以及用于分化这些细胞的方法。

    Methods and Compositions for Treating Carcinoma Stem Cells
    34.
    发明申请
    Methods and Compositions for Treating Carcinoma Stem Cells 审中-公开
    治疗癌细胞干细胞的方法和组合

    公开(公告)号：US20110124032A1

    公开(公告)日：2011-05-26

    申请号：US12865092

    申请日：2009-01-30

    申请人： Maximilian Diehn ,  Michael Clarke ,  Irving L. Weissman

    发明人： Maximilian Diehn ,  Michael Clarke ,  Irving L. Weissman

    IPC分类号： C12Q1/02

    CPC分类号： G01N33/57496 ,  G01N33/5011 ,  G01N33/57407 ,  G01N33/57415 ,  G01N33/57419 ,  G01N2800/52

    摘要： Cancer stem cells (CSCs) have been prospectively isolated or identified from primary tumor samples, and shown to possess the unique properties of self-renewal and differentiation, and can form unique histological microdomains useful in cancer diagnosis. Such cancer stem cells are shown herein to have the phenotype of containing decreased levels of reactive oxygen species (ROS) relative to non-tumorigenic (non-stem cell) cancer cells, as well as expression of other protective pathways. The CSCs are further shown to be more resistant to ionizing radiation (IR) and certain chemotherapies and to express high levels of ROS genes.

    摘要翻译： 已经从原发性肿瘤样品前瞻性分离或鉴定了癌干细胞（CSCs），并显示其具有自我更新和分化的独特性质，并且可以形成用于癌症诊断的独特的组织学微区域。 这种癌干细胞在本文中显示为具有相对于非致瘤性（非干细胞）癌细胞降低的活性氧（ROS）水平以及其它保护途径的表达的表型。 进一步证明CSCs对电离辐射（IR）和某些化学疗法更具抗性，并表达高水平的ROS基因。

    WNT PATHWAY MUTATIONS IN CANCER STEM CELLS
    35.
    发明申请
    WNT PATHWAY MUTATIONS IN CANCER STEM CELLS 审中-公开
    癌症干细胞中的WNT途径突变

    公开(公告)号：US20100287631A1

    公开(公告)日：2010-11-11

    申请号：US12447884

    申请日：2007-11-02

    申请人： Catriona Helen M. Jamieson ,  Ifat Geron ,  Annelie Abrahamsson ,  Edward Kavalerchik ,  Irving L. Weissman ,  Jason Gotlib

    发明人： Catriona Helen M. Jamieson ,  Ifat Geron ,  Annelie Abrahamsson ,  Edward Kavalerchik ,  Irving L. Weissman ,  Jason Gotlib

    IPC分类号： C12Q1/48 ,  C12Q1/68 ,  G01N33/573 ,  A01K67/00

    CPC分类号： C12N9/1294 ,  A01K67/0271 ,  A01K2227/105 ,  A01K2267/0331 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136

    摘要： Cancer specific splicing events in the Wnt/β-catenin signaling pathway are associated with progression of myelogenous leukemia. Misspliced genes of interest include GSK3β. In some embodiments of the invention, polynucleotides are provided that correspond to misspliced GSK3β transcripts associated with cancer. Such transcripts are characterized by a deletion of exon (8), and particularly in exon (8) and (9). Detection of such transcripts in cells is indicative of the presence of leukemia, and particularly of the presence of leukemia stem cells. In other embodiments, polypeptides are provided that are encoded by misspliced GSK3β transcripts associated with cancer. Such polypeptides are useful as diagnostic markers for cancer, and as a target for screening of therapeutic agents. Animal models comprising a human LSC having a misspliced GSK3b transcript provide a useful model for leukemia, for drug/gene screening in the prevention and treatment of leukemia in humans, etc.

    摘要翻译： Wnt / bgr-catenin信号通路中的癌细胞特异性剪接事件与骨髓性白血病的进展有关。 感兴趣的未知基因包括GSK3＆bgr。 在本发明的一些实施方案中，提供了与错过的GSK3和bgr相对应的多核苷酸; 与癌症相关的成绩单。 这些转录物的特征在于外显子（8）的缺失，特别是在外显子（8）和（9）中。 细胞中这些转录物的检测表明存在白血病，特别是存在白血病干细胞。 在其他实施方案中，提供了由错过的GSK3和bgr编码的多肽; 与癌症相关的成绩单。 这样的多肽可用作癌症的诊断标记物，以及用作筛选治疗剂的靶标。 包含具有错误的GSK3b转录物的人LSC的动物模型为白血病提供了有用的模型，用于预防和治疗人类白血病等的药物/基因筛选。

    Identification and Isolation of Acute Myeloid Leukemia Stem Cells
    36.
    发明申请
    Identification and Isolation of Acute Myeloid Leukemia Stem Cells 有权
    鉴定和分离急性骨髓性白血病干细胞

    公开(公告)号：US20100255575A1

    公开(公告)日：2010-10-07

    申请号：US12817105

    申请日：2010-06-16

    申请人： Irving L. Weissman ,  Naoki Hosen

    发明人： Irving L. Weissman ,  Naoki Hosen

    IPC分类号： C12N5/09

    CPC分类号： G01N33/57426 ,  C12Q1/34 ,  G01N33/5011

    摘要： Acute myeloid leukemia stem cells (AMLSC) are identified. The cells can be prospectively isolated or identified from patient samples, and are shown to possess the unique properties of cancer stem cells in functional assays for cancer stem cell self-renewal and differentiation, and in cancer diagnosis.

    摘要翻译： 确定急性骨髓性白血病干细胞（AMLSC）。 可以从患者样品前瞻性分离或鉴定细胞，并且在癌症干细胞自我更新和分化以及癌症诊断的功能测定中显示具有癌干细胞的独特性质。

    Identification and isolation of acute myeloid leukemia stem cells
    39.
    发明申请
    Identification and isolation of acute myeloid leukemia stem cells 有权
    鉴定和分离急性骨髓性白血病干细胞

    公开(公告)号：US20080187950A1

    公开(公告)日：2008-08-07

    申请号：US11999809

    申请日：2007-12-07

    申请人： Irving L. Weissman ,  Naoki Hosen

    发明人： Irving L. Weissman ,  Naoki Hosen

    IPC分类号： C12Q1/02 ,  C12N5/00 ,  C12N5/08

    CPC分类号： G01N33/57426 ,  C12Q1/34 ,  G01N33/5011

    摘要： Acute myeloid leukemia stem cells (AMLSC) are identified. The cells can be prospectively isolated or identified from patient samples, and are shown to possess the unique properties of cancer stem cells in functional assays for cancer stem cell self-renewal and differentiation, and in cancer diagnosis.

    摘要翻译： 确定急性骨髓性白血病干细胞（AMLSC）。 可以从患者样品前瞻性分离或鉴定细胞，并且在癌症干细胞自我更新和分化以及癌症诊断的功能测定中显示具有癌干细胞的独特性质。

    MAMMALIAN MYELOID PROGENITOR CELL SUBSETS
    40.
    发明申请
    MAMMALIAN MYELOID PROGENITOR CELL SUBSETS 有权

    公开(公告)号：US20080131408A1

    公开(公告)日：2008-06-05

    申请号：US11874841

    申请日：2007-10-18

    申请人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    发明人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    IPC分类号： A01N63/00 ,  C12N5/00

    CPC分类号： C12N5/0647 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2503/00 ,  C12Q1/6881 ,  C12Q2600/158

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. Tηε χελλ ενιχημεντ μετηoδσ εμπλoψεαγεντσ τηατ σπεχαλλψεψoγνιζε Tηψ-1; ανδIΛ-7 Pα, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于骨髓谱系的祖细胞活性，但缺乏分化成淋巴谱系的潜力。 该群体进一步分为特定的骨髓祖细胞亚群，包括常见的髓系祖细胞（CMP），巨核细胞/红细胞祖细胞（MEP）和粒细胞/单核细胞谱系祖细胞（GMP）。 提供了用于分离和培养这些亚群体的方法。 CMP群体产生所有骨髓谱系，并且可以产生专门致力于红细胞/巨核细胞或骨髓单核细胞谱系的两个另外的和可分离的祖细胞群体。 “=”2.46mm“wi =”1.78mm“file =”US20080131408A1-20080605-P00001.TIF“img-content =”character“img-format =”tif“=”CUSTOM-CHARACTER-00001“ “/> iotachietamuepsilonnutau muepsilontauetaodeltasigma epsilonmupilambdaopsi epsilonalphagammaepsilonnutphaigma tauetaalphatau sigmapiepsilonchialphalambdalambdapsi epsilonpsiogammanuiotazetaepsilon Tetapsi-1; alphanudeltaILambda-7 Palpha，与在谱系确定的细胞上表达的其他标志物结合。 这些细胞产生各种骨髓细胞，包括巨核细胞，粒细胞，树突状细胞和红细胞，其体外和体内的生长和分化证明。