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50 条记录 (耗时 0.034 秒)

    Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis
    33.
    发明授权
    Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis 失效
    用于在枯草芽孢杆菌中克隆的异源基因产物的控制积累的方法和载体生物体

    公开(公告)号：US4952508A

    公开(公告)日：1990-08-28

    申请号：US64841

    申请日：1987-06-19

    申请人： Shing Chang ,  Hing C. Wong ,  Vaughan P. Wittman

    发明人： Shing Chang ,  Hing C. Wong ,  Vaughan P. Wittman

    IPC分类号： C12N1/21 ,  C12N9/86 ,  C12N15/62 ,  C12N15/75

    CPC分类号： C12N9/86 ,  C12N15/625 ,  C12N15/75 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/61 ,  C07K2319/75

    摘要： A method and a cloning vector are described for the controlled accumulation of cloned heterologous gene products in Bacillus subtilis. The cloning vector is capable of being replicated in B. subtilis and includes the heterologous gene located and oriented such as to be under the control of an operator, promoter, and ribosome binding site sequence. The gene codes for a protein which is under the control of a transport mechanism by which the protein is secreted by the B. subtilis. The gene product is recovered from the growth medium for the B. subtilis. The cloning vector is also capable of similar use in other bacteria such as E. coli.

    摘要翻译： 描述了克隆的异源基因产物在枯草芽孢杆菌中的受控积累的方法和克隆载体。 克隆载体能够在枯草芽孢杆菌中复制，并且包括位于和定向的异源基因，以便在操纵子，启动子和核糖体结合位点序列的控制下。 该基因编码一种蛋白质，其由蛋白质被枯草芽孢杆菌分泌的转运机制控制。 从枯草芽孢杆菌的生长培养基中回收基因产物。 克隆载体也能够在其他细菌如大肠杆菌中类似地使用。

    P53 binding T cell receptor molecules
    38.
    发明授权
    P53 binding T cell receptor molecules 有权
    P53结合T细胞受体分子

    公开(公告)号：US07456263B2

    公开(公告)日：2008-11-25

    申请号：US10163084

    申请日：2002-06-05

    申请人： Linda A. Sherman ,  Kimberlyn F. Card ,  Jon A. Weidanz ,  Hing C. Wong ,  Elizabeth L. Thomson

    发明人： Linda A. Sherman ,  Kimberlyn F. Card ,  Jon A. Weidanz ,  Hing C. Wong ,  Elizabeth L. Thomson

    IPC分类号： C07K16/30

    CPC分类号： C07K14/7051 ,  A61K38/00

    摘要： The invention provides T-cell receptor (TCR) molecules comprising a Vα chain and a Vβ chain that bind peptides derived from the p53 protein, preferably, the human p53 protein. The TCR molecules include both heterodimeric molecules and single chain molecules which specifically bind a sequence preferably spanning about amino acid positions 264-272 of the p53 protein displayed in the context of an HLA molecule, preferably, HLA-A2.1. Also disclosed are methods for making and using such TCR molecules. The invention has a wide spectrum of useful application including therapeutic uses and use in the detection of cells expressing p53 protein.

    摘要翻译： 本发明提供了包含Valpha链和结合来源于p53蛋白的肽优选人p53蛋白的Vbeta链的T细胞受体（TCR）分子。 TCR分子包括异源二聚体分子和单链分子，其特异性结合优选跨越在HLA分子，优选HLA-A2.1上下文中显示的p53蛋白的氨基酸位置264-272的序列。 还公开了制备和使用这种TCR分子的方法。 本发明具有广泛的有用应用，包括治疗用途和用于检测表达p53蛋白的细胞。

    Streptomyces promoter and method of use thereof
    40.
    发明授权
    Streptomyces promoter and method of use thereof 失效
    结构促进剂及其使用方法

    公开(公告)号：US5164305A

    公开(公告)日：1992-11-17

    申请号：US466981

    申请日：1990-01-18

    申请人： Hing C. Wong

    发明人： Hing C. Wong

    IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N15/76

    CPC分类号： C12N9/0069 ,  C12N15/76 ,  C12Y113/11001 ,  C12Y113/11002

    摘要： A DNA sequence is described, which is a promoter for Streptomyces. This promoter is stronger than the wild type and ultimately increases transcription initiation and protein expression. Typically, a nucleotide base, such as guanine, is inserted into the promoter sequence between positions -50 and -75 to increase transcription initiation or protein expression. In a preferred embodiment, guanine is inserted between positions -62 and -63 of the promoter regulated by thiostrepton (tipA).

    摘要翻译： 描述了DNA序列，其是链霉菌的启动子。 该启动子比野生型强，并最终增加转录起始和蛋白表达。 通常，将核苷酸碱基（例如鸟嘌呤）插入到位于-50和-75之间的启动子序列中以增加转录起始或蛋白质表达。 在优选的实施方案中，将鸟嘌呤插入由硫链丝氨肽（tipA）调节的启动子的-62和-63之间。