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82 条记录 (耗时 0.035 秒)

    Method for cloning and expression of BsmAi restriction endonuclease and BsmAI methylase in E. coli
    32.
    发明授权
    Method for cloning and expression of BsmAi restriction endonuclease and BsmAI methylase in E. coli 失效
    用于在大肠杆菌中克隆和表达BsmAi限制性内切核酸酶和BsmAI甲基化酶的方法

    公开(公告)号：US06596524B2

    公开(公告)日：2003-07-22

    申请号：US09957005

    申请日：2001-09-20

    申请人： Zhenyu Zhu ,  Jing Zhou ,  Shuang-yong Xu

    发明人： Zhenyu Zhu ,  Jing Zhou ,  Shuang-yong Xu

    IPC分类号： C12N922

    CPC分类号： C12N9/22 ,  C12N9/1007

    摘要： The present invention relates to recombinant DNA which encodes the BsmAI restriction endonuclease as well as BsmAI methylase, expression of BsmAI restriction endonuclease and BsmAI methylase in E. coli cells containing the recombinant DNA, and purification of BsmAI endonuclease to near homogeneity.

    摘要翻译： 本发明涉及编码BsmAI限制性内切核酸酶以及BsmAI甲基化酶的重组DNA，含有重组DNA的大肠杆菌细胞中BsmAI限制性内切核酸酶和BsmAI甲基化酶的表达，以及将BsmAI内切核酸酶纯化至接近同质性。

    Method for determining the nucleotide sequence of the gene for the &agr;5(IV) chain of human type IV collagen
    33.
    发明授权
    Method for determining the nucleotide sequence of the gene for the &agr;5(IV) chain of human type IV collagen 失效
    确定人IV型胶原的α5（IV）链的基因的核苷酸序列的方法

    公开(公告)号：US06576418B1

    公开(公告)日：2003-06-10

    申请号：US08692989

    申请日：1996-08-07

    申请人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Jing Zhou

    发明人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Jing Zhou

    IPC分类号： C12Q168

    CPC分类号： C07K14/78 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/156

    摘要： The present invention relates to a method for isolating and identifying the nucleotide sequence of the human gene for the type IV collagen &agr;5(IV) chain. The present invention is directed to the determination of the nucleotide sequence of the gene for the &agr;5(IV) collagen chain in individuals by any method known to the art, e.g. cloning from genomic DNA libraries or amplifying gene regions with the polymerase chain reaction (PCR) and studying their physical properties or nucleotide sequences. In addition, the invention is directed to the use of the nucleotide sequences of the &agr;5(IV) gene to amplify or identify the nucleotide sequences of the &agr;5(IV) gene.

    摘要翻译： 本发明涉及用于分离和鉴定IV型胶原α5（IV）链的人基因的核苷酸序列的方法。 本发明涉及通过本领域已知的任何方法测定个体中α5（IV）胶原链的基因的核苷酸序列，例如， 通过聚合酶链反应（PCR）从基因组DNA文库克隆或扩增基因区域，并研究其物理性质或核苷酸序列。 此外，本发明涉及α5（IV）基因的核苷酸序列用于扩增或鉴定α5（IV）基因的核苷酸序列的用途。

    Method for cloning and producing the BsmI restriction endonuclease in E. coli
    34.
    发明授权
    Method for cloning and producing the BsmI restriction endonuclease in E. coli 失效
    用于在大肠杆菌中克隆和产生BsmI限制性内切核酸酶的方法

    公开(公告)号：US06335190B1

    公开(公告)日：2002-01-01

    申请号：US09693147

    申请日：2000-10-20

    申请人： Jing Zhou ,  Zhenyu Zhu ,  Shuang-yong Xu

    发明人： Jing Zhou ,  Zhenyu Zhu ,  Shuang-yong Xu

    IPC分类号： C12N1555

    CPC分类号： C12N9/22 ,  C12N9/1007

    摘要： The present invention relates to recombinant DNA which encodes the BsmI restriction endonuclease as well as BsmI methyltransferases, expression of BsmI restriction endonuclease in E. coil cells containing the recombinant DNA by using a low copy number T7 expression vector pACYC-T7ter, and purification of BsmI restriction endonuclease by heat treatment and chromatography through heparin Sepharose column.

    摘要翻译： 本发明涉及编码BsmI限制性内切核酸酶以及BsmI甲基转移酶的重组DNA，通过使用低拷贝数T7表达载体pACYC-T7ter，含有重组DNA的大肠杆菌细胞中BsmI限制性内切核酸酶的表达，以及纯化BsmI 通过热处理和通过肝素琼脂糖凝胶柱色谱法进行限制性内切核酸酶。

    Radiation thermometer using off-focus telecentric optics
    36.
    发明授权
    Radiation thermometer using off-focus telecentric optics 有权
    辐射温度计使用离焦远心光学

    公开(公告)号：US09448119B2

    公开(公告)日：2016-09-20

    申请号：US13531162

    申请日：2012-06-22

    申请人： Guray Tas ,  Jing Zhou ,  Daewon Kwon

    发明人： Guray Tas ,  Jing Zhou ,  Daewon Kwon

    IPC分类号： G01J5/00 ,  G01J5/08 ,  G01J5/06 ,  C23C16/44

    CPC分类号： G01J5/0831 ,  C23C16/44 ,  G01J5/0007 ,  G01J5/06 ,  G01J5/0806 ,  G01J5/0859

    摘要： A radiation thermometer utilizing an off-focus telecentric lens arrangement in chemical vapor deposition reactors. An object assembly of one or more optical components is positioned at a distance equal to its focal length from an aperture stop. The aperture stop is dimensioned so that the chief rays are substantially parallel with the optical axis of the object assembly, and so that the rays that pass through the aperture stop define a narrow solid angle about the chief rays. The off-focus telecentric arrangement thus configured is focused at infinity, but is utilized to capture radiation from a relatively proximate target (e.g., within a couple meters) that is out of focus. The capture of collimated radiation from the target diminishes the contribution of stray radiation, particularly with targets having a highly specular surface.

    摘要翻译： 在化学气相沉积反应器中使用离焦远心透镜装置的辐射温度计。 一个或多个光学部件的物体组件被定位成等于其孔径光阑的焦距的距离。 孔径光阑的尺寸使得主光线基本上与物体组件的光轴平行，并且使得穿过孔径光阑的光线围绕主光线限定窄的立体角。 如此构造的离焦远心布置被聚焦在无限远处，但是被用于从不相关的相对近的目标（例如，在几米内）捕获辐射。 来自目标的准直辐射的捕获减少了杂散辐射的贡献，特别是具有高度镜面的目标。

    Motif-specific and context-independent antibodies that specifically bind to a cleaved caspase motif
    38.
    发明授权
    Motif-specific and context-independent antibodies that specifically bind to a cleaved caspase motif 有权
    与特异性结合切割的半胱天冬酶基序的母体特异性和上下文相关抗体

    公开(公告)号：US09249231B2

    公开(公告)日：2016-02-02

    申请号：US13289651

    申请日：2011-11-04

    申请人： Michael Comb ,  Ailan Guo ,  John Edward Rush, II ,  Jun-Ming Cai ,  Jing Li ,  Jing Zhou

    发明人： Michael Comb ,  Ailan Guo ,  John Edward Rush, II ,  Jun-Ming Cai ,  Jing Li ,  Jing Zhou

    IPC分类号： C07K16/44 ,  C07K16/00 ,  C12N5/07

    CPC分类号： C07K16/18 ,  C07K16/44 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/33 ,  C07K2317/34

    摘要： There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.

    摘要翻译： 提供了一种特异性基因，独立于上下文的抗体，其特异性结合由（i）至少一个sumoylated赖氨酸残基组成的复发性修饰基序，和（ii）通过肽键与所述sumoylated结合的一个或多个简并氨基酸 赖氨酸残基，所述抗体特异性结合所述基序在其再次发生的生物体内的多个非同源肽或蛋白质中。 还提供了一种特异性基因，独立于上下文的抗体，其特异性结合由（i）C末端天冬氨酸残基组成的重复修饰基序，和（ii）一个或多个与肽键结合的简并氨基酸与所述 C末端天冬氨酸残基，所述抗体特异性结合所述基序在其再次发生的生物体内的多个非同源肽或蛋白质中。

    Verifying correctness of a database system via extended access paths
    39.
    发明授权
    Verifying correctness of a database system via extended access paths 有权
    通过扩展访问路径验证数据库系统的正确性

    公开(公告)号：US09195730B2

    公开(公告)日：2015-11-24

    申请号：US13252447

    申请日：2011-10-04

    申请人： Dong Sheng Chen ,  Yan Jia ,  Shu Hua Liu ,  Ke Wei Wei ,  Xin Ying Yang ,  Jing Zhou ,  Xiang Zhou

    发明人： Dong Sheng Chen ,  Yan Jia ,  Shu Hua Liu ,  Ke Wei Wei ,  Xin Ying Yang ,  Jing Zhou ,  Xiang Zhou

    IPC分类号： G06F7/00 ,  G06F17/30

    CPC分类号： G06F17/30595

    摘要： The invention provides for verifying correctness of a database system. A SQL instruction is received and access paths of the received SQL instruction are extended. The SQL instruction is executed by using the extended access paths, and the correctness of the database system is verified according to results of executing the SQL instruction. With the method and system of the invention, the object of verifying correctness of a database system by automatically extending access paths of SQL statement may be achieved, and the object of verifying correctness of a database system scientifically, effectively and purposefully based on ratio of error or defect present in database itself due to various data manipulation approaches (different values of access path elements) may also be achieved.

    摘要翻译： 本发明提供了验证数据库系统的正确性。 接收到SQL指令，扩展接收的SQL指令的访问路径。 通过使用扩展访问路径执行SQL指令，并根据执行SQL指令的结果验证数据库系统的正确性。 利用本发明的方法和系统，可以实现通过自动扩展SQL语句的访问路径来验证数据库系统的正确性的目的，并且基于错误比率来科学，有效和有目的地验证数据库系统的正确性的目的 或者由于各种数据操作方法（不同的访问路径元素值）而存在于数据库本身中的缺陷也可以实现。