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    Polypeptides with lipase activity and polynucleotides encoding same
    36.
    发明授权
    Polypeptides with lipase activity and polynucleotides encoding same 有权

    公开(公告)号：US09631164B2

    公开(公告)日：2017-04-25

    申请号：US14770006

    申请日：2014-03-19

    申请人： Novozymes A/S

    发明人： Marc Dominique Morant ,  Anna Verana Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

    IPC分类号： C12N9/20 ,  C12Q1/34 ,  C12Q1/44 ,  C07H21/04 ,  C11D3/00 ,  C11D7/44 ,  C12S11/00 ,  C11D3/386 ,  C12P13/00

    CPC分类号： C11D3/38627 ,  C11D3/38681 ,  C12N9/20 ,  C12P13/008

    摘要： The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3 or the full-length complement of (i); (c) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3; (d) a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and (e) a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

    Polypeptides with Lipase Activity And Polynucleotides Encoding Same
    37.
    发明申请
    Polypeptides with Lipase Activity And Polynucleotides Encoding Same 审中-公开
    具有脂肪酶活性的多肽和编码相同的多核苷酸

    公开(公告)号：US20160160196A1

    公开(公告)日：2016-06-09

    申请号：US14902679

    申请日：2014-07-08

    申请人： NOVOZYMES A/S

    发明人： Marc Dominique Morant ,  Anna Verena Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

    IPC分类号： C12N9/20 ,  C11D3/386

    CPC分类号： C12N9/20 ,  C11D3/38627 ,  C11D3/38681 ,  C12Y301/01 ,  C12Y301/01003 ,  D06L4/40

    摘要： The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 1 or the full-length complement of (i); (c)a polypeptide encoded by a polynucleotide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 1; (d) a polypeptide which is a variant of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and(e)a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

    摘要翻译： 本发明涉及具有脂肪酶活性的分离的多肽，其选自：（a）多肽，其具有至少80％，至少85％，至少90％，至少91％，至少92％，在 与SEQ ID NO：2的至少93％，至少94％，至少95％，至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （b）由多核苷酸编码的多肽，其在低严格条件，中等严格条件，中等严格条件，高严格条件或非常高严格条件下与（i）SEQ ID NO：1或全长补体 的（i）; （c）由多核苷酸编码的多肽，所述多肽具有至少80％，至少85％，至少90％，至少91％，至少92％，至少93％，至少94％，至少95％ 与SEQ ID NO：1的至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （d）在一个或多个（例如多个）位置上包含取代，缺失和/或插入的SEQ ID NO：2的变体的多肽; 和（e）作为（a），（b），（c）或（d）的多肽的片段的多肽。 本发明还涉及编码多肽的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。