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公开(公告)号：US08999685B2
公开(公告)日：2015-04-07
申请号：US14269591
申请日：2014-05-05
Applicant: Novozymes, Inc.
Inventor： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
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公开(公告)号：US20140234925A1
公开(公告)日：2014-08-21
申请号：US14269591
申请日：2014-05-05
Applicant: Novozymes, Inc.
Inventor： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
IPC: C12P7/46
Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
Abstract translation: 本发明涉及生产C4二羧酸的方法，其包括：（a）培养丝状真菌宿主细胞，所述丝状真菌宿主细胞包含选自以下的多核苷酸：编码C4二羧酸转运蛋白的异源第一多核苷酸，编码 苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中当在相同条件下培养时，丝状真菌宿主细胞能够分泌与不含异源多核苷酸的丝状真菌宿主细胞相比增加的C4二羧酸水平; 和（b）回收C4二羧酸。 本发明还涉及增加C4二羧酸生产，丝状真菌宿主细胞和苹果酸脱氢酶变体的方法。
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Patent Agency Ranking
33.发明申请Polypeptides Having Alpha-Glucosidase Activity And Polynucleotides Encoding Same 审中-公开具有α-葡糖苷酶活性的多肽和编码相同的多核苷酸公开(公告)号：US20140227391A1
公开(公告)日：2014-08-14
申请号：US14104756
申请日：2013-12-12
Applicant: NOVOZYMES, INC.
Inventor： Suzanne Otani , Haiyan Ge , Paul Harris , Debbie Yaver , Alexander Blinkovsky
CPC classification number: C12N9/2408 , C12C5/004 , C12C7/04 , C12C7/20 , C12C11/00 , C12C12/02 , C12N9/2411 , C12N15/8245 , C12Y302/0102
Abstract: The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Abstract translation: 本发明涉及具有α-葡糖苷酶活性的分离多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。
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34.发明申请公开(公告)号：US20140142278A1
公开(公告)日：2014-05-22
申请号：US14081340
申请日：2013-11-15
Applicant: Novozymes, Inc. , Novozymes A/S
Inventor： Mads Bjornvad , Debbie Yaver
IPC: C07K14/37
CPC classification number: C12P21/02 , C07K14/37 , C07K2319/02 , C12N9/20 , C12N15/625 , C12N15/81 , C12Y301/01003
Abstract: The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.
Abstract translation: 本发明涉及生产具有生物活性的多肽的方法，其包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含编码多肽的第一多核苷酸，其可操作地连接到 编码变体信号肽或变体前肽的第二个多核苷酸; 和（b）从培养基中分离具有生物活性的分泌多肽。
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公开(公告)号：US08563286B2
公开(公告)日：2013-10-22
申请号：US13762135
申请日：2013-02-07
Applicant: Novozymes, Inc. , Novozymes A/S
Inventor： Debbie Yaver , Janine Lin , Michael Lamsa , Kim Borch , Shamkant Anant Patkar
CPC classification number: C12N9/20
Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
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公开(公告)号：US20130152234A1
公开(公告)日：2013-06-13
申请号：US13762135
申请日：2013-02-07
Applicant: Novozymes, Inc. , Novozymes A/S
Inventor： Debbie Yaver , Janine Lin , Michael Lamsa , Kim Borch , Shamkant Anant Patkar
IPC: C12N9/20
CPC classification number: C12N9/20
Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
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37.发明申请公开(公告)号：US20130123163A1
公开(公告)日：2013-05-16
申请号：US13747184
申请日：2013-01-22
Applicant: Novozymes, Inc. , Novozymes A/S
Inventor： Suzanne Otani , Debbie Yaver , Haiyan Ge , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC: C12N9/20
CPC classification number: C12N9/20 , C12Y301/01003
Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Abstract translation: 本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。
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