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40 条记录 (耗时 0.041 秒)

    Process for producing CIS-3-hydroxy-L-proline
    31.
    发明授权
    Process for producing CIS-3-hydroxy-L-proline 有权
    制备CIS-3-羟基-L-脯氨酸的方法

    公开(公告)号：US06413748B1

    公开(公告)日：2002-07-02

    申请号：US09455406

    申请日：1999-12-06

    申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

    发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

    IPC分类号： C12P1324

    CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

    摘要： An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.

    摘要翻译： 用于生产顺式-3-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于链霉菌属或芽孢杆菌属的微生物的酶源存在下转化为顺式-3-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化为顺式 - 3-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，在水性介质中收集生成的顺式-3-羟基-L-脯氨酸。 一种新的L-脯氨酸-3-羟化酶，可用于该方法的L-脯氨酸-3-羟化酶的基因，含该基因的转化体，以及使用转化体产生L-脯氨酸-3-羟化酶的方法。

    Process for producing trans-4-hydroxy-L-proline
    32.
    发明授权
    Process for producing trans-4-hydroxy-L-proline 失效
    制备反式-4-羟基-L-脯氨酸的方法

    公开(公告)号：US06242231B1

    公开(公告)日：2001-06-05

    申请号：US09104382

    申请日：1998-06-25

    申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

    发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

    IPC分类号： C12N902

    CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

    摘要： An industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase which is useful for the process, a gene of L-proline-4-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-4-hydroxylase using the transformant. In addition, provided is a process for producing L-proline-4-hydroxylase using the transformant which contains the gene and has a reinforced proline biosynthesis activity.

    摘要翻译： 用于生产反式-4-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品的添加剂。 在此过程中，L-脯氨酸在衍生自属于Dactylosporangium，Amycolatopsis或Streptomyces属的微生物的酶源的存在下转化成反式-4-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化 在水性介质中转化成反式-4-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，从水性介质中收集生成的反式-4-羟基-L-脯氨酸。 还提供了可用于该方法的新型L-脯氨酸-4-羟化酶，可用于该方法的L-脯氨酸-4-羟化酶的基因，含有该基因的转化体的基因， 脯氨酸-4-羟化酶。 此外，提供了使用含有该基因并具有增强的脯氨酸生物合成活性的转化体生产L-脯氨酸-4-羟化酶的方法。

    Method for quantitative determination of 1,5-anhydroglucitol
    33.
    发明授权
    Method for quantitative determination of 1,5-anhydroglucitol 失效
    1,5-脱水葡萄糖醇定量测定方法

    公开(公告)号：US6153419A

    公开(公告)日：2000-11-28

    申请号：US930709

    申请日：1997-10-16

    申请人： Kazuo Aisaka ,  Sakae Tazoe ,  Katsuhiko Ando ,  Keiko Ochiai

    发明人： Kazuo Aisaka ,  Sakae Tazoe ,  Katsuhiko Ando ,  Keiko Ochiai

    IPC分类号： C12N9/24 ,  C12Q1/34 ,  C12Q1/48

    CPC分类号： C12Q1/34 ,  C12N9/2402 ,  C12Q1/48 ,  C12Y302/01028 ,  G01N2333/355 ,  G01N2333/924 ,  Y10S435/872

    摘要： The present invention relates to a method for quantitative determination of 1,5-anhydroglucitol in a sample, which comprises mixing the sample and an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, and measuring the activity of the enzyme; and a reagent for quantitative determination of 1,5-anhydroglucitol which comprises an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, a substrate for the enzyme, and a reagent for quantitative determination of a product formed by the enzyme activity. The present invention also relates to novel trehalase having a Ki value of 0.33 mM or less for 1,5-anhydroglucitol; and a process for producing novel trehalase having the above-mentioned physicochemical properties, which comprises culturing in a medium a microorganism belonging to the genus Nocardia and capable of producing the trehalase, and recovering the trehalase from the culture.

    摘要翻译： PCT No.PCT / JP97 / 00440 Sec。 371日期：1997年10月16日 102（e）1997年10月16日PCT PCT 1997年2月19日PCT公布。 公开号WO97 / 31103 日期：1997年8月28日本发明涉及一种样品中1,5-脱水葡萄糖醇的定量测定方法，该方法包括以浓度依赖性方式将样品与具有1,5-脱水葡萄糖醇抑制活性的酶 ，并测定酶的活性; 以及用于定量测定1,5-脱水葡萄糖醇的试剂，该试剂含有以浓度依赖性方式被1,5-脱水葡萄糖醇抑制的活性的酶，酶底物和定量产物形成试剂 通过酶活性。 本发明还涉及1,5-脱水葡萄糖醇的Ki值为0.33mM以下的新型海藻糖酶; 以及具有上述物理化学性质的新型海藻糖酶的制造方法，其包括在培养基中培养属于诺卡氏菌属的微生物并能够生产海藻糖酶，并从培养物中回收海藻糖酶的方法。

    Method of and apparatus for low-pressure casting of light metal alloy
    39.
    发明授权
    Method of and apparatus for low-pressure casting of light metal alloy 失效
    轻金属合金低压铸造方法及装置

    公开(公告)号：US4875518A

    公开(公告)日：1989-10-24

    申请号：US233144

    申请日：1988-08-17

    申请人： Takeshi Imura ,  Katsuhiko Ando ,  Shigemitsu Nakabayashi ,  Maki Hirose

    发明人： Takeshi Imura ,  Katsuhiko Ando ,  Shigemitsu Nakabayashi ,  Maki Hirose

    IPC分类号： B22C9/06 ,  B22D15/00 ,  B22D18/04 ,  B22D27/04

    CPC分类号： B22D18/04 ,  B22C9/061 ,  B22D15/005 ,  B22D27/04

    摘要： A light metal alloy such as an aluminum alloy is cast into a casting such as an automobile engine cylinder head under low pressure. A fluid pressure is applied to the surface of a light metal alloy stored in a closed container to introduce the light metal alloy from the closed container through a transfer tube and a sprue into a mold cavity defined in a mold assembly. The introduced molten metal alloy is brought into contact with molds of the mold assembly which have different thermal conductivities, respectively. The time in which the molten metal alloy is solidified is controlled by the molds of different thermal conductivities. The molds may forcibly be cooled by a cooling medium passing therethrough or cooling blocks mounted thereon.

    摘要翻译： 轻质金属合金如铝合金在低压下铸造成诸如汽车发动机气缸盖的铸件。 将流体压力施加到存储在密闭容器中的轻金属合金的表面，以将来自密闭容器的轻金属合金通过输送管和浇道引入模具组件中限定的模腔中。 引入的熔融金属合金分别与具有不同热导率的模具组件的模具接触。 熔融金属合金固化的时间由不同导热系数的模具控制。 模具可以通过穿过的冷却介质或安装在其上的冷却块强制地冷却。