公开(公告)号：US5876117A

公开(公告)日：1999-03-02

申请号：US964406

申请日：1997-11-04

申请人： Yin Chen

发明人： Yin Chen

IPC分类号： B01F7/00 ,  B01F7/02 ,  B01F7/08 ,  B01F13/00

CPC分类号： B01F7/027 ,  B01F7/00025 ,  B01F7/088 ,  B01F13/0035 ,  B01F7/00425

摘要： An agitator includes a trough having a first end, a second end, a top, a bottom, an inlet on the top and close to the second end, and an outlet on the bottom and close to the first end, an axle rotatably mounted between the first and second ends of the trough and enclosed with an inner spiral vane, the inner spiral vane being fixedly mounted on the axle and gradually decreased in outer diameter, the inner spiral vane being provided with an edge close to the first end of the trough, and an outer spiral vane enclosing the inner spiral vane and fixedly mounted on the inner spiral vane, the outer spiral vane being opposite to the inner spiral vane in spiral direction, whereby the agitator can be stir a mixture thoroughly and rapidly.

摘要翻译： 搅拌器包括槽，该槽具有第一端，第二端，顶部，底部，顶部和接近第二端的入口，以及位于底部并靠近第一端的出口，可转动地安装在 槽的第一端和第二端被内螺旋叶片封闭，内螺旋叶片固定地安装在轴上并且外径逐渐减小，内螺旋叶片设置有靠近槽的第一端的边缘 以及包围内螺旋叶片并固定地安装在内螺旋叶片上的外螺旋叶片，外螺旋叶片在螺旋方向与内螺旋叶片相对，从而搅拌器可以彻底快速地搅拌混合物。

公开(公告)号：US08889397B2

公开(公告)日：2014-11-18

申请号：US10574254

申请日：2004-06-03

申请人： Yin Chen ,  Xin Xing Tan

发明人： Yin Chen ,  Xin Xing Tan

IPC分类号： C12N15/74 ,  C12N7/00 ,  C12N1/20 ,  C12N1/00 ,  C12Q1/68 ,  A61K48/00 ,  C12N15/113 ,  C12N15/10 ,  C12N15/11

CPC分类号： C12N15/113 ,  C07H21/04 ,  C12N15/1034 ,  C12N15/111 ,  C12N15/74 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/127 ,  C12N2320/11 ,  C12N2330/31 ,  C12Q1/6837 ,  C12Q2565/531

摘要： A selectively inducible, single-stranded DNA (ssDNA) expression library, a method for constructing a ssDNA expression library, a method for screening ssDNA using the expression library, and a method for identifying ssDNA molecules that alter expression of bacterial and fungal gene(s) related to cell growth and toxin production and secretion. The screening library is used to, among other things, identify ODNs effective in stopping cell growth, killing bacteria or fungi, or preventing bacteria and/or fungi from synthesizing and secreting their toxins, and/or to discover ODNs effective in eukaryotic (e.g., mammalian) cells for targeted alteration of gene function. The library is also useful for identifying ssDNAs or ODNs that are used as therapeutic agents for, for instance, providing a method for treatment of bacterial infections such as sepsis.

摘要翻译： 选择性诱导型单链DNA（ssDNA）表达文库，构建ssDNA表达文库的方法，使用表达文库筛选ssDNA的方法以及鉴定改变细菌和真菌基因表达的ssDNA分子的方法 ）与细胞生长和毒素生成和分泌有关。 除了别的以外，筛选文库用于鉴别有效阻止细胞生长，杀死细菌或真菌，或阻止细菌和/或真菌合成和分泌其毒素的ODN，和/或发现在真核生物中有效的ODN（例如， 哺乳动物）细胞用于靶向改变基因功能。 该文库也可用于鉴定用作治疗剂的ssDNA或ODN，例如提供治疗细菌感染如败血症的方法。

公开(公告)号：US20140009074A1

公开(公告)日：2014-01-09

申请号：US13993682

申请日：2010-12-13

申请人： Yin Chen

发明人： Yin Chen

IPC分类号： F21S4/00

CPC分类号： F21S4/10 ,  F21S4/15 ,  F21V23/001 ,  F21Y2115/10 ,  H01L2224/45144 ,  H01L2224/48091 ,  H01L2924/00014 ,  H01L2924/00

摘要： The present invention relates to LED lamp string, comprising at least three conducting wires with insulating layers. One of the conducting wires is a positive conducting wire, and the others are negative conducting wires. A mounting part is provided on each negative conducting wire at every certain interval. A light emitting wafer is fixed to the mounting part. The light emitting wafer comprises a first electrode and a second electrode which are separately provided. The first electrode of the light emitting wafer connected with each negative conducting wire is electrically connected with the conductor of the same negative conducting wire. The second electrode is electrically connected with the conductor of the positive conducting wire via a wire. A transparent outer sealing gum encapsulating the mounting part, the light emitting wafer and the wire is provided on the LED lamp string.

摘要翻译： 本发明涉及LED灯串，其包括至少三根具有绝缘层的导线。 其中一条导线是正导线，其他导线是负导线。 每隔一定间隔在每条负导线上设置安装部件。 发光晶片固定在安装部分上。 发光晶片包括单独设置的第一电极和第二电极。 与每个负导线连接的发光晶片的第一电极与相同的负导线的导体电连接。 第二电极通过导线与正导线的导体电连接。 封装安装部分，发光晶片和导线的透明外密封胶提供在LED灯串上。

公开(公告)号：US20080312173A1

公开(公告)日：2008-12-18

申请号：US12009825

申请日：2008-01-22

申请人： Yin Chen ,  Malcolm Skolnick

发明人： Yin Chen ,  Malcolm Skolnick

IPC分类号： A61K31/711 ,  A61P31/12

CPC分类号： C12N15/1133 ,  A61K2121/00 ,  C12N15/10 ,  C12N15/113 ,  C12N15/85 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2310/15 ,  C12N2310/16 ,  C12N2310/53 ,  C12N2840/20

摘要： A composition for treatment of HSV-related pathologies including an expression vector for altering expression of a target sequence in an HSV-infected cell by production of single-stranded cDNA (ssDNA) in the cell in vivo suspended for topical application to an affected site in a suitable delivery vehicle. The expression vector is comprised of a cassette comprising a sequence of interest, an inverted tandem repeat, and a primer binding site 3′ to the inverted tandem repeat, and a reverse transcriptase/RNAse H coding gene, and is transfected into the infected cells for inhibition of HSV replication. The resulting ssDNA binds to the target sequence to alter expression of the target sequence for such purposes as gene activation or inactivation using duplex or triplex binding of nucleic acids, site-directed mutagenesis, interruption of cellular function by binding to specific cellular proteins, or interfering with RNA splicing functions.

摘要翻译： 一种用于治疗HSV相关病状的组合物，其包括用于通过在体内悬浮用于局部施用于受影响部位的细胞中的单链cDNA（ssDNA）产生来改变HSV感染细胞中靶序列表达的表达载体 合适的运送工具。 表达载体由包含感兴趣序列，反向串联重复序列和反向串联重复序列的引物结合位点3'和逆转录酶/ RNA酶H编码基因的盒组成，并转染入感染的细胞中 抑制HSV复制。 所得的ssDNA结合靶序列以改变靶序列的表达，用于诸如基因激活或失活的目的，使用核酸的双链或三重结合，定点突变，通过结合特异性细胞蛋白中断细胞功能或干扰 具有RNA拼接功能。

公开(公告)号：US20080305142A1

公开(公告)日：2008-12-11

申请号：US11792800

申请日：2005-12-12

申请人： Yin Chen ,  Frederic Kendirgi ,  Malcolm Skolnick

发明人： Yin Chen ,  Frederic Kendirgi ,  Malcolm Skolnick

IPC分类号： A61F2/04 ,  C12P19/34 ,  A01N43/00 ,  A61K39/00 ,  A61P31/12 ,  A61P31/10 ,  A61K31/7052 ,  A61K9/12

CPC分类号： C12P19/34

摘要： The invention provides an improved cell free amplification method capable of producing large quantities of therapeutic-quality nucleic acids and methods of using the synthesized nucleic acid in research, therapeutic and other applications—The methods combine several different state-of-the-art procedures and coordinate their applications to affordably synthesize nucleic acids for therapeutic purposes. It combines in vitro rolling circle amplification, high fidelity polymerases, high affinity primers, and streamlined template specifically designed for particular applications. For expression purposes, the templates contain an expression cassette including a eukaryotic promoter, the coding sequence for the gene of interest, and a eukaryotic termination sequence. Following amplification, concatamers are subsequently processed according to their intended use and may include: restriction enzyme digestion for the production of short expression cassettes (SECs); ligation steps to circularize the SEC (CNAs); and/or supercoiling steps to produce sCNAs. The final product contains nearly non-detectable levels of bacterial endotoxin.

摘要翻译： 本发明提供了能够产生大量治疗质量核酸的改进的无细胞扩增方法和在研究，治疗和其它应用中使用合成核酸的方法 - 该方法结合了几种不同的最先进的方法和 协调其应用，以便为治疗目的负担得起的合成核酸。 它结合体外滚环扩增，高保真聚合酶，高亲和力引物和专为特定应用设计的流线型模板。 为了表达目的，模板含有包含真核启动子，目的基因的编码序列和真核终止序列的表达盒。 扩增后，随后根据其预期用途处理连接体，并且可以包括：用于产生短表达盒（SEC）的限制酶消化; 结转步骤，循环SEC（CNA）; 和/或超螺旋步骤以产生sCNA。 最终产品含有几乎不可检测的细菌内毒素水平。

公开(公告)号：US20080017816A1

公开(公告)日：2008-01-24

申请号：US11491836

申请日：2006-07-24

申请人： Robin Willats ,  Joseph Callahan ,  Terry Holley ,  Yin Chen

发明人： Robin Willats ,  Joseph Callahan ,  Terry Holley ,  Yin Chen

IPC分类号： F16K31/44

CPC分类号： F16K1/22 ,  F02D9/04 ,  F02D9/08 ,  F02D9/1065 ,  F02D9/1085 ,  F16K49/00

摘要： An exhaust valve assembly includes a valve body, a valve supported by a shaft within the valve body, and an actuator that drives the shaft to move the valve relative to the valve body. A thermal isolator is associated with the shaft to reduce heat transfer from the valve to the actuator.

摘要翻译： 排气门组件包括阀体，由阀体内的轴支撑的阀和驱动轴以相对于阀体移动阀的致动器。 热隔离器与轴相关联，以减少从阀到致动器的热传递。

公开(公告)号：US20070118693A1

公开(公告)日：2007-05-24

申请号：US11282201

申请日：2005-11-19

申请人： Karen Brannon ,  Yin Chen

发明人： Karen Brannon ,  Yin Chen

IPC分类号： G06F12/00 ,  G06F12/16

CPC分类号： G06F11/1666 ,  G06F11/1441 ,  G06F12/0866

摘要： A storage system has a cache and a main storage for longer term storage. The main storage has first files stored therein. First copies of a subset of the first files are cached responsive to user requests for ones of the first files. In a predetermined, set-aside portion of the main storage, substantially all the cached files are copied, so that the main storage includes the first files and second copies of substantially all of the subset of the first files. The second copies are in a more compact data structure in the set-aside portion than is the subset of the first files in a non-set-aside portion of the main storage. Also, ones of the second copies of the subset of the first files are loaded to the cache from the set-aside portion of the main storage in response to a loss of ones of the files in the cache.

摘要翻译： 存储系统具有缓存和用于长期存储的主存储。 主存储器中存储有第一个文件。 响应于对第一个文件的用户请求，缓存第一个文件的子集的第一个副本。 在主存储器的预定的预置部分中，基本上所有缓存的文件被复制，使得主存储器包括第一文件和第一文件的基本上所有子集的第二副本。 第二副本在设置旁边部分中比在主存储器的非预留部分中的第一个文件的子集更紧凑的数据结构。 此外，响应于高速缓存中的一个文件的丢失，第一文件的子集的第二副本中的一个被从主存储器的预留部分加载到高速缓存。

公开(公告)号：US20050125213A1

公开(公告)日：2005-06-09

申请号：US10729362

申请日：2003-12-04

申请人： Yin Chen ,  Rhonda Childress ,  Catherine Crawford ,  Noshir Wadia ,  Peng Ye

发明人： Yin Chen ,  Rhonda Childress ,  Catherine Crawford ,  Noshir Wadia ,  Peng Ye

IPC分类号： G06F9/45

CPC分类号： G06F11/3414 ,  G06F11/3447 ,  G06F11/3495

摘要： An apparatus, system, and method are provided for modeling and analyzing a plurality of computing workloads. The apparatus, system, and method include a data collection module for gathering performance data associated with operation of the computer system. A modeling module executes a plurality of models in parallel, in series, or according to a hierarchical relationship. A data analysis module presents analysis data compiled from the modeling module to a user, typically in the form of a graph. And finally, a framework manages the data collection module, the modeling module, and the data analysis module according to a predefined data and model flow.

摘要翻译： 提供了用于建模和分析多个计算工作负载的装置，系统和方法。 装置，系统和方法包括用于收集与计算机系统的操作相关的性能数据的数据收集模块。 建模模块并行，串联或根据分层关系执行多个模型。 数据分析模块将从建模模块编译的分析数据呈现给用户，通常以图形的形式。 最后，框架根据预定义的数据和模型流程管理数据收集模块，建模模块和数据分析模块。

公开(公告)号：US5854130A

公开(公告)日：1998-12-29

申请号：US864073

申请日：1997-05-28

申请人： Fu-Liang Yang ,  Yin Chen

发明人： Fu-Liang Yang ,  Yin Chen

IPC分类号： H01L21/768 ,  H01L23/522 ,  H01L21/4763

CPC分类号： H01L21/768 ,  H01L21/76801 ,  H01L21/76802 ,  H01L23/5226 ,  H01L2924/0002

摘要： A method for forming multilevel interconnects in a semiconductor IC device is provided. The method involves a simplified planarization process for planarization of inter-metal dielectrics that allows for easy and cost-effective fabrication of the device. By this method, an insulating layer is formed over a substrate, then a first conductive layer is formed over the insulating layer and which is selectively removed to form conductive interconnects. Subsequently, a dielectric layer is formed over the conductive interconnects. A photoresist layer is then formed and patterned over the dielectric layer by a spin-coating process. An etching process is then conducted on the photoresist layer and the dielectric layer with a 1:1 etching ratio until the photoresist layer is completely removed. At the same moment when the photoresist layer is completely removed, the via holes are formed. The following steps are the same for fabricating the next-level interconnects. In the foregoing method, the spin-coating process allows the photoresist layer to be formed with a flat top surface. In the etching process, the 1:1 etching ratio on the photoresist layer and the dielectric layer allows the underlying dielectric layer to have a flat top surface when the photoresist layer is completely removed. The planarization process is significantly simplified. This allows the manufacturing costs to be significantly reduced.

摘要翻译： 提供了一种在半导体IC器件中形成多层互连的方法。 该方法涉及用于平坦化金属间电介质的简化平面化处理，其允许容易且成本有效地制造器件。 通过这种方法，在衬底上形成绝缘层，然后在绝缘层上形成第一导电层，并且被选择性地去除以形成导电互连。 随后，在导电互连上形成介电层。 然后通过旋涂方法在介电层上形成并图案化光致抗蚀剂层。 然后在光致抗蚀剂层和电介质层上以1：1的蚀刻比进行蚀刻处理，直到光致抗蚀剂层被完全去除。 在光刻胶层完全除去的同时，形成通孔。 以下步骤与制造下一级互连相同。 在上述方法中，旋涂方法允许光致抗蚀剂层形成为平坦的顶表面。 在蚀刻过程中，当光致抗蚀剂层被完全去除时，光致抗蚀剂层和电介质层上的1：1蚀刻比允许下面的介电层具有平坦的顶表面。 平坦化过程显着简化。 这允许制造成本显着降低。