公开(公告)号：US20210041445A1

公开(公告)日：2021-02-11

申请号：US16979742

申请日：2019-03-13

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574

Abstract: Means for monitoring treatment response and disease progression in subjects are disclosed, where the predictions are based on the change of number and/or size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological ample, such as blood, from the subject.

公开(公告)号：US20200049713A1

公开(公告)日：2020-02-13

申请号：US16498581

申请日：2018-04-02

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  G01N33/50

Abstract: The characterization of nucleic acids obtained from cancer-associated cells circulating in the blood of a subject, and the use of such characterizations in cancer screening, diagnostics, treatment, and recurrence, are disclosed.

公开(公告)号：US20190262778A1

公开(公告)日：2019-08-29

申请号：US16265305

申请日：2019-02-01

Applicant: Creatv MicroTech, Inc.

Inventor： Cha-Mei Tang ,  Olga Makarova

IPC: B01D67/00 ,  A61M1/34 ,  B01D69/12 ,  C12M1/00 ,  B01D71/46 ,  B01D71/64

Abstract: Micro- and nanofilters with precision pore sizes and pore layout have applications in many fields including capturing circulating tumor cells and fetal cells in blood, water treatment, pathogen detection in water, etc. Methods to fabricate micro- and nanofilters not using track etching or reactive ion etching are provided, allowing easy fabrication of single layer or stack of films simultaneously, and/or stack of films on rolls. Microfilter can be made using one or more layers of material. Invention enables mass production of microfilters with lithographic quality at low cost. Isolation, enumeration and characterization of circulating tumor cells using microfilters provides (i) guides to cancer treatment selection and personalize dosage, (ii) low cost monitoring for treatment response, disease progression and recurrence, (iii) assessment of pharmacodynamic effects, (iv) information on mechanisms of resistance to therapy, and (v) cancer staging. Microfabrication methods are also applicable to fabrication of any free standing patterned polymeric films.

公开(公告)号：US20190242871A1

公开(公告)日：2019-08-08

申请号：US16369730

申请日：2019-03-29

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/49 ,  G01N33/574 ,  G01N33/487

CPC classification number: G01N33/491 ,  G01N33/48735 ,  G01N33/57415 ,  G01N33/57419 ,  G01N33/57423 ,  G01N33/57434

Abstract: A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of solid tumors, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

公开(公告)号：US20190128892A1

公开(公告)日：2019-05-02

申请号：US16092998

申请日：2017-04-14

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  C07K16/28 ,  A61K39/395 ,  A61K45/06

Abstract: Methods of using detection of PD-L1 expression by circulating cancer cells in the screening, monitoring, treatment and diagnosis of cancer in subjects are disclosed. The methods are based on assaying one or more of circulating tumor cells (CTCs), epithelial to mesenchymal transition CTCs (EMTCTCs), cancer associated macrophage-like cells (CAMLs), and cancer associated vascular endothelial cells (CAVEs) isolated from a subject having cancer for PD-L1 expression.

公开(公告)号：US20180156801A1

公开(公告)日：2018-06-07

申请号：US15576472

申请日：2016-05-26

Applicant: CREATV MICROTECH INC.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  G01N33/50

Abstract: Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.

公开(公告)号：US20160021872A1

公开(公告)日：2016-01-28

申请号：US14776194

申请日：2014-03-13

Applicant: CREATV MICROTECH, INC.

Inventor： Peixuan ZHU ,  Daniel ADAMS ,  Cha-Mei TANG

IPC: A01N1/02 ,  G01N33/493 ,  G01N33/574

CPC classification number: A01N1/021 ,  A01N1/0215 ,  G01N33/493 ,  G01N33/574

Abstract: A preservative reagent for urine is disclosed that increases the stability of cells, such as tumor cells, in urine for a period of several weeks. The preservative reagent comprises polyethylene glycol (PEG), ethanol, paraformaldehyde (PFA), and ethylenediaminetetraacetic acid (EDTA), and optionally pH stabilizing reagents.

Abstract translation: 公开了用于尿液的防腐剂，其在尿液中增加细胞（例如肿瘤细胞）的稳定性，持续数周。 防腐剂包括聚乙二醇（PEG），乙醇，多聚甲醛（PFA）和乙二胺四乙酸（EDTA），以及任选的pH稳定试剂。

公开(公告)号：US20130330721A1

公开(公告)日：2013-12-12

申请号：US13854003

申请日：2013-03-29

Applicant: Creatv Micro Tech, Inc.

Inventor： Cha-Mei Tang ,  Yunqi Zhang

IPC: G01N1/40 ,  B01D29/00

CPC classification number: G01N33/5005 ,  B01D29/00 ,  B01D39/16 ,  B01D39/1692 ,  B01L3/50 ,  B01L3/502 ,  B01L3/5635 ,  B01L9/00 ,  B01L2300/0681 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088 ,  Y10T29/49826 ,  Y10T29/49963

Abstract: A microfilter comprising a polymer layer formed from photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. Methods of forming apertures in one or more layers of photo-definable dry film are also disclosed. Filter holder designs and methods appropriate to hold microfilters to collect the rare cells and to perform of assays in the filter holder are provided. Microfiltration chip designs and methods appropriate to collect the rare cells and to perform assays in the microfluidic chips are provided. The invention also describes the use of the microfilter, filter holder and microfilter chips to collect rare cells from body fluids and perform assays, and these rare cells can be used for medical and biological research applications.

Abstract translation: 一种包含由光可定义干膜形成的聚合物层的微过滤器和各自延伸穿过聚合物层的多个孔。 包括由可光限定的干膜形成的两个或更多个聚合物层的微滤器以及各自延伸穿过聚合物层的多个孔或开口区域。 还公开了在一个或多个光可定义干膜层中形成孔的方法。 提供了适用于容纳微型过滤器以收集稀有细胞并在过滤器保持器中执行测定的过滤器支架设计和方法。 提供了适用于收集稀有细胞并在微流控芯片中进行测定的微滤芯片设计和方法。 本发明还描述了微型过滤器，过滤器保持器和微过滤器芯片用于从体液中收集稀有细胞并进行测定的用途，并且这些稀有细胞可用于医学和生物研究应用。

公开(公告)号：US08351741B2

公开(公告)日：2013-01-08

申请号：US12494152

申请日：2009-06-29

Applicant: Cha-Mei Tang ,  Platte T. Amstutz, III

Inventor： Cha-Mei Tang ,  Platte T. Amstutz, III

IPC: G02B6/32 ,  G02B6/42 ,  G02B6/00

CPC classification number: G01N21/0303 ,  G01N21/255 ,  G01N21/645 ,  G01N2021/6473

Abstract: A method of detecting fluorescence/absorbance/luminescence from 24-well, 48-well, 96-well, 384-well and 1536-well microplates and other sample containers. The sample is pumped into a waveguide. The waveguide efficiently gathers and guides the emission light to the end of the waveguide. The emission light exits the ends of the waveguide and is focused into a detector. To minimize background caused by the excitation light used for fluorescence, the excitation illuminates the waveguides at 90 degrees. To facilitate reuse, the waveguide assembly can be configured to be washed by an appropriate wash solution.

Abstract translation: 从24孔，48孔，96孔，384孔和1536孔微孔板和其他样品容器中检测荧光/吸光度/发光的方法。 将样品泵送到波导中。 波导有效地将发射光聚集并引导到波导的末端。 发射光离开波导的端部并聚焦到检测器中。 为了最小化用于荧光的激发光引起的背景，激发以90度照射波导。 为了便于重新使用，波导组件可以被配置为被适当的洗涤溶液洗涤。

公开(公告)号：US07565042B2

公开(公告)日：2009-07-21

申请号：US11705606

申请日：2007-02-13

Applicant: Cha-Mei Tang ,  Platte T Amstulz, III

Inventor： Cha-Mei Tang ,  Platte T Amstulz, III

IPC: G02B6/32 ,  G02B6/42 ,  G02B6/00

CPC classification number: G01N21/0303 ,  G01N21/255 ,  G01N21/645

Abstract: A luminometer is provided comprising a flow through waveguide and one or more detectors. The flow through waveguide has at least two openings and the sample is free to enter from one opening and exit from the other. The flow through waveguide can be made of material that guides emission light to a bottom end of the flow through waveguide. One or more detectors may be provided which detect the emission light coming out of the bottom of the flow through waveguide. A fluorometer/photometer is also provided that comprises a flow through waveguide, one or more excitation light sources, and one or more optical detectors. The flow through waveguide has a hollow region to hold the sample. The excitation light is introduced at an angle or perpendicular to one surface of the flow through waveguide. The flow through waveguide is made of material that can guide absorption and/or emission light to the bottom end of the flow through waveguide. There are one or more detectors that detect the emission light coming out of the bottom of the flow through waveguide.

Abstract translation: 提供了一种发光计，其包括流过波导管和一个或多个检测器。 通过波导的流动具有至少两个开口，并且样品自由地从一个开口进入并从另一个开口排出。 通过波导的流动可以由将发射光引导到通过波导的流动的底端的材料制成。 可以提供一个或多个检测器，其检测从通过波导的流的底部出射的发射光。 还提供了一种荧光计/光度计，其包括流过波导管，一个或多个激发光源和一个或多个光学检测器。 通过波导的流动具有保持样品的中空区域。 激发光以与通过波导的流动的一个表面成一角度或垂直的方式引入。 通过波导的流动由能够将吸收和/或发射光引导到通过波导的流动的底端的材料制成。 存在一个或多个检测器，其检测从通过波导的流动的底部出来的发射光。