公开(公告)号：US20090226969A1

公开(公告)日：2009-09-10

申请号：US12087289

申请日：2006-12-28

Applicant: Jan Johansson ,  Göran Hjälm ,  Margareta Stark ,  Anna Rising ,  Stefan Grip ,  Wilhelm Engström ,  My Hedhammar

Inventor： Jan Johansson ,  Göran Hjälm ,  Margareta Stark ,  Anna Rising ,  Stefan Grip ,  Wilhelm Engström ,  My Hedhammar

IPC: C12P21/00 ,  C07K14/435 ,  C07H21/00 ,  C07K1/107

CPC classification number: C07K14/43518 ,  A61K35/12 ,  A61K47/42 ,  D01F4/00

Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula KEP-CT. KEP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

Abstract translation: 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式KEP-CT定义。 KEP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。

公开(公告)号：US20230070786A1

公开(公告)日：2023-03-09

申请号：US17895320

申请日：2022-08-25

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Anna RISING ,  Jan JOHANSSON ,  Marlene ANDERSSON

IPC: C07K14/435 ,  C12N5/00

Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D.

公开(公告)号：US20210069939A1

公开(公告)日：2021-03-11

申请号：US16644772

申请日：2018-09-26

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Linnea Gustafsson ,  Ronnie Jansson ,  Wouter van der Wijngaart

IPC: B29C39/02 ,  C07K14/435 ,  B29C33/42 ,  B29C39/10 ,  B29C41/12 ,  C09D127/12

Abstract: A method for manufacturing shaped polymers of surface-active macromolecules, in particular silk, is provided. The method is comprising the steps of: • a) depositing an aqueous solution of the surface-active macromolecules on a surface, wherein the aqueous solution of the surface-active macromolecules is deposited in the form of a droplet, and wherein the surface is a hydrophobic micropatterned surface adapted to prevent the aqueous solution from penetrating into the pattern and to receive the droplet of the aqueous solution of the surface-active macromolecules and retain its droplet state; and • b) forming shaped polymers of the surface-active macromolecules on the surface.

公开(公告)号：US20190322710A1

公开(公告)日：2019-10-24

申请号：US16403780

申请日：2019-05-06

Applicant: Spiber Technologies AB

Inventor： My Hedhammar

IPC: C07K14/435 ,  C12N5/00 ,  C08H1/00 ,  C12N15/62

Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for α5β1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.

公开(公告)号：US20190248847A1

公开(公告)日：2019-08-15

申请号：US16314501

申请日：2017-06-29

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Anna Rising ,  Jan Johansson ,  Marlene Andersson

IPC: C07K14/435 ,  C12N5/00 ,  B33Y70/00

CPC classification number: C07K14/43518 ,  B33Y70/00 ,  C12N5/0018 ,  C12N5/0062 ,  C12N2513/00 ,  C12N2533/50

Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NO:s 62-65 or 67-73; and a sequence having at least 70% identity to SEQ. ID NO: 64 or any one of SEQ ID NO:s 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D. Solutions, polymers, uses and methods of manufacture thereof.

公开(公告)号：US09309299B2

公开(公告)日：2016-04-12

申请号：US14753101

申请日：2015-06-29

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC: C07K14/435 ,  A61K47/48 ,  B01D15/38 ,  B01J20/281 ,  C07K16/06 ,  B01D15/20 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C12N5/00

CPC classification number: C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

Abstract: A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

公开(公告)号：US20150291674A1

公开(公告)日：2015-10-15

申请号：US14753101

申请日：2015-06-29

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Jan JOHANSSON ,  Anna RISING ,  Per Åke NYGREN

IPC: C07K14/435 ,  C07K14/76 ,  B01D15/20 ,  C07K14/31 ,  C12N5/00 ,  C07K16/06 ,  C07D495/04

CPC classification number: C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

Abstract: A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

Abstract translation: 提供能够与有机靶相互选择性的蛋白质结构。 蛋白质结构是包含能够与有机靶选择性相互作用的重组融合蛋白作为重复结构单元的聚合物。 融合蛋白包含部分B，REP和CT，以及任选的NT。 B是超过30个氨基酸残基的非宿主部分，其提供与有机靶的选择性相互作用的能力。 REP是70至300个氨基酸残基的部分，并衍生自蜘蛛丝蛋白的重复片段。 CT是70至120个氨基酸残基的部分，衍生自蜘蛛丝蛋白的C末端片段。 NT是100至160个氨基酸残基的任选部分，并衍生自蜘蛛丝蛋白的N-末端片段。 其融合蛋白质和蛋白质结构可用作亲和介质和细胞支架材料。

公开(公告)号：US09115204B2

公开(公告)日：2015-08-25

申请号：US13880628

申请日：2011-10-25

Applicant: My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

Inventor： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC: C07K14/435 ,  A61K47/48 ,  B01D15/38 ,  B01J20/281 ,  C07K16/06

CPC classification number: C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

Abstract: A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

Abstract translation: 提供能够与有机靶相互选择性的蛋白质结构。 蛋白质结构是包含能够与有机靶选择性相互作用的重组融合蛋白作为重复结构单元的聚合物。 融合蛋白包含部分B，REP和CT，以及任选的NT。 B是超过30个氨基酸残基的非宿主部分，其提供与有机靶的选择性相互作用的能力。 REP是70至300个氨基酸残基的部分，并衍生自蜘蛛丝蛋白的重复片段。 CT是70至120个氨基酸残基的部分，衍生自蜘蛛丝蛋白的C末端片段。 NT是100至160个氨基酸残基的任选部分，并衍生自蜘蛛丝蛋白的N-末端片段。 其融合蛋白质和蛋白质结构可用作亲和介质和细胞支架材料。

公开(公告)号：US20150119554A1

公开(公告)日：2015-04-30

申请号：US14398361

申请日：2013-05-02

Applicant: SPIBER TECHNOLOGIES AB ,  IMMUNOVIA AB

Inventor： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC: C07K14/78 ,  C07K16/44

CPC classification number: C07K14/78 ,  C07K14/43518 ,  C07K16/00 ,  C07K16/44 ,  C07K2317/35 ,  C07K2317/56 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/30 ,  C07K2319/70

Abstract: A recombinant fusion protein comprising the moieties Band CT, and optionally REP, wherein B is comprising at least one immunoglobulin fragment, which provides the capacity of selective interaction with an organic target; CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein; and REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein.

Abstract translation: 包含部分带CT和任选REP的重组融合蛋白，其中B包含至少一个免疫球蛋白片段，其提供与有机靶的选择性相互作用的能力; CT是70至120个氨基酸残基的部分，衍生自蜘蛛丝蛋白的C末端片段; 并且REP是70至300个氨基酸残基的部分，并衍生自蜘蛛丝蛋白的重复片段。

公开(公告)号：US08618255B2

公开(公告)日：2013-12-31

申请号：US13598119

申请日：2012-08-29

Applicant: Jan Johansson ,  Göran Hjäm ,  Margareta Stark ,  Anna Rising ,  Stefan Grip ,  Wilhelm Engström ,  My Hedhammar

Inventor： Jan Johansson ,  Göran Hjäm ,  Margareta Stark ,  Anna Rising ,  Stefan Grip ,  Wilhelm Engström ,  My Hedhammar

IPC: C07K1/00 ,  C07K14/00

CPC classification number: C07K14/43518 ,  A61K35/12 ,  A61K47/42 ,  D01F4/00

Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

Abstract translation: 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式REP-CT定义。 REP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。