Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation

    公开(公告)号:US11597740B2

    公开(公告)日:2023-03-07

    申请号:US17097488

    申请日:2020-11-13

    Inventor: Stefan Jennewein

    Abstract: The present application discloses a simple process for the purification of neutral human milk oligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of cationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and/or electrodialysis step, which allows efficient purification of large quantities of neutral HMOs at high purity. Contrary to the purification currently used in fermentative production of neutral HMOs, the presented process allows the provision of HMOs without the need of a chromatographic separation. The so purified HMOs may be obtained in solid form by spray drying, as crystalline material or as sterile filtered concentrate. The provided HMOs are free of proteins and recombinant material originating from the used recombinant microbial strains and thus very well-suited for use in food, medical food and feed (e.g. pet food) applications.

    SWEETENER COMPOSITION
    37.
    发明申请

    公开(公告)号:US20230061329A1

    公开(公告)日:2023-03-02

    申请号:US17905958

    申请日:2021-03-09

    Abstract: The present invention relates to a sweetener composition and a process for preparing a sweetener composition. In particular, the present invention relates to a sweetener composition comprising a crystalline carbohydrate and a bulking agent for replacing all or part of the sugar in a food product. The bulking agent can be selected from soluble and insoluble fibres.

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