XYLANASE MUTANT AND ITS PREPARATION METHOD AND APPLICATION

    公开(公告)号:US20220251527A1

    公开(公告)日:2022-08-11

    申请号:US17219550

    申请日:2021-03-31

    Abstract: The present invention discloses a kind of xylanase mutant and its preparation method and application, which relates to the technical field of genomic engineering and genetic engineering. Such mutant includes one or more mutants obtained by taking xylanase HwXyl10A as female parent to conduct saturation mutagenesis to the site of Gly363. Specifically, relates to obtaining 19 mutants through site-directed mutagenesis, and then conducting yeast expression to them, after that, obtaining two mutants with significantly improved specific activity and thermal stability through screening of thermal stability and catalytic activity; the present invention can significantly improve the thermal stability and catalytic efficiency of xylanase through modifying the site of Gly363, and is of important guiding significance for improving the thermal stability and the catalytic efficiency of the 10th family of xylanases and other glycoside hydrolases as well as lays the foundation for its application in industrial production.

    Complex pressure environment test device

    公开(公告)号:US11105721B1

    公开(公告)日:2021-08-31

    申请号:US17273598

    申请日:2019-08-26

    Abstract: A complex pressure environment test device, comprising a test table and a hydraulic control system. The test table comprises a base for fastening a closure head under test, and an outer cover; the base has water inlets and outlets. The hydraulic control system comprises a watering hydraulic pump, a pressurizing hydraulic pump, and a pilot-operated overflow valve; the watering hydraulic pump is separately connected to a first two-position two-way valve and a second two-position two-way valve; the second two-position two-way valve is connected to a second one-way valve; the second one-way valve is separately connected to a water inlet and a first pilot-operated pressure regulating valve; the first pilot-operated pressure regulating valve is separately connected to a first remote pressure regulating valve and a first one-way valve. There is also a hydraulic circuit is present and used for testing.

    COMPLEX PRESSURE ENVIRONMENT TEST DEVICE

    公开(公告)号:US20210247282A1

    公开(公告)日:2021-08-12

    申请号:US17273598

    申请日:2019-08-26

    Abstract: A complex pressure environment test device, comprising a test table and a hydraulic control system. The test table comprises a base for fastening a closure head under test, and an outer cover; the base has water inlets and outlets. The hydraulic control system comprises a watering hydraulic pump, a pressurizing hydraulic pump, and a pilot-operated overflow valve; the watering hydraulic pump is separately connected to a first two-position two-way valve and a second two-position two-way valve; the second two-position two-way valve is connected to a second one-way valve; the second one-way valve is separately connected to a water inlet and a first pilot-operated pressure regulating valve; the first pilot-operated pressure regulating valve is separately connected to a first remote pressure regulating valve and a first one-way valve. There is also a hydraulic circuit is present and used for testing.

    INVERTED NON-STOP APPARATUS OF MULTI-CYLINDER DIESEL ENGINE FOR FREE-FALL LIFEBOAT

    公开(公告)号:US20200291832A1

    公开(公告)日:2020-09-17

    申请号:US16500093

    申请日:2017-12-01

    Abstract: An inverted non-stop apparatus of a multi-cylinder diesel engine for a free-fall lifeboat is provided. The apparatus includes a lubricating system and a breathing system. The lubricating system includes a machine body (101), an engine oil pump, and an oil pan (118). The breathing system is a diesel engine breather. A partition board (1181) is transversely provided in the oil pan (118) to divide a cavity of the oil pan (118) into a left cavity and a right cavity. The left cavity is an oil collection groove (1182), and the right cavity is an oil tank body (127A). A flange is provided at an opening of the oil tank body (127A) for fixing an oil tank cover (125) to form an enclosed lubricating oil tank (127). The engine oil pump is a double pump having oil inlets and outlets independent separately, which is formed by superposing upper and lower layers, wherein the upper layer is a lubricating pump (111) and the lower layer is an oil return pump (112). An oil suction port (114) of the oil return pump (112) is connected to the bottom of the oil collection groove (1182), and an oil outlet is connected to the lubricating oil tank (127). An oil inlet of the lubricating pump is connected to the lubricating oil tank (127), and an oil outlet is communicated with a main oil path (102) of the machine body. A breathing one-way valve (132) and an air pressure relief valve (131) are provided on the oil tank cover (125). The inverted non-stop apparatus of a multi-cylinder diesel engine for a free-fall lifeboat separate an engine oil collection function and an engine oil storage function borne by an oil pan of a diesel engine, so that no matter the diesel engine is longitudinally and transversely turned over and inverted, the diesel engine can operate normally.

    Automobile brake assembly fatigue test stand

    公开(公告)号:US10156497B2

    公开(公告)日:2018-12-18

    申请号:US15523747

    申请日:2016-03-25

    Abstract: The invention discloses an automobile brake assembly fatigue test stand, which includes a base and a thermotank, wherein a mandrel is mounted inside the thermotank, two ends of the mandrel are respectively inserted into a first taper sleeve and a second taper sleeve, the first taper sleeve is mounted inside a hollow brake tooling, one end of the brake tooling is connected with a first bearing pedestal casing, the other end of the brake tooling is connected with a brake, the first bearing pedestal casing is sequentially connected with a brake shaft, a static torque sensor and a support; the second taper sleeve is mounted inside a brake drum tooling through a bearing, one end of the brake drum tooling is connected with a brake drum sleeved on the mandrel while the other end is connected with a brake drum shaft, and the brake drum shaft is sequentially connected with a second bearing pedestal casing, a coupling, a reducer and a first servo motor. The mandrel is additionally mounted between two tooling fixtures, the tooling fixtures can freely rotate around the mandrel and support the two tooling fixtures, so that the rigidity of the tooling fixtures is enhanced, and the stability during testing is guaranteed.

    SELF-BALANCED PRESSURE HULL DEVICE
    48.
    发明申请

    公开(公告)号:US20180237108A1

    公开(公告)日:2018-08-23

    申请号:US15752586

    申请日:2016-08-09

    CPC classification number: B63B3/13 B63G8/001 B63G8/24 B63G8/26

    Abstract: A self-balanced pressure hull device, belonging to the field of pressure structure technology of deep-sea submersibles, being assembled by nesting, from inside to outside, a spherical inner housing, a spherical intermediate housing and a spherical outer housing around the sphere centre, pairs of symmetric coaxial connecting shaft components being connected between the spherical inner housing and the spherical intermediate housing and between the spherical intermediate housing and the spherical outer housing, respectively; axes of the two pairs of connecting shaft components are perpendicular to each other so as to enable the spherical inner housing and the spherical intermediate housing to rotate relative to each other, and the spherical intermediate housing and the spherical outer housing to rotate relative to each other; and each of the connecting shaft components in the two pairs being provided with a spring damper for resisting the axial impact between each two adjacent housings.

    Bionic high holding power anchor
    49.
    发明授权

    公开(公告)号:US09937980B2

    公开(公告)日:2018-04-10

    申请号:US15523965

    申请日:2016-03-29

    CPC classification number: B63B21/34 B63B21/14 B63B2021/262 B63B2021/265

    Abstract: The present invention discloses a bionic high holding power anchor, which includes an anchor rod, a suspension clasp coupled with one end of the anchor rod and an anchor fluke hinged with the other end of the anchor rod, wherein a plurality of fluke heads arranged at equal intervals are extended from a front end of the anchor fluke, fin-like streamlined balance wings are arranged at two sides of the anchor fluke; the fluke head is in a shape of triangle-like taper containing a plane side wall and two curved side walls, the plane side wall is an isosceles triangle, the two curved side walls are smooth curved surfaces, and the two smooth curved surfaces are symmetrical around a normal plane of the plane side wall. According to the bionic high holding power anchor of the present invention, the balance wings are arranged at the two sides of the anchor fluke, a body is avoided from being turned completely and the anchor reaches to a soil layer at a correct working angle during a process that the anchor is thrown into water; and a longitudinal dimension of the anchor is increased, so that an anti-turning property of the anchor in the soil layer is improved significantly, and a risk of anchor dragging is reduced.

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