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    ANIMAL FEED ENZYME EXTRACTION
    41.
    发明申请
    ANIMAL FEED ENZYME EXTRACTION 审中-公开
    动物饲料酶提取

    公开(公告)号：US20150361478A1

    公开(公告)日：2015-12-17

    申请号：US14764142

    申请日：2014-01-28

    Applicant: BASF ENZYMES LLC

    Inventor： Cristina POP ,  Arne I. SOLBAK, JR. ,  Adrienne HUSTON DAVENPORT

    IPC: C12Q1/42

    CPC classification number: C12Q1/42 ,  C07K1/145 ,  C12Q1/25 ,  G01N33/573 ,  G01N2333/916

    Abstract: An in-feed assay and buffers for extracting an enzyme from a feed pellet treated at high temperature is provided. Another embodiment of the invention is measuring the enzyme activity of the enzyme additive extracted from the animal feed, measuring the quantity of enzyme extracted from the animal feed, or measuring both. In one embodiment the enzyme is an animal feed additive, such as a phytase.

    Abstract translation: 提供了进料测定法和用于从高温处理的进料颗粒中提取酶的缓冲液。 本发明的另一个实施方案是测量从动物饲料中提取的酶添加剂的酶活性，测量从动物饲料中提取的酶的量，或测量两者。 在一个实施方案中，酶是动物饲料添加剂，例如植酸酶。

    PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    42.
    发明申请
    PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
    PHYTASES，NUCLEIC ACID ENCODING THEM AND METHODS FOR MAKING AND USING THEM

    公开(公告)号：US20150132383A1

    公开(公告)日：2015-05-14

    申请号：US14504849

    申请日：2014-10-02

    Applicant: BASF ENZYMES, LLC

    Inventor： Arne SOLBAK ,  Brian STEER ,  Mark DYCAICO ,  Katie A. KLINE ,  Axel TREFZER ,  Thomas TODARO ,  Fatima EL-FARRAH ,  Alberto ALVARADO ,  Gerhard FREY

    IPC: C12N9/16 ,  A23K1/165 ,  A23L1/305

    CPC classification number: C12N9/16 ,  A23K20/189 ,  A23L33/18 ,  A23V2002/00 ,  C12P7/10 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072 ,  Y02E50/16 ,  Y02E50/17

    Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

    Abstract translation: 本发明涉及植酸酶，编码它们的多核苷酸，本发明的多核苷酸和多肽的用途，以及这些多核苷酸和多肽的产生和分离。 特别地，本发明提供了在高温条件下具有植酸酶活性的多肽，以及暴露于高温后保留活性的植酸酶。 除了较高的温度之外，本发明的植酸酶还可以在低温下耐热和/或耐热。 本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以配制成食物或饲料或补充剂，用于例如帮助消化植酸盐。 本发明的食品或饲料可以是丸粒，液体，粉末等的形式。 在一个方面，本发明的植酸酶在造粒期间对于热变性是稳定的; 并且这降低了植酸酶产品的成本，同时保持了体内功效并检测了饲料中的活性。

    PHYTASE
    49.
    发明申请
    PHYTASE 审中-公开

    公开(公告)号：US20200248154A1

    公开(公告)日：2020-08-06

    申请号：US16714393

    申请日：2019-12-13

    Applicant: BASF Enzymes LLC

    Inventor： Xuqiu TAN ,  Arne I. SOLBAK, Jr.

    IPC: C12N9/16

    Abstract: Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning.

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