公开(公告)号：US11460468B2

公开(公告)日：2022-10-04

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B25/10 ,  G16H20/13 ,  G16H10/20

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US11220685B2

公开(公告)日：2022-01-11

申请号：US16836750

申请日：2020-03-31

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Eleen Shum

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

Abstract: The present disclosure relates to compositions, methods and kits for labeling an internal sequence of a target nucleic acid molecule with molecular barcodes. In some embodiments, the methods comprise intramolecular circulation of a labeled target nucleic acid molecule. Further provided methods for generating sequencing libraries comprising overlapping fragments covering the full length of a target nucleic acid molecule, sequencing the libraries using the methods disclosed herein, and methods of analyzing sequencing results therefrom.

公开(公告)号：US10941396B2

公开(公告)日：2021-03-09

申请号：US14381488

申请日：2013-02-27

Applicant: Cellular Research, Inc.

Inventor： Glenn K. Fu ,  Stephen P. A. Fodor ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: Methods, kits and systems are disclosed for analyzing one or more molecules in a sample. Analyzing the one or more molecules may comprise quantitation of the one or more molecules. Individual molecules may quantitated by PCR, arrays, beads, emulsions, droplets, or sequencing. Quantitation of individual molecules may further comprise stochastic labeling of the one or more molecules with a plurality of oligonucleotide tags to produce one or more stochastically labeled molecules. The methods may further comprise amplifying, sequencing, detecting, and/or quantifying the stochastically labeled molecules. The molecules may be DNA, RNA and/or proteins.

公开(公告)号：US20200299672A1

公开(公告)日：2020-09-24

申请号：US16799464

申请日：2020-02-24

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li ,  Dennis Prosen

IPC: C12N15/10

Abstract: Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.

公开(公告)号：US20200173992A1

公开(公告)日：2020-06-04

申请号：US16789358

申请日：2020-02-12

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Olaf Zoellner

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US10669570B2

公开(公告)日：2020-06-02

申请号：US16012635

申请日：2018-06-19

Applicant: CELLULAR RESEARCH, INC.

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum

IPC: C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6837 ,  C12Q1/6834

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US20200149037A1

公开(公告)日：2020-05-14

申请号：US16677012

申请日：2019-11-07

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12N15/10 ,  C40B50/04 ,  C40B50/06 ,  C40B40/06

Abstract: Disclosed herein include systems, methods, compositions, and kits for whole transcriptome analysis (WTA) with random priming and extension (RPE). The RPE-based WTA method can comprise hybridizing random primers with a plurality of first strand barcoded polynucleotides associated with a solid support and extending the random primers to generate a plurality of extension products. The method can comprise amplifying the plurality of extension products to generate a sequencing library.

公开(公告)号：US20200058371A1

公开(公告)日：2020-02-20

申请号：US16540971

申请日：2019-08-14

Applicant: CELLULAR RESEARCH, INC.

Inventor： Christina Chang ,  Eleen Shum ,  Sixing Li ,  Margaret Nakamoto

IPC: G16B30/00 ,  G16B25/10 ,  C12Q1/6888

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification and protein expression profiling. A sample indexing composition, or a composition for protein expression profiling, can comprise, for example, a protein binding aptamer associated with an oligonucleotide, such as a sample indexing oligonucleotide. Different oligonucleotides can have different sequences. Sample origin of cells, or protein expression profiles of cells, can be determined based on the sequences of the oligonucleotides by, for example, barcoding the oligonucleotides.

公开(公告)号：US10338066B2

公开(公告)日：2019-07-02

申请号：US15993468

申请日：2018-05-30

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Olaf Zoellner

IPC: G01N33/53 ,  G01N33/566 ,  C12Q1/68 ,  G16B99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/543 ,  G01N33/50 ,  G01N33/68 ,  G06F19/00 ,  C12Q1/6804

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20190095578A1

公开(公告)日：2019-03-28

申请号：US16139699

申请日：2018-09-24

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Jue Fan ,  Jennifer Tsai

IPC: G06F19/24 ,  G06F19/22 ,  G06F19/28 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F17/18

Abstract: Disclosed herein are methods and systems for determining occurrences of targets. In some embodiments, the method comprises: collapsing putative sequences of the target; collapsing molecular label sequences associated with the putative sequences of the target; and estimating the occurrence of the target, wherein the occurrence of the target estimated correlates with the occurrence of molecular label sequences associated with the putative sequences of the target in the sequencing data after collapsing the occurrence of the putative sequences of the target and the occurrence of noise molecular label sequences.