公开(公告)号：US20200299672A1

公开(公告)日：2020-09-24

申请号：US16799464

申请日：2020-02-24

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li ,  Dennis Prosen

IPC: C12N15/10

Abstract: Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.

公开(公告)号：US20200058371A1

公开(公告)日：2020-02-20

申请号：US16540971

申请日：2019-08-14

Applicant: CELLULAR RESEARCH, INC.

Inventor： Christina Chang ,  Eleen Shum ,  Sixing Li ,  Margaret Nakamoto

IPC: G16B30/00 ,  G16B25/10 ,  C12Q1/6888

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification and protein expression profiling. A sample indexing composition, or a composition for protein expression profiling, can comprise, for example, a protein binding aptamer associated with an oligonucleotide, such as a sample indexing oligonucleotide. Different oligonucleotides can have different sequences. Sample origin of cells, or protein expression profiles of cells, can be determined based on the sequences of the oligonucleotides by, for example, barcoding the oligonucleotides.

公开(公告)号：US10722880B2

公开(公告)日：2020-07-28

申请号：US15868811

申请日：2018-01-11

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li

IPC: B01L3/00 ,  C12N15/10 ,  G01N15/10 ,  C12Q1/6874 ,  G01N15/14 ,  C12Q1/6869 ,  C12N15/09 ,  C12Q1/68

Abstract: Disclosed herein are methods, devices, and systems for efficient loading and retrieval of particles. In some embodiments, a fluidic channel of a flowcell comprises a ceiling, a first sidewall, and a bottom, wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall, and wherein the bottom of the fluidic channel comprises a substrate that comprises a plurality of microwells.

公开(公告)号：US20200217861A1

公开(公告)日：2020-07-09

申请号：US16824318

申请日：2020-03-19

Applicant: Cellular Research, Inc.

Inventor： Janice H. Lai ,  Philipp S. Spuhler ,  Geoffrey R. Facer ,  Sixing Li ,  Christopher G. Cesar

IPC: G01N35/00 ,  B01L3/00 ,  B01L9/00 ,  C12N15/10 ,  G01N35/10

Abstract: Disclosed herein are methods, devices, and systems for loading and retrieval of particles. In some embodiments, a loading station comprise a tray configured to receive a microwell array, a first magnet, a second magnet, and an actuation mechanism configured to cause movement of at least one of the first magnet and the second magnet.

公开(公告)号：US20190056415A1

公开(公告)日：2019-02-21

申请号：US15677827

申请日：2017-08-15

Applicant: Cellular Research, Inc.

Inventor： Janice H. Lai ,  Philipp S. Spuhler ,  Geoffrey R. Facer ,  Sixing Li ,  Christopher G. Cesar

IPC: G01N35/00 ,  C12N15/10 ,  G01N35/10

Abstract: Disclosed herein are methods, devices, and systems for loading and retrieval of particles. In some embodiments, a loading station comprise a tray configured to receive a microwell array, a first magnet, a second magnet, and an actuation mechanism configured to cause movement of at least one of the first magnet and the second magnet.

公开(公告)号：US20190056028A1

公开(公告)日：2019-02-21

申请号：US15677849

申请日：2017-08-15

Applicant: Cellular Research, Inc.

Inventor： Philipp S. Spuhler ,  Geoffrey R. Facer ,  Sixing Li ,  Christopher G. Cesar

IPC: F16J15/02 ,  F16J15/14

CPC classification number: F16J15/022 ,  B01L3/563 ,  B01L3/565 ,  B01L2200/023 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2300/0803 ,  B01L2300/0858 ,  B01L2300/123 ,  F16J15/14

Abstract: Disclosed herein are methods, devices, and systems for fluidic handling. In some embodiments, a gasket for providing a fluidic interface with a flowcell includes an inner cavity extending distally from a proximal end of the gasket, the inner cavity being defined by a plurality of inner surfaces sections, an inlet port positioned at a distal end of the inner cavity, an outlet port positioned at a distal end of the gasket, and a cannula extending between the inlet port and the outlet port; wherein at least some of the plurality of inner surface sections are tapered towards the distal end of the gasket to direct a pipette tip received within the gasket towards the inlet port of the gasket.

公开(公告)号：US20240263165A1

公开(公告)日：2024-08-08

申请号：US18627190

申请日：2024-04-04

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li ,  Dennis E. Prosen

IPC: C12N15/10 ,  B01L3/00

CPC classification number: C12N15/1003 ,  B01L3/5027

Abstract: Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.

公开(公告)号：US11976269B2

公开(公告)日：2024-05-07

申请号：US16799464

申请日：2020-02-24

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li ,  Dennis Prosen

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00

CPC classification number: C12N15/1003 ,  B01L3/5027

Abstract: Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.

公开(公告)号：US11061043B2

公开(公告)日：2021-07-13

申请号：US16824318

申请日：2020-03-19

Applicant: Cellular Research, Inc.

Inventor： Janice H. Lai ,  Philipp S. Spuhler ,  Geoffrey R. Facer ,  Sixing Li ,  Christopher G. Cesar

IPC: G01N1/00 ,  G01N35/00 ,  C12N15/10 ,  G01N35/10 ,  B01L9/00 ,  B01L3/00

Abstract: Disclosed herein are methods, devices, and systems for loading and retrieval of particles. In some embodiments, a loading station comprise a tray configured to receive a microwell array, a first magnet, a second magnet, and an actuation mechanism configured to cause movement of at least one of the first magnet and the second magnet.

公开(公告)号：US10527171B2

公开(公告)日：2020-01-07

申请号：US15677849

申请日：2017-08-15

Applicant: Cellular Research, Inc.

Inventor： Philipp S. Spuhler ,  Geoffrey R. Facer ,  Sixing Li ,  Christopher G. Cesar

IPC: F16J15/02 ,  B01L3/00 ,  F16J15/14 ,  B01L1/00

Abstract: Disclosed herein are methods, devices, and systems for fluidic handling. In some embodiments, a gasket for providing a fluidic interface with a flowcell includes an inner cavity extending distally from a proximal end of the gasket, the inner cavity being defined by a plurality of inner surfaces sections, an inlet port positioned at a distal end of the inner cavity, an outlet port positioned at a distal end of the gasket, and a cannula extending between the inlet port and the outlet port; wherein at least some of the plurality of inner surface sections are tapered towards the distal end of the gasket to direct a pipette tip received within the gasket towards the inlet port of the gasket.