公开(公告)号：US11061005B2

公开(公告)日：2021-07-13

申请号：US16604357

申请日：2018-04-16

申请人： Metabolon, Inc.

发明人： Klaus Peter Adam ,  Andrew James Thompson

IPC分类号： G01N27/622 ,  H01J49/16 ,  G01N30/72 ,  G01N33/493 ,  G01N33/68 ,  G01N30/02

摘要： A method for determining in a sample, by mass spectrometry, the presence, absence, or amount of one or more analytes is described herein. The run time is less than six minutes. The method includes subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more analytes, wherein the one or more analytes are derivatized prior to ionization; measuring, in a single injection, by mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and using the measured amount of the one or more ions to determine the amount of each of the one or more analytes in the sample.

公开(公告)号：US20210148924A1

公开(公告)日：2021-05-20

申请号：US17043165

申请日：2019-04-01

申请人： METABOLON, INC.

发明人： Lisa FORD ,  Kirk L. PAPPAN ,  Tiffany A. FREED ,  Kelsey M. EASON

IPC分类号： G01N33/68 ,  H01J49/00 ,  G01N33/49 ,  H01J49/04 ,  G01N30/72

摘要： A method for determining in a sample, by mass spectrometry, the amount of one or more analytes selected from the group consisting of 12,13-DiHOME, 3-hydroxybutyrate (BHBA), 3-hydroxyoctanoate, 3-methylglutarylcarnitine, 3-ureidopropionate, 7-alpha-hydroxy-4-cholesten-3-one (7-Hoca), citrate, fucose, fumarate, gamma-tocopherol, glutamate, glutarate, glycerol, glycochenodeoxycholate, glycocholate, hypoxanthine, maleate, malonate, mannose, orotate, 2,3-pyrdinedicarboxylate, ribose, serine, taurine, taurochenodeoxycholate, taurocholate, palmitoleate, linolenate, xanthine, xylitol, and combinations thereof is described. The method comprises subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more analytes; measuring, by mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and using the measured amount to determine the amount of each of the one or more analytes in the sample.

公开(公告)号：US20200231541A1

公开(公告)日：2020-07-23

申请号：US16650429

申请日：2018-09-27

申请人： Metabolon, Inc.

发明人： Qibo Zhang

IPC分类号： C07C323/54 ,  C07K16/44 ,  C07C319/18 ,  G01N30/72

摘要： The present invention provides a compound of formula (I), formula (II), formula (III), (IV) or a salt thereof, compositions and methods of making the compound, methods and reagents for measuring the compound, and kits using the same. The use of a compound of formula (I), formula (II), formula (III), or formula (IV) for assessing or monitoring kidney function in a subject, determining predisposition to developing reduced kidney function, classifying a subject according to level of kidney function, and diagnosing or monitoring chronic kidney disease is also described.

公开(公告)号：US10539575B2

公开(公告)日：2020-01-21

申请号：US14889930

申请日：2014-05-13

申请人： METABOLON, INC.

发明人： Regis Perichon ,  Jeffery Edmond Cobb ,  Meredith V. Brown ,  Adam Kennedy

IPC分类号： G01N33/68 ,  G01N33/70 ,  G01N33/64 ,  G01N33/53 ,  G01N30/02

摘要： Biomarkers of kidney function and methods for using said biomarkers for assessing kidney function, monitoring kidney function, diagnosing acute kidney injury, and diagnosing chronic kidney disease are provided. Also provided are suites of small molecule entities as biomarkers for chronic kidney disease.

公开(公告)号：US20170117122A1

公开(公告)日：2017-04-27

申请号：US15315147

申请日：2015-05-28

申请人： METABOLON, INC.

发明人： Hongping Dai ,  Corey Donald DeHaven

IPC分类号： H01J49/00 ,  G01N30/86 ,  H01J49/04

CPC分类号： H01J49/0036 ,  G01N30/8634 ,  G01N30/8644 ,  G01N33/6848 ,  H01J49/04

摘要： A method, apparatus, and computer-readable storage medium for analyzing sample data from a component separation/mass spectrometer system. A profile plot is formed for each sample, each having retention time and intensity axes, the intensity being represented as a function of retention time for a selected sample ion mass. An intensity peak arrangement, including at least one identifying peak, each having a peak range and characteristic intensity, is identified for a selected ion in the profile plot for each sample. An orthogonal plot, corresponding to the profile plot, for each sample is formed, extending along the retention time axis perpendicularly to the intensity axis. The characteristic intensity of each of the at least one identifying peak is represented on the retention time axis of the orthogonal plot with gradated indicia.

公开(公告)号：US20170089872A1

公开(公告)日：2017-03-30

申请号：US15288917

申请日：2016-10-07

申请人： Metabolon, Inc.

发明人： Michael V. Milburn ,  John A. Ryals ,  Lining Guo ,  Andrea Eckhart ,  Jacob Wulff ,  Adam D. Kennedy ,  Thomas J. Jönsson ,  Ryan Douglas Michalek ,  Bryan Wittmann ,  Matthew Mitchell

IPC分类号： G01N30/88 ,  H01J49/00 ,  G01N30/72 ,  G01N33/487

CPC分类号： G01N30/88 ,  G01N30/7206 ,  G01N30/7233 ,  G01N33/487 ,  G01N33/50 ,  G01N2030/8813 ,  G01N2800/00 ,  G01N2800/04 ,  G01N2800/52 ,  H01J49/0036

摘要： Methods are described herein for small molecule biochemical profiling of an individual subject for diagnosis of a disease or disorder, facilitating diagnosis of a disease or disorder, and/or identifying an increased risk of developing a disease or disorder in the individual subject. Aberrant levels of small molecules present in a sample from an individual subject are identified and diagnostic information relevant to the individual subject is obtained based on the identified aberrant levels. The obtained diagnostic information includes one or more of an identification of at least one biochemical pathway associated with the identified subset of the small molecules having aberrant levels, an identification at least one disease or disorder associated with the identified subset of the small molecules having aberrant levels, and an identification of at least one recommended follow up test associated with the identified subset of the small molecules having aberrant levels.

公开(公告)号：US20160356798A1

公开(公告)日：2016-12-08

申请号：US15104560

申请日：2014-12-18

申请人： METABOLON, INC.

发明人： Steven M. WATKINS

IPC分类号： G01N33/92

CPC分类号： G01N33/92 ,  G01N2405/00 ,  G01N2800/7085

摘要： Biomarkers relating to de novo lipogenesis are provided, as well as methods for using such biomarkers in an Index to assess DNL. In addition, methods for diagnosing, determining predisposition to, and monitoring progression/regression of diseases related to DNL are provided. Also provided are methods of monitoring the efficacy of treatments for diseases related to DNL as well as other methods based on biomarkers of DNL.

摘要翻译： 提供了与新生脂肪生成相关的生物标志物，以及在索引中使用这些生物标志物评估DNL的方法。 此外，还提供了诊断，确定与DNL相关疾病的倾向和监测进展/退化的方法。 还提供了监测与DNL相关疾病的治疗功效以及基于DNL生物标志物的其它方法的方法。

公开(公告)号：US20160116486A1

公开(公告)日：2016-04-28

申请号：US14889930

申请日：2014-05-13

申请人： METABOLON, INC.

发明人： Regis PERICHON ,  Jeffrey Edmond COBB ,  Meredith V. BROWN ,  Adam KENNEDY

IPC分类号： G01N33/68 ,  G01N33/70 ,  G01N33/64

摘要： Biomarkers of kidney function and methods for using said biomarkers for assessing kidney function, monitoring kidney function, diagnosing acute kidney injury, and diagnosing chronic kidney disease are provided. Also provided are suites of small molecule entities as biomarkers for chronic kidney disease.

摘要翻译： 提供了肾脏功能的生物标志物和使用所述生物标志物评估肾功能，监测肾功能，诊断急性肾损伤和诊断慢性肾脏疾病的方法。 还提供了小分子实体的套件作为慢性肾脏疾病的生物标志物。

公开(公告)号：US20160116461A1

公开(公告)日：2016-04-28

申请号：US14948457

申请日：2015-11-23

申请人： Metabolon, Inc.

发明人： John RYALS ,  Mike MILBURN ,  Matthew W. MITCHELL ,  Kay A. LAWTON

IPC分类号： G01N33/50 ,  G01N30/72

CPC分类号： G01N33/5091 ,  C12Q1/6876 ,  C12Q1/6883 ,  G01N30/7233 ,  G01N33/5023 ,  G01N2800/7042

摘要： Biomarkers relating to metabolic age are provided, as well as methods for using such biomarkers as biomarkers for determining metabolic age. In addition, methods for modulating the metabolic age of a subject are also provided. Also provided are suites of small molecule entities as biomarkers for metabolic age.

摘要翻译： 提供与代谢年龄相关的生物标志物，以及使用这些生物标志物作为生物标志物来确定代谢年龄的方法。 此外，还提供了调节受试者的代谢年龄的方法。 还提供了小分子实体的套件作为代谢年龄的生物标志物。

公开(公告)号：US20160019335A1

公开(公告)日：2016-01-21

申请号：US14856184

申请日：2015-09-16

申请人： METABOLON, INC.

发明人： Corey Donald DeHaven ,  Robnet Thornhill Kerns

IPC分类号： G06F19/12 ,  G06F19/24 ,  G06F19/26

CPC分类号： G16B45/00 ,  G16B5/00 ,  G16B40/00

摘要： A method is provided for analyzing metabolite data in a sample, comprising analyzing a sample to determine a first number of metabolites, and amount of each metabolite, included in the sample, and a second number of the first number of metabolites that are regulated. Biochemical pathways are determined, each having a third number of the first number of metabolites that are included in the sample and in the determined biochemical pathway. For each of the determined biochemical pathways having the third number of metabolites, a fourth number of the second number of metabolites that are included in the sample and in the determined biochemical pathway that are regulated metabolites is determined. Each of the third number of metabolites within one of the determined biochemical pathways is displayed and distinguished by the amount of each corresponding metabolite included in the sample. An associated apparatus and computer program product are also provided.

摘要翻译： 提供了一种用于分析样品中的代谢物数据的方法，包括分析样品以确定第一数量的代谢物，以及包含在样品中的每种代谢物的量和被调节的第二数量的代谢物。 确定生物化学途径，每个具有第三数量的第一数量的代谢物，其包括在样品中和在确定的生物化学途径中。 对于具有第三代谢物数量的确定的生物化学途径的每一个，测定包含在样品中的第二数量的代谢物和被调节的代谢物的确定的生物化学途径。 在所确定的生物化学途径之一内的第三代代谢物中的每一种被显示并且通过样品中包含的每个相应代谢物的量来区分。 还提供了相关联的设备和计算机程序产品。