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公开(公告)号：US11661576B2
公开(公告)日：2023-05-30
申请号：US16781723
申请日：2020-02-04
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
CPC classification number: C12M25/14 , C12M23/02 , C12M23/20 , C12M23/44 , C12M25/04 , C12M29/10 , C12M29/26 , C12M41/26 , C12M25/02 , C12N5/0068 , C12N5/0602 , C12N2513/00 , C12N2533/30 , C12N2533/40 , C12N2533/90 , C12N2535/00
Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
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公开(公告)号：US11639489B2
公开(公告)日：2023-05-02
申请号：US17869334
申请日：2022-07-20
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
Abstract: A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.
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公开(公告)号：US11584906B2
公开(公告)日：2023-02-21
申请号：US16628309
申请日：2018-07-13
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Jeffrey Glenn Lynn
Abstract: A cell culture vessel (100) has walls and a substrate having a plurality of microcavities (120), where each microcavity of the plurality of microcavities includes a concave well and an opening to allow the microcavity to be filled with liquid. A flange (170) surrounds the substrate having an array of microcavities. A channel (175, 176) surrounds the flange, providing a moat around the microcavity substrate. The flange is angled. Methods of culturing cells in the cell culture vessel are also provided.
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公开(公告)号：US20220364031A1
公开(公告)日：2022-11-17
申请号：US17869334
申请日：2022-07-20
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
Abstract: A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.
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公开(公告)号：US20220081661A1
公开(公告)日：2022-03-17
申请号：US17532196
申请日：2021-11-22
Applicant: CORNING INCORPORATED
Inventor： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Allison Jean Tanner , Qi Wu
Abstract: A cell culture apparatus may include a substrate defining a well. The well may define an interior surface, an exterior surface, an upper aperture and a nadir. The substrate may define a thickness between the interior and exterior surfaces that has a thickness proximate the nadir that is greater than or equal to a thickness proximate the upper aperture.
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Patent Agency Ranking
公开(公告)号：US20220062186A1
公开(公告)日：2022-03-03
申请号：US17501325
申请日：2021-10-14
Applicant: CORNING INCORPORATED
Inventor： Ronald Allen Faris , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Jin Liu , Gregory Roger Martin
IPC: A61K9/28
Abstract: An ex vivo cell culture sustained release composition, including: a mixture comprising: a sustenant; and a non-biodegradable binder; and a non-biodegradable and water insoluble encapsulant coat that encapsulates the mixture, as defined herein. Also disclosed is a method for sustainably providing a sustenant to a cell culture in aqueous media, including contacting a cell culture with the sustained release composition.
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公开(公告)号：US20210371791A1
公开(公告)日：2021-12-02
申请号：US17402078
申请日：2021-08-13
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
Abstract: A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.
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公开(公告)号：US20210371789A1
公开(公告)日：2021-12-02
申请号：US17402047
申请日：2021-08-13
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
Abstract: A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.
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公开(公告)号：US20210130761A1
公开(公告)日：2021-05-06
申请号：US17039035
申请日：2020-09-30
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
Abstract: A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.
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公开(公告)号：US20210062142A1
公开(公告)日：2021-03-04
申请号：US17022764
申请日：2020-09-16
Applicant: CORNING INCORPORATED
Inventor： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , David Henry , Martial Hervy , Corinne Walerack , Yue Zhou
IPC: C12N5/00 , C08J3/24 , C09D167/00
Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.
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