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45 条记录 (耗时 0.029 秒)

    Alphavirus RNA replicon systems
    41.
    发明授权
    Alphavirus RNA replicon systems 有权
    甲病毒RNA复制子系统

    公开(公告)号：US07235235B2

    公开(公告)日：2007-06-26

    申请号：US10388327

    申请日：2003-03-13

    申请人： Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    发明人： Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    IPC分类号： A01N63/00 ,  A61K39/12 ,  C12N7/00 ,  C12N7/04 ,  C12N7/02 ,  C12N5/00

    CPC分类号： C07K14/005 ,  A61K2039/5254 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2770/36162 ,  C12N2840/20

    摘要： The present invention provides a helper cell for expressing an infectious, replication defective, alphavirus particle in an alphavirus-permissive cell. The helper cell includes (a) a first helper RNA encoding (i) at least one alphavirus structural protein, and (ii) not encoding at least one alphavirus structural protein; and (b) a second helper RNA separate from the first helper RNA, the second helper RNA (i) not encoding the alphavirus structural protein encoded by the first helper RNA, and (ii) encoding the at least alphavirus one structural protein not encoded by the first helper RNA, such that all of the alphavirus structural proteins assemble together into alphavirus particles in the cell. Preferably, the helper cell also includes a replicon RNA encoding an alphavirus packaging sequence and an inserted heterogeneous RNA.

    摘要翻译： 本发明提供了用于在甲病毒允许细胞中表达感染性，复制缺陷型甲病毒颗粒的辅助细胞。 辅助细胞包括（a）编码（i）至少一种甲病毒结构蛋白的第一辅助RNA，和（ii）不编码至少一种甲病毒属结构蛋白; 和（b）与第一辅助RNA分开的第二辅助RNA，不编码由第一辅助RNA编码的甲病毒结构蛋白的第二辅助RNA（i），和（ii）编码至少一种不是由第一辅助RNA编码的结合蛋白的结构蛋白， 第一辅助RNA，使得所有甲病毒结构蛋白聚集在细胞中的甲病毒颗粒中。 优选地，辅助细胞还包括编码甲病毒包装序列和插入的异质RNA的复制子RNA。

    Functional influenza virus-like particles (VLPs)
    42.
    发明申请
    Functional influenza virus-like particles (VLPs) 有权

    公开(公告)号：US20060263804A1

    公开(公告)日：2006-11-23

    申请号：US11372466

    申请日：2006-03-10

    申请人： Robin Robinson ,  Peter Pushko

    发明人： Robin Robinson ,  Peter Pushko

    IPC分类号： C12Q1/68 ,  C12P21/06

    CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/575 ,  A61K2039/70 ,  C07H21/04 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/16023 ,  C12N2760/16034 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134

    摘要： Recombinant influenza virus proteins, including influenza capsomers, subviral particles, virus-like particles (VLP), VLP complexes, and/or any portions of thereof, are provided as a vaccine for influenza viruses. The invention is based on the combination of two vaccine technologies: (1) intrinsically safe recombinant vaccine technology, and (2) highly immunogenic, self-assembled protein macromolecules embedded in plasma membranes and comprised of multiple copies of influenza virus structural proteins exhibiting neutralizing epitopes in native conformations. More specifically, this invention relates to the design and production of functional homotypic and heterotypic recombinant influenza virus-like particles (VLPs) comprised of recombinant structural proteins of human influenza virus type A/Sydney/5/94 (H3N2) and/or avian influenza virus type A/Hong Kong/1073/99 (H9N2) in baculovirus-infected insect cells and their application as a vaccine in the prevention of influenza infections and as a laboratory reagent for virus structural studies and clinical diagnostics.

    Alphavirus RNA replicon systems
    44.
    发明授权
    Alphavirus RNA replicon systems 有权
    甲病毒RNA复制子系统

    公开(公告)号：US06521235B2

    公开(公告)日：2003-02-18

    申请号：US09803600

    申请日：2001-03-09

    申请人： Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    发明人： Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    IPC分类号： A61K3912

    CPC分类号： C07K14/005 ,  A61K2039/5254 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2770/36162 ,  C12N2840/20

    摘要： The present invention provides a helper cell for expressing an infectious, replication defective, alphavirus particle in an alphavirus-permissive cell. The helper cell includes (a) a first helper RNA encoding (i) at least one alphavirus structural protein, and (ii) not encoding at least one alphavirus structural protein; and (b) a second helper RNA separate from the first helper RNA, the second helper RNA (i) not encoding the alphavirus structural protein encoded by the first helper RNA, and (ii) encoding the at least one alphavirus structural protein not encoded by the first helper RNA. Preferably, the helper cell is co-transfected with a replicon RNA encoding an alphavirus packaging segment and an inserted heterogeneous RNA, such that all of the alphavirus structural proteins assemble together into alphavirus particles in the cell, with said replicon RNA packaged therein.

    摘要翻译： 本发明提供了用于在甲病毒允许细胞中表达感染性，复制缺陷型甲病毒颗粒的辅助细胞。 辅助细胞包括（a）编码（i）至少一种甲病毒结构蛋白的第一辅助RNA，和（ii）不编码至少一种甲病毒属结构蛋白; 和（b）与第一辅助RNA分开的第二辅助RNA，不编码由第一辅助RNA编码的甲病毒结构蛋白的第二辅助RNA（i），和（ii）编码不由第一辅助RNA编码的至少一种甲病毒结构蛋白， 第一个辅助RNA。 优选地，辅助细胞与编码甲病毒包装片段和插入的异质RNA的复制子RNA共转染，使得所有甲病毒属结构蛋白在一起组装成细胞中的甲病毒颗粒，其中包含所述复制子RNA。