公开(公告)号：US11123733B2

公开(公告)日：2021-09-21

申请号：US16691405

申请日：2019-11-21

Applicant: Illumina, Inc.

Inventor： Hamed Amini ,  Arash Jamshidi ,  Tarun Kumar Khurana ,  Foad Mashayekhi ,  Yir-Shyuan Wu

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00

Abstract: Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.

公开(公告)号：US10953399B2

公开(公告)日：2021-03-23

申请号：US16244776

申请日：2019-01-10

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Wesley A. Cox-Muranami ,  Darren Robert Segale ,  Oliver Jon Miller ,  Jennifer Olivia Foley ,  Tarun Kumar Khurana ,  Paul Crivelli ,  Gary Watts

IPC: G01N1/31 ,  B01L3/00 ,  B01L9/00

Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：US20200238276A1

公开(公告)日：2020-07-30

申请号：US16750930

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Arnaud Rival ,  Lewis J. Kraft ,  Steven Barnard ,  M. Shane Bowen ,  Xi-Jun Chen ,  Yir-Shyuan Wu ,  Jeffrey S. Fisher ,  Dajun Yuan

IPC: B01L3/00 ,  C12N15/10

Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

公开(公告)号：US20200238247A1

公开(公告)日：2020-07-30

申请号：US16750831

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/18 ,  B01L3/00

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.