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1.发明申请公开(公告)号：US20160199832A1
公开(公告)日：2016-07-14
申请号：US14912394
申请日：2014-08-29
发明人： Arash Jamshidi , Yan-You Lin , Alex Aravanis , Cyril Delattre , Arnaud Rival , Jennifer Foley , Poorya Sabounchi , Tarun Khurana , Majid Babazadeh , Hamed Amini , Bala Murali Venkatesan , M. Shane Bowen , Steven M. Barnard , Maria Candelaria Rogert Bacigalupo , Dietrich Dehlinger
IPC分类号： B01L3/00
CPC分类号： B01L3/50273 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/502792 , B01L2200/06 , B01L2300/0636 , B01L2300/0819 , B01L2300/0858 , B01L2300/087 , B01L2300/161 , B01L2300/166 , B01L2400/0427 , B41J2/04 , G01N15/1463 , G01N15/1475 , G01N2015/1006
摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.
摘要翻译： 本文提供的液滴致动器包括（a）由液滴操作间隙分离的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到第一衬底和第二衬底中的至少一个的多个电极，电极沿着液滴操作间隙排列，以控制液滴沿着液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。
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公开(公告)号：US09309571B2
公开(公告)日：2016-04-12
申请号：US14084355
申请日：2013-11-19
申请人： ILLUMINA, INC.
发明人： Min-Jui Richard Shen , Robert C. Kain , Kenneth M. Kuhn , AmirAli Hajhossein Talasaz , Arash Jamshidi , George Sakaldasis , Eric Vermaas , Sebastian Bohm , Tarun Khurana , Helmy A. Eltoukhy , Jian Gong
CPC分类号： C12Q1/6874 , B01L3/502792 , B01L2200/0673 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0851 , B01L2300/165 , B01L2400/0427
摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.
摘要翻译： 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在同一装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包括用于样品制备和/或测序的试剂的预装的消耗品筒。
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公开(公告)号：US20140073514A1
公开(公告)日：2014-03-13
申请号：US14084355
申请日：2013-11-19
申请人： ILLUMINA, INC.
发明人： Min-Jui Richard Shen , Robert C. Kain , Kenneth M. Kuhn , AmirAli Hajhossein Talasaz , Arash Jamshidi , George Sakaldasis , Eric Vermaas , Sebastian Bohm , Tarun Khurana , Helmy A. Eltoukhy , Jian Gong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , B01L3/502792 , B01L2200/0673 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0851 , B01L2300/165 , B01L2400/0427
摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.
摘要翻译： 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在同一装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包括用于样品制备和/或测序的试剂的预装的消耗品筒。
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公开(公告)号：US20130116128A1
公开(公告)日：2013-05-09
申请号：US13670318
申请日：2012-11-06
申请人： ILLUMINA, INC.
发明人： Min-Jui Richard Shen , Robert C. Kain , Kenneth M. Kuhn , AmirAli Hajhossein Talasaz , Arash Jamshidi , George Sakaldasis , Eric Vermaas , Sebastian Bohm , Tarun Khurana , Helmy A. Eltoukhy , Jian Gong
CPC分类号： C12Q1/6874 , B01L3/502792 , B01L2200/0673 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0851 , B01L2300/165 , B01L2400/0427
摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.
摘要翻译： 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在同一装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包括用于样品制备和/或测序的试剂的预装的消耗品筒。
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公开(公告)号：US10975371B2
公开(公告)日：2021-04-13
申请号：US14855207
申请日：2015-09-15
申请人： ILLUMINA, INC.
发明人： Neeraj Salathia , Jian-Bing Fan , Fiona Kaper , Gordon M. Cann , Arash Jamshidi , Alex Aravanis
IPC分类号： C12N15/10 , C12Q1/6869 , C12Q1/6853 , C12Q1/6806
摘要： Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).
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6.发明申请公开(公告)号：US20180250672A1
公开(公告)日：2018-09-06
申请号：US15779459
申请日：2016-11-30
申请人： Illumina, Inc.
发明人： Arash Jamshidi , Yan-you Lin , Farnaz Absalan , Sarah Stuart , Gordon Cann , Yir-Shyuan Wu , Tarun Khurana , Jeffrey S Fisher
IPC分类号： B01L3/00 , C12Q1/6806
CPC分类号： B01L3/502761 , B01L3/502784 , B01L3/5088 , B01L2200/0605 , B01L2200/0642 , B01L2200/0668 , B01L2300/0819 , B01L2300/165 , B01L2400/0427 , B01L2400/043 , C12Q1/6806 , C12Q1/6869 , C12Q2563/143 , C12Q2563/149 , C12Q2563/185 , G01N1/28 , G01N33/54333 , G01N2015/1006 , C12Q2535/122
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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7.发明公开公开(公告)号：US20240299944A1
公开(公告)日：2024-09-12
申请号：US18664102
申请日：2024-05-14
申请人： Illumina, Inc.
发明人： Arash Jamshidi , Yan-you Lin , Farnaz Absalan , Sarah Stuart , Gordon Cann , Yir-Shyuan Wu , Tarun Khurana , Jeffrey S Fisher
IPC分类号： B01L3/00 , C12Q1/6806 , C12Q1/6869 , G01N1/28 , G01N15/10 , G01N33/543
CPC分类号： B01L3/502761 , B01L3/502784 , B01L3/5088 , C12Q1/6806 , G01N1/28 , G01N33/54333 , B01L2200/0605 , B01L2200/0642 , B01L2200/0668 , B01L2300/0819 , B01L2300/165 , B01L2400/0427 , B01L2400/043 , C12Q1/6869 , C12Q2563/143 , C12Q2563/149 , C12Q2563/185 , G01N2015/1006
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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公开(公告)号：US11203016B2
公开(公告)日：2021-12-21
申请号：US15779459
申请日：2016-11-30
申请人： Illumina, Inc.
发明人： Arash Jamshidi , Yan-you Lin , Farnaz Absalan , Sarah Stuart , Gordon Cann , Yir-Shyuan Wu , Tarun Khurana , Jeffrey S Fisher
IPC分类号： B01L3/00 , C12Q1/6806 , G01N1/28 , G01N33/543 , C12Q1/6869 , G01N15/10
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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公开(公告)号：US11123733B2
公开(公告)日：2021-09-21
申请号：US16691405
申请日：2019-11-21
申请人： Illumina, Inc.
摘要： Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.
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公开(公告)号：US10167505B2
公开(公告)日：2019-01-01
申请号：US15067013
申请日：2016-03-10
申请人： Illumina, Inc.
发明人： Min-Jui Richard Shen , Robert C. Kain , Kenneth M. Kuhn , AmirAli Hajhossein Talasaz , Arash Jamshidi , George Sakaldasis , Eric Vermaas , Sebastian Bohm , Tarun Khurana , Helmy A. Eltoukhy , Jian Gong
IPC分类号： C12Q1/68 , B01L3/00 , C12Q1/6874 , B81B7/00
摘要： Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.
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