公开(公告)号：US08262990B2

公开(公告)日：2012-09-11

申请号：US12857405

申请日：2010-08-16

Applicant: Nathaniel C. Bair ,  Collin A. Rich

Inventor： Nathaniel C. Bair ,  Collin A. Rich

IPC: G01N21/00 ,  B08B7/00 ,  F17D1/00 ,  G01N35/08

CPC classification number: F04B23/04 ,  F04B43/0081 ,  F04B43/12 ,  F04B2201/1201 ,  F04B2205/09 ,  G01N15/1404 ,  G01N2015/1413 ,  G05D7/0682 ,  Y10T137/0352 ,  Y10T137/0379 ,  Y10T137/0402 ,  Y10T137/85986 ,  Y10T436/115831 ,  Y10T436/117497

Abstract: The fluidic system with an unclogging feature of the preferred embodiment includes a flow channel, a sheath pump to pump sheath fluid from a sheath container into an interrogation zone, and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The pump and controller cooperate to propagate a pulsation through the flow channel from the pump if the flow channel is clogged. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.

Abstract translation: 具有优选实施例的不记录特征的流体系统包括流动通道，用于将护套流体从鞘容器泵送到询问区域的护套泵以及将废液从询问区泵送到废物容器中的废水泵。 护套泵和/或废水泵将样品流体从样品容器中抽入询问区。 流体系统还包括控制器，用于调节样品流体从样品容器进入询问区域的流速。 如果流道被堵塞，泵和控制器协同传播来自泵的流动通道的脉动。 流体系统优选地结合到具有包括询问区的流动池的流式细胞仪中。

公开(公告)号：US07981661B2

公开(公告)日：2011-07-19

申请号：US11736412

申请日：2007-04-17

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: C12M1/36 ,  G01N33/48 ,  G01N27/22

CPC classification number: G01N15/1404 ,  G01N2015/1409 ,  Y10T436/115831

Abstract: The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone, a sheath volume measurement device to measure the fluid in the sheath container, a waste pump to pump the sheath fluid and a sample fluid as waste fluid from the interrogation zone into a waste container, and a waste volume measurement device to measure the fluid in the waste container. The system also includes a controller connected to the sheath pump, the waste pump, and the volume measurement devices. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone, which functions to provide a location for the fluidic system and an optical system of the flow cytometer to cooperatively facilitate the analysis of the sample fluid.

Abstract translation: 优选实施例的流体系统包括鞘套泵，以将护套流体从护套容器泵送到询问区域中;护套体积测量装置，用于测量护套容器中的流体;泵送护套流体的废泵和样品流体 作为从询问区到废物容器的废液，以及用于测量废物容器中的流体的废物体积测量装置。 该系统还包括连接到护套泵，废水泵和体积测量装置的控制器。 护套泵和/或废液泵将样品流体从样品容器抽入询问区，其功能是提供流体系统的位置和流式细胞计数器的光学系统，以便协调地促进样品流体的分析。

公开(公告)号：US20110058163A1

公开(公告)日：2011-03-10

申请号：US12939836

申请日：2010-11-04

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: G01N1/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G02B27/141 ,  G02B27/145

Abstract: The optical system of the preferred embodiments includes a first light source that creates a first beam of a first wavelength, a first collimating element that collimates the first beam, a second light source 102 that creates a second beam of a second wavelength, a second collimating element that collimates the second beam, a beam combining element that combines the collimated beams, and a focusing element that focuses the combined collimated beam to a single point.

Abstract translation: 优选实施例的光学系统包括产生第一波长的第一光束的第一光源，准直第一光束的第一准直元件，产生第二波长的第二光束的第二光源102，第二准直 对第二光束进行准直的元件，组合准直光束的光束组合元件以及将组合的准直光束聚焦到单个点的聚焦元件。

公开(公告)号：US07780916B2

公开(公告)日：2010-08-24

申请号：US11735456

申请日：2007-04-14

Applicant: Nathaniel C. Bair ,  Collin A. Rich

Inventor： Nathaniel C. Bair ,  Collin A. Rich

IPC: G01N21/00 ,  E03B1/00 ,  B01L3/02 ,  G01N15/06 ,  G01N35/02

CPC classification number: F04B23/04 ,  F04B43/0081 ,  F04B43/12 ,  F04B2201/1201 ,  F04B2205/09 ,  G01N15/1404 ,  G01N2015/1413 ,  G05D7/0682 ,  Y10T137/0352 ,  Y10T137/0379 ,  Y10T137/0402 ,  Y10T137/85986 ,  Y10T436/115831 ,  Y10T436/117497

Abstract: The fluidic system with an unclogging feature of the preferred embodiment includes a flow channel, a sheath pump to pump sheath fluid from a sheath container into an interrogation zone, and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The pump and controller cooperate to propagate a pulsation through the flow channel from the pump if the flow channel is clogged. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.

Abstract translation: 具有优选实施例的不记录特征的流体系统包括流动通道，用于将护套流体从鞘容器泵送到询问区域的护套泵以及将废液从询问区泵送到废物容器中的废水泵。 护套泵和/或废水泵将样品流体从样品容器中抽入询问区。 流体系统还包括控制器，用于调节样品流体从样品容器进入询问区域的流速。 如果流道被堵塞，泵和控制器协同传播来自泵的流动通道的脉动。 流体系统优选地结合到具有包括询问区的流动池的流式细胞仪中。

公开(公告)号：US20100118298A1

公开(公告)日：2010-05-13

申请号：US12514052

申请日：2007-11-07

Applicant: Nathaniel C. Bair ,  Collin A. Rich ,  Mark Robert Eadie ,  Rebecca Ann Lehrmann

Inventor： Nathaniel C. Bair ,  Collin A. Rich ,  Mark Robert Eadie ,  Rebecca Ann Lehrmann

IPC: G01N1/10 ,  B23P11/00

CPC classification number: G01N15/1404 ,  G01N2015/1413 ,  Y10T29/49826 ,  Y10T137/0352 ,  Y10T137/0379 ,  Y10T137/0419 ,  Y10T436/115831 ,  Y10T436/117497

Abstract: The flow cytometer system of the preferred embodiment includes a flow cell body that functions to contain, protect, and align the components of the flow cytometer system; a flow channel, coupled to the flow cell body, that functions to conduct and focus sample fluid through an interrogation zone; and a sample injection probe, removably coupled to the flow cell body, that functions to provide a uniform flow of sample fluid to the flow channel. The flow cytometer system is preferably designed for the flow cytometer field. The flow cytometer system, however, may be alternatively used in any suitable environment and for any suitable reason.

Abstract translation: 优选实施例的流式细胞仪系统包括流动池主体，其用于容纳，保护和对准流式细胞仪系统的部件; 耦合到流动池主体的流动通道，其用于将样品流体引导和聚焦通过询问区; 以及可移除地耦合到流动池主体的样​​品注入探针，其用于提供样品流体到流动通道的均匀流动。 流式细胞仪系统优选设计用于流式细胞仪领域。 然而，流式细胞仪系统可以替代地在任何合适的环境中用于任何合适的原因。

公开(公告)号：US07520300B2

公开(公告)日：2009-04-21

申请号：US11958278

申请日：2007-12-17

Applicant: Collin A. Rich ,  Steven M. Martin

Inventor： Collin A. Rich ,  Steven M. Martin

IPC: F16L55/04

CPC classification number: F15B1/021 ,  F04B43/12 ,  F15B1/10 ,  F15B21/008 ,  F15B2201/205 ,  F15B2201/215 ,  F15B2201/3151 ,  G01N15/1404 ,  Y10T137/3118 ,  Y10T137/7792

Abstract: A pulsation attenuator for a fluidic system with a fluidic pump. The pulsation attenuator includes a fluidic channel, a first fluidic device adapted to attenuate pulsations with a shallow rolloff slope, and a second fluidic device adapted to attenuate pulsations with a shallow rolloff slope. The first fluidic device and the second fluidic device are connected to the fluidic channel such that they cooperatively attenuate pulsations with a steep rolloff slope. Preferably, the first fluidic device includes a first fluidic resistor and a first fluidic capacitor, and the second fluidic device includes a second fluidic resistor and a second fluidic capacitor. Preferably, the pulsation attenuator is arranged, similar a second-order low-pass filter, in the following order: (1) first fluidic resistor, (2) first fluidic capacitor, (3) second fluidic resistor, and (4) second fluidic capacitor.

Abstract translation: 一种具有流体泵的流体系统的脉动衰减器。 脉动衰减器包括流体通道，适于衰减具有浅滚降斜率的脉动的第一流体装置，以及适于以浅滚降斜率衰减脉动的第二流体装置。 第一流体装置和第二流体装置连接到流体通道，使得它们以陡峭的滚降斜率协同地衰减脉动。 优选地，第一流体装置包括第一流体电阻器和第一流体电容器，并且第二流体装置包括第二流体电阻器和第二流体电容器。 优选地，按照以下顺序将脉动衰减器类似于二阶低通滤波器：（1）第一流体电阻器，（2）第一流体电容器，（3）第二流体电阻器，和（4）第二流体电阻器 电容器。

公开(公告)号：US20080228444A1

公开(公告)日：2008-09-18

申请号：US11466391

申请日：2006-08-22

Applicant: David Olson ,  Collin A. Rich ,  Clement James Goebel

Inventor： David Olson ,  Collin A. Rich ,  Clement James Goebel

IPC: G06F15/00

CPC classification number: G01N15/1429 ,  G01N35/00712 ,  G01N35/0092

Abstract: The flow cytometer user interface of the preferred embodiment comprises the steps of (1) running the sample and saving all collected data, (2) viewing the raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified settings, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified settings, and exporting the saved data as many times as necessary and/or desirable without the need to run an additional sample.

Abstract translation: 优选实施例的流式细胞仪用户界面包括以下步骤：（1）运行样本并保存所有收集的数据，（2）查看原始（或“未修改”）数据，（3）修改原始数据（例如缩放 和/或剔除原始数据），（4）查看和保存修改的设置，以及（5）导出保存的数据。 一旦样品运行并且所有收集的数据都被保存，用户可以重复修改原始数据，保存修改后的设置以及根据需要和/或需要导出保存的数据的步骤，而不需要运行 额外的样品。

公开(公告)号：US20080215297A1

公开(公告)日：2008-09-04

申请号：US11963384

申请日：2007-12-21

Applicant: Clement James Goebel ,  Collin A. Rich

Inventor： Clement James Goebel ,  Collin A. Rich

IPC: G06F15/00 ,  G01N21/00

CPC classification number: G01N15/1459 ,  G01N2015/0092 ,  G01N2015/1477

Abstract: The detection system of the first preferred embodiment includes a detector, having a wide dynamic range, that receives photonic inputs from the interrogation zone and produces an analog signal; and an analog-to-digital converter (ADC), having a high bit resolution, that is coupled to the detector and converts an analog signal to a digital signal. The digital signal includes an initial data set of the full dynamic range of the input signals from the flow cytometer sample. The method of extracting and analyzing data from a flow cytometer system of the first preferred embodiment preferably includes the steps of: collecting a full dynamic range of input signals from a flow cytometer sample; recognizing and annotating aggregate particle events; and storing an initial data set and an annotated data set of the full dynamic range of the input signals from the flow cytometer sample.

Abstract translation: 第一优选实施例的检测系统包括具有宽动态范围的检测器，其接收来自询问区的光子输入并产生模拟信号; 以及具有高比特分辨率的模数转换器（ADC），其耦合到检测器并将模拟信号转换为数字信号。 数字信号包括来自流式细胞仪样本的输入信号的完整动态范围的初始数据集。 从第一优选实施例的流式细胞仪系统中提取和分析数据的方法优选地包括以下步骤：从流式细胞仪样品收集输入信号的完整动态范围; 识别和注释聚集粒子事件; 并存储来自流式细胞仪样本的输入信号的完整动态范围的初始数据集和注释数据集。

公开(公告)号：US20130204538A1

公开(公告)日：2013-08-08

申请号：US13820968

申请日：2011-10-25

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: G06F19/10

CPC classification number: G01N15/1434 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/18 ,  G01J3/36 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1429 ,  G06F19/10 ,  G06F19/12 ,  G06F19/26 ,  G06M11/02

Abstract: Systems in a flow cytometer having an interrogation zone and illumination impinging the interrogation zone include: a lens subsystem including a collimating element that collimates light from the interrogation zone, a light dispersion element that disperses collimated light into a light spectrum, and a focusing lens that focuses the light spectrum onto an array of adjacent detection points; a detector array, including semiconductor detector devices, that collectively detects a full spectral range of input light signals, in which each detector device detects a subset spectral range of the full spectral range of light signals; and a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and has a virtual detector channel range including the sum of subset spectral ranges of the detectors in the corresponding detector group.

Abstract translation: 具有询问区和照射询问区的照明的流式细胞仪中的系统包括：透镜子系统，包括准直来自询问区的光的准直元件，将准直光分散到光谱中的光分散元件和聚焦透镜， 将光谱聚焦到相邻检测点的阵列上; 检测器阵列，包括半导体检测器装置，其共同地检测输入光信号的全光谱范围，其中每个检测器装置检测光信号的全光谱范围的子集光谱范围; 以及用户界面，其使得用户能够通过对检测器阵列中的检测器进行分组来创建一组虚拟检测器通道，使得每个虚拟检测器通道对应于检测器组并且具有虚拟检测器通道范围，该虚拟检测器通道范围包括 相应检测器组中的检测器。

公开(公告)号：US08149402B2

公开(公告)日：2012-04-03

申请号：US12197192

申请日：2008-08-22

Applicant: Collin A. Rich ,  Richard L. Fisher ,  Nathaniel C. Bair

Inventor： Collin A. Rich ,  Richard L. Fisher ,  Nathaniel C. Bair

IPC: G01N21/00

CPC classification number: G01N21/53 ,  G01N15/1434 ,  G01N2021/6497 ,  Y10T436/115831

Abstract: The preferred embodiments of the invention is an optical system for a flow cytometer including a flow channel with an interrogation zone, and an illumination source that impinges the flow channel in the interrogation zone from a particular direction. The optical system preferably includes a lens system and a detection system. The lens system preferably includes multiple lens surfaces arranged around the flow channel and adapted to collect and collimate light from the interrogation zone. The detection system preferably includes multiple detectors adapted to detect light from the lens system. Each detector preferably includes a local filter that independently filters for specific wavelengths. Thus, the user may easily swap the filters in any order to achieve the same detection parameters.

Abstract translation: 本发明的优选实施例是用于流式细胞仪的光学系统，其包括具有询问区的流动通道，以及照射源，其将特定方向上的询问区中的流动通道入射。 光学系统优选地包括透镜系统和检测系统。 透镜系统优选地包括围绕流动通道布置的多个透镜表面，并且适于收集和准直来自询问区域的光。 检测系统优选地包括适于检测来自透镜系统的光的多个检测器。 每个检测器优选地包括独立地滤波特定波长的局部滤波器。 因此，用户可以以任何顺序容易地交换滤波器以实现相同的检测参数。