利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

46 条记录 (耗时 0.036 秒)

    Method of providing a hybrid polypeptide exhibiting an activity of interest
    41.
    发明授权
    Method of providing a hybrid polypeptide exhibiting an activity of interest 失效
    提供表现出感兴趣活性的杂合多肽的方法

    公开(公告)号：US06270968B1

    公开(公告)日：2001-08-07

    申请号：US09189060

    申请日：1998-11-05

    申请人： Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

    发明人： Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

    IPC分类号： C12Q168

    CPC分类号： C12N15/1044 ,  C12N9/2437 ,  C12N9/248 ,  C12Q1/6811 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01032

    摘要： A method for providing a hybrid polypeptide having an activity of interest, by i) performing PCR amplification using an uncharacterized DNA sample and oligonucleotide primers with homology to one or more known genes encoding a polypeptide exhibiting said activity of interest, to obtain one or more PCR products, ii) linking the obtained PCR products to a 5′ structural gene sequence and a 3′ structural gene sequence, wherein the 5′ and 3′ structural gene sequences are derived from one or more genes encoding a polypeptide exhibiting said activity of interest, to form hybrid DNA sequences, iii) expressing the resulting hybrid DNA sequences, and iv) screening the hybrid polypeptides to identify a sequence encoding a polypeptide exhibiting said activity of interest or a related activity.

    摘要翻译： 一种用于提供具有感兴趣活性的杂交多肽的方法，其通过以下步骤：i）使用未表征的DNA样品进行PCR扩增和与编码显示所述目的活性的多肽的一种或多种已知基因同源的寡核苷酸引物，以获得一种或多种PCR 产品，ii）将获得的PCR产物连接到5'结构基因序列和3'结构基因序列，其中5'和3'结构基因序列衍生自编码显示所述目标活性的多肽的一个或多个基因， 以形成杂交DNA序列，iii）表达所得的杂交DNA序列，和iv）筛选杂合多肽以鉴定编码显示所述目的活性或相关活性的多肽的序列。