利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

46 条记录 (耗时 0.038 秒)

    Method of providing a hybrid polypeptide exhibiting an activity of interest
    1.
    发明授权
    Method of providing a hybrid polypeptide exhibiting an activity of interest 失效
    提供表现出感兴趣活性的杂合多肽的方法

    公开(公告)号：US06270968B1

    公开(公告)日：2001-08-07

    申请号：US09189060

    申请日：1998-11-05

    申请人： Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

    发明人： Henrik Dalbøge ,  Thomas Sandal ,  Markus Sakari Kauppinen ,  Børge Diderichsen

    IPC分类号： C12Q168

    CPC分类号： C12N15/1044 ,  C12N9/2437 ,  C12N9/248 ,  C12Q1/6811 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01032

    摘要： A method for providing a hybrid polypeptide having an activity of interest, by i) performing PCR amplification using an uncharacterized DNA sample and oligonucleotide primers with homology to one or more known genes encoding a polypeptide exhibiting said activity of interest, to obtain one or more PCR products, ii) linking the obtained PCR products to a 5′ structural gene sequence and a 3′ structural gene sequence, wherein the 5′ and 3′ structural gene sequences are derived from one or more genes encoding a polypeptide exhibiting said activity of interest, to form hybrid DNA sequences, iii) expressing the resulting hybrid DNA sequences, and iv) screening the hybrid polypeptides to identify a sequence encoding a polypeptide exhibiting said activity of interest or a related activity.

    摘要翻译： 一种用于提供具有感兴趣活性的杂交多肽的方法，其通过以下步骤：i）使用未表征的DNA样品进行PCR扩增和与编码显示所述目的活性的多肽的一种或多种已知基因同源的寡核苷酸引物，以获得一种或多种PCR 产品，ii）将获得的PCR产物连接到5'结构基因序列和3'结构基因序列，其中5'和3'结构基因序列衍生自编码显示所述目标活性的多肽的一个或多个基因， 以形成杂交DNA序列，iii）表达所得的杂交DNA序列，和iv）筛选杂合多肽以鉴定编码显示所述目的活性或相关活性的多肽的序列。

    Transglutaminase from oomycetes
    3.
    发明授权
    Transglutaminase from oomycetes 失效
    来自卵菌的转谷氨酰胺酶

    公开(公告)号：US06428993B1

    公开(公告)日：2002-08-06

    申请号：US08881742

    申请日：1997-06-24

    申请人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    发明人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    IPC分类号： C12N910

    CPC分类号： C12Y203/02013 ,  A21D8/042 ,  C12N9/1044 ,  C14C11/00 ,  Y10S435/911

    摘要： The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.

    摘要翻译： 本发明涉及从属于卵菌纲的较低真菌获得的转谷氨酰胺酶和转谷氨酰胺酶制剂。 通过生长这些细胞形成的细菌，特别是菌株腐霉菌，不锈钢腐霉，腐霉腐霉（P. periplocum），腐皮腐，腐霉，腐霉，腐皮疫霉，致病疫霉（Phytophthora palmivora），可以实现前所未有的高水平表达 ，疫霉疫霉（Phytophthora porri），致病疫霉（Phytophthora infestans），致病疫霉（Phytoph-thora megasperma），肉桂疫霉（Phytophthora cinnamomi）和疫霉疫霉（Phytophthora cryptogea）。 克隆和表达重组转谷氨酰胺酶，酶和酶制剂可用于交联蛋白质，例如， 在面粉，烘焙食品，肉制品，鱼制品，化妆品，奶酪，乳制品，凝胶食品和皮革整理中，或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。

    Transglutaminase from oomycetes
    4.
    发明授权
    Transglutaminase from oomycetes 失效
    来自卵菌的转谷氨酰胺酶

    公开(公告)号：US07094586B2

    公开(公告)日：2006-08-22

    申请号：US10164765

    申请日：2002-06-07

    申请人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    发明人： Lisbeth Bech ,  Grethe Rasmussen ,  Torben Halkier ,  Mariko Okada ,  Lene Nonboe Andersen ,  Markus Sakari Kauppinen ,  Thomas Sandal

    IPC分类号： C12N9/10 ,  C12N15/54

    CPC分类号： C12Y203/02013 ,  A21D8/042 ,  C12N9/1044 ,  C14C11/00 ,  Y10S435/911

    摘要： The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes and unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytophthora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea; and a recombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.

    摘要翻译： 本发明涉及从属于卵菌纲的低级真菌得到的转谷氨酰胺酶和转谷氨酰胺酶制剂，并且具有前所未有的高水平表达是通过生长这些形成细胞的生物体，特别是菌株腐霉菌，非腐霉菌，不锈菌腐霉，腐霉腐霉（或 P.腐生菌），腐皮腐，腐霉，腐霉，腐皮疫霉，疫霉疫霉，致病疫霉，致热疫霉，肉，疫霉和疫霉疫霉;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;; 并且克隆和表达重组转谷氨酰胺酶，所述酶和酶制剂可用于交联蛋白质，例如， 在面粉，烘焙食品，肉制品，鱼制品，化妆品，奶酪，乳制品，凝胶食品和皮革整理中，或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。

    Enzymes with aminopeptidase activity
    9.
    发明授权
    Enzymes with aminopeptidase activity 失效
    具有氨基肽酶活性的酶

    公开(公告)号：US06413559B1

    公开(公告)日：2002-07-02

    申请号：US09718709

    申请日：2000-11-22

    申请人： Markus Sakari Kauppinen ,  Joan Qi Si ,  Tina Spendler ,  Claus Dambmann ,  Torben Halkier ,  Peter Rahbek Østergaard ,  Shamkant Anant Patkar ,  Kim Hansen

    发明人： Markus Sakari Kauppinen ,  Joan Qi Si ,  Tina Spendler ,  Claus Dambmann ,  Torben Halkier ,  Peter Rahbek Østergaard ,  Shamkant Anant Patkar ,  Kim Hansen

    IPC分类号： A21D200

    CPC分类号： C12N9/48 ,  A21D8/042

    摘要： The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.

    摘要翻译： 本发明涉及一种表现出来自真菌微生物的氨基肽酶活性的35kDa酶，包含编码所述酶的DNA序列的DNA构建体，包含所述DNA构建体的重组表达载体和包含所述DNA序列的细胞。 本发明还提供了生产表现出氨基肽酶活性的酶的方法，以及包含所述酶的酶制剂，包含本发明的氨基肽酶的面包改良组合物或面团改良组合物。 最后，本发明涉及所述显示氨基肽酶活性的酶或酶制剂或其组合物的用途。

    Endoglucanases
    10.
    发明授权
    Endoglucanases 失效
    内切葡聚糖酶

    公开(公告)号：US6001639A

    公开(公告)日：1999-12-14

    申请号：US651136

    申请日：1996-05-21

    申请人： Martin Schulein ,  Lene Nonboe Andersen ,  S.o slashed.ren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Iium Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    发明人： Martin Schulein ,  Lene Nonboe Andersen ,  S.o slashed.ren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Iium Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    IPC分类号： C12N15/09 ,  C05F11/08 ,  C05F17/00 ,  C07G99/00 ,  C11D3/386 ,  C12N1/19 ,  C12N9/42 ,  C12N15/00 ,  C12R1/645 ,  C12R1/69 ,  C12R1/77 ,  C12R1/80 ,  C12S3/04 ,  C12S11/00 ,  D06M13/02 ,  D06M13/322 ,  D06M13/46 ,  D06M16/00 ,  D06P5/02 ,  D06P5/15 ,  D21C5/00 ,  D21H11/16 ,  D21H11/20 ,  D21H17/00 ,  D21H21/10 ,  D21C1/00

    CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H21/10 ,  C07K2319/00 ,  D21H17/005 ,  Y02P20/145 ,  Y02W30/43

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequenceThr Arg Xaa Xaa Asp Cys Cys Xaa Xaa (SEQ ID NO:79) 1 2 3 4 5 6 7 8 9 - Xaa Cys Xaa Trp Xaa 10 11 12 13 14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译： 本发明涉及基本上由具有纤维素分解活性的酶组成的酶制剂，其包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列：Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa（SEQ ID NO：79） - 1 2 3 4 5 6 7 8 9 - - Xaa Cys Xaa Trp Xaa - 10 11 12 13 14 - 和由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列--Trp Cys Cys Xaa Cys（SEQ ID NO ：80） - 1 2 3 4 5 - 其中，在第一序列的位置3，氨基酸是Trp，Tyr或Phe; 在第一序列的位置4，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 分别位于第一序列的第9,10,12和14位，在第二序列的位置4中，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列 ，（i）当位置12的氨基残基为Ser时，14位的氨基酸残基不为Ser，（ii）当位置12的氨基残基为Gly时，位置14的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。