Enzymes with aminopeptidase activity
    3.
    发明授权
    Enzymes with aminopeptidase activity 失效
    具有氨基肽酶活性的酶

    公开(公告)号:US06413559B1

    公开(公告)日:2002-07-02

    申请号:US09718709

    申请日:2000-11-22

    IPC分类号: A21D200

    CPC分类号: C12N9/48 A21D8/042

    摘要: The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.

    摘要翻译: 本发明涉及一种表现出来自真菌微生物的氨基肽酶活性的35kDa酶,包含编码所述酶的DNA序列的DNA构建体,包含所述DNA构建体的重组表达载体和包含所述DNA序列的细胞。 本发明还提供了生产表现出氨基肽酶活性的酶的方法,以及包含所述酶的酶制剂,包含本发明的氨基肽酶的面包改良组合物或面团改良组合物。 最后,本发明涉及所述显示氨基肽酶活性的酶或酶制剂或其组合物的用途。

    Nucleic acid constructs for the production of a Bacillus alkaline
protease
    10.
    发明授权
    Nucleic acid constructs for the production of a Bacillus alkaline protease 失效
    用于生产芽孢杆菌碱性蛋白酶的核酸构建体

    公开(公告)号:US5621089A

    公开(公告)日:1997-04-15

    申请号:US434255

    申请日:1995-05-03

    CPC分类号: C11D3/386 C12N15/75 C12N9/54

    摘要: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.

    摘要翻译: 本发明涉及分离的核酸构建体,其包含编码分子量为约34kD的碱性蛋白酶的核酸序列,并且可从芽孢杆菌属菌株获得。 组I,以及包含这种构建体的重组载体和宿主细胞。 另外,本发明涉及获得蛋白酶的方法。 本发明还涉及来自所述碱性蛋白酶的启动子和信号序列以及在编码细菌中的异源蛋白质的异源核酸序列的表达中使用启动子和信号序列的方法。