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公开(公告)号：US20090095919A1
公开(公告)日：2009-04-16
申请号：US12335380
申请日：2008-12-15
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6417 , G01N2021/6421 , Y10S435/968
摘要： A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.
摘要翻译： 用于监测细胞样本中的细胞活性的系统和方法。 根据一个实施例，将多个可激发标记施加到样本。 提供多光子激光显微镜来激发样品的区域并引起从该区域辐射的荧光。 辐射荧光由光谱分析仪处理，将荧光分离成相应的波段。 然后通过一组检测器收集各个荧光带，每个检测器接收相应的一个波长带。
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公开(公告)号：US20090054267A1
公开(公告)日：2009-02-26
申请号：US12216062
申请日：2008-06-27
IPC分类号： C40B60/12
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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公开(公告)号：US08593638B2
公开(公告)日：2013-11-26
申请号：US12573009
申请日：2009-10-02
申请人： Tao Lu , Tsu-Te Judith Su , Kerry J. Vahala , Scott E. Fraser
发明人： Tao Lu , Tsu-Te Judith Su , Kerry J. Vahala , Scott E. Fraser
IPC分类号： G01B9/02
CPC分类号： G01N21/7746
摘要： Resonant sensors and molecule detection methods utilizing split frequency. Optical energy is introduced into a microcavity, such as a toroid-shaped or spherical microcavity. A portion of the optical energy is backscattered and interacts with the introduced optical energy to form first and second modes of optical energy at respective first and second frequencies, also referred to as split frequency or mode doublets. One or more molecules bind to an outer surface of the microcavity and interact with an evanescent field of optical energy resonating within the microcavity. Binding of one or more molecules to the outer surface is detected based at least in part upon a change of the split frequency relative to a baseline split frequency.
摘要翻译： 谐振传感器和分子检测方法利用分频。 将光能引入微腔中，例如环形或球形微腔。 光能的一部分被反向散射并与引入的光能相互作用以在相应的第一和第二频率处形成第一和第二光能模式，也被称为分裂频率或模式双峰。 一个或多个分子结合到微腔的外表面并与在微腔内共振的光能的消逝场相互作用。 至少部分地基于分割频率相对于基线分割频率的变化来检测一个或多个分子与外表面的结合。
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44.发明授权Dynamic motion contrast and transverse flow estimation using optical coherence tomography 有权使用光学相干断层扫描的动态运动对比度和横向流量估计公开(公告)号：US08369594B2
公开(公告)日：2013-02-05
申请号：US13179377
申请日：2011-07-08
CPC分类号： A61B5/0261 , A61B3/102 , A61B5/0066 , G01N21/4795 , G01N2021/1787
摘要： The methods described herein are methods to ascertain motion contrast within optical coherence tomography data based upon phase variance. The phase variance contrast observes the nanometer scale motion of scatterers associated with Brownian motion and other non-flow motion. The inventive method of calculating motion contrast from the phase variance can differentiate regions of different mobility based on the motion contrast differences, and can use the phase information to characterize mobility properties of the scatterers. In flow regions, the inventive method for acquiring and analyzing motion contrast can identify the regions as well as characterize the motion. Furthermore, the inventive method can determine quantitative flow estimation, the index of refraction variations, and absorption variations within flow regions.
摘要翻译： 本文描述的方法是基于相位方差来确定光学相干断层摄影数据内的运动对比度的方法。 相位差对比度观察与布朗运动和其他非流动运动相关的散射体的纳米级运动。 从相位方差计算运动对比度的本发明方法可以基于运动对比差异来区分不同移动性的区域，并且可以使用相位信息来表征散射体的移动性质。 在流动区域中，用于获取和分析运动对比度的本发明的方法可以识别区域以及表征运动。 此外，本发明的方法可以确定流量区域中的定量流量估计，折射率变化指数和吸收变化。
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45.发明申请DYNAMIC MOTION CONTRAST AND TRANSVERSE FLOW ESTIMATION USING OPTICAL COHERENCE TOMOGRAPHY 有权动态运动对比度和横向流量估计使用光学相干计算公开(公告)号：US20120004562A1
公开(公告)日：2012-01-05
申请号：US13179377
申请日：2011-07-08
IPC分类号： A61B5/02
CPC分类号： A61B5/0261 , A61B3/102 , A61B5/0066 , G01N21/4795 , G01N2021/1787
摘要： The methods described herein are methods to ascertain motion contrast within optical coherence tomography data based upon phase variance. The phase variance contrast observes the nanometer scale motion of scatterers associated with Brownian motion and other non-flow motion. The inventive method of calculating motion contrast from the phase variance can differentiate regions of different mobility based on the motion contrast differences, and can use the phase information to characterize mobility properties of the scatterers. In flow regions, the inventive method for acquiring and analyzing motion contrast can identify the regions as well as characterize the motion. Furthermore, the inventive method can determine quantitative flow estimation, the index of refraction variations, and absorption variations within flow regions.
摘要翻译： 本文描述的方法是基于相位方差来确定光学相干断层摄影数据内的运动对比度的方法。 相位差对比度观察与布朗运动和其他非流动运动相关的散射体的纳米级运动。 从相位方差计算运动对比度的本发明方法可以基于运动对比差异来区分不同移动性的区域，并且可以使用相位信息来表征散射体的移动性质。 在流动区域中，用于获取和分析运动对比度的本发明的方法可以识别区域以及表征运动。 此外，本发明的方法可以确定流量区域中的定量流量估计，折射率变化指数和吸收变化。
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公开(公告)号：US20110104676A1
公开(公告)日：2011-05-05
申请号：US12790379
申请日：2010-05-28
申请人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
发明人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
IPC分类号： C12Q1/68 , G01N33/566 , G01N33/50
CPC分类号： C12Q1/682 , C12Q1/6818 , C12Q1/6841 , Y10T436/143333 , C12Q2563/107 , C12Q2537/149 , C12Q2525/301
摘要： The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression.
摘要翻译： 本发明涉及使用荧光标记的核酸探针来鉴定和成像生物样品中的分析物。 在优选的实施方案中，提供了包含能够特异性结合目标分析物的靶区域和能够引发核酸单体聚合的引发区域的探针。 在将样品与探针接触后，提供形成系留聚合物的标记单体。 触发的探针和自熄单体可用于提供活性背景抑制。
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公开(公告)号：US07407814B2
公开(公告)日：2008-08-05
申请号：US09927779
申请日：2001-08-09
IPC分类号： G01N33/543
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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公开(公告)号：US06403332B1
公开(公告)日：2002-06-11
申请号：US09628219
申请日：2000-07-28
IPC分类号： C12Q102
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6417 , G01N2021/6421 , Y10S435/968
摘要： A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.
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公开(公告)号：US08310677B2
公开(公告)日：2012-11-13
申请号：US13361076
申请日：2012-01-30
IPC分类号： G01N21/00
CPC分类号： G01N21/7746 , G01N21/783 , G01N33/0037 , G01N33/0047 , G01N33/0049 , G01N33/0054 , G01N33/22 , G01N2021/773 , G01N2021/7789 , Y02A50/245 , Y02A50/246 , Y02A50/25
摘要： Micro-cavity gas or vapor sensors and gas or vapor detection methods. Optical energy is introduced into a resonant micro-cavity having a deformable coating such as a polymer. The coating swells or expands when it is exposed to or absorbs a gas or vapor, thereby changing the resonant wavelength of optical energy circulating within the micro-cavity/coating. Expansion or swelling of the coating may be reversible such that it contracts when gas or vapor diffuses from the coating. The coating deformation and/or a change of one or more optical properties of the optical energy circulating within the micro-cavity are used to detect the presence of the gas or vapor or molecules or particulates thereof.
摘要翻译： 微腔气体或蒸气传感器以及气体或蒸气检测方法。 将光能引入具有可变形涂层如聚合物的共振微腔中。 当涂层暴露于或吸收气体或蒸气时，涂层膨胀或膨胀，从而改变在微腔/涂层内循环的光能的共振波长。 涂层的膨胀或膨胀可以是可逆的，使得当气体或蒸汽从涂层扩散时，其膨胀或膨胀。 使用在微腔内循环的光能的涂层变形和/或一个或多个光学特性的变化来检测气体或蒸气或其分子或微粒的存在。
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50.发明授权Dynamic motion contrast and transverse flow estimation using optical coherence tomography 有权使用光学相干断层扫描的动态运动对比度和横向流量估计公开(公告)号：US07995814B2
公开(公告)日：2011-08-09
申请号：US11767187
申请日：2007-06-22
CPC分类号： A61B5/0261 , A61B3/102 , A61B5/0066 , G01N21/4795 , G01N2021/1787
摘要： The methods described herein are methods to ascertain motion contrast within optical coherence tomography data based upon phase variance. The phase variance contrast observes the nanometer scale motion of scatterers associated with Brownian motion and other non-flow motion. The inventive method of calculating motion contrast from the phase variance can differentiate regions of different mobility based on the motion contrast differences, and can use the phase information to characterize mobility properties of the scatterers. In flow regions, the inventive method for acquiring and analyzing motion contrast can identify the regions as well as characterize the motion. Furthermore, the inventive method can determine quantitative flow estimation, the index of refraction variations, and absorption variations within flow regions.
摘要翻译： 本文描述的方法是基于相位方差来确定光学相干断层摄影数据内的运动对比度的方法。 相位差对比度观察与布朗运动和其他非流动运动相关的散射体的纳米级运动。 从相位方差计算运动对比度的本发明方法可以基于运动对比差异来区分不同移动性的区域，并且可以使用相位信息来表征散射体的移动性质。 在流动区域中，用于获取和分析运动对比度的本发明的方法可以识别区域以及表征运动。 此外，本发明的方法可以确定流量区域中的定量流量估计，折射率变化指数和吸收变化。
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