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公开(公告)号：US06750036B2
公开(公告)日：2004-06-15
申请号：US10159703
申请日：2002-05-28
IPC分类号： C12Q102
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6417 , G01N2021/6421 , Y10S435/968
摘要： A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.
摘要翻译： 用于监测细胞样本中的细胞活性的系统和方法。 根据一个实施例，将多个可激发标记施加到样本。 提供多光子激光显微镜来激发样品的区域并引起从该区域辐射的荧光。 辐射荧光由光谱分析仪处理，将荧光分离成相应的波段。 然后通过一组检测器收集各个荧光带，每个检测器接收相应的一个波长带。
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公开(公告)号：US20090095919A1
公开(公告)日：2009-04-16
申请号：US12335380
申请日：2008-12-15
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6417 , G01N2021/6421 , Y10S435/968
摘要： A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.
摘要翻译： 用于监测细胞样本中的细胞活性的系统和方法。 根据一个实施例，将多个可激发标记施加到样本。 提供多光子激光显微镜来激发样品的区域并引起从该区域辐射的荧光。 辐射荧光由光谱分析仪处理，将荧光分离成相应的波段。 然后通过一组检测器收集各个荧光带，每个检测器接收相应的一个波长带。
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公开(公告)号：US06403332B1
公开(公告)日：2002-06-11
申请号：US09628219
申请日：2000-07-28
IPC分类号： C12Q102
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6417 , G01N2021/6421 , Y10S435/968
摘要： A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.
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4.发明授权公开(公告)号：US09116128B2
公开(公告)日：2015-08-25
申请号：US13346484
申请日：2012-01-09
IPC分类号： G01N33/547 , G01N33/553 , G01N33/543 , G01N21/77
CPC分类号： G01N21/7746 , G01N33/54353 , G01N2021/7789
摘要： Micro-cavity resonant sensors have outer surfaces that are functionalized using click chemistry, e.g., involving a cycloaddition reaction of an alkyne functional group and an azide functional group. A first polymer linking element binds to an outer surface of the micro-cavity and has an azide functional group, which bonds to an alkyne functional group of a second polymer linking element as a result of a cycloaddition reaction. A functionalization element such as an antibody, antigen or protein for sensing a target molecule is bound to the second linking element.
摘要翻译： 微腔谐振传感器具有使用点击化学功能化的外表面，例如涉及炔官能团和叠氮官能团的环加成反应。 第一聚合物连接元件与微腔的外表面结合并具有叠氮化物官能团，其结合环加成反应的结果与第二聚合物连接元素的炔官能团结合。 用于感测靶分子的功能化元件如抗体，抗原或蛋白质被结合到第二连接元件。
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公开(公告)号：US08507204B2
公开(公告)日：2013-08-13
申请号：US13363022
申请日：2012-01-31
申请人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
发明人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6818 , C12Q1/6841 , Y10T436/143333 , C12Q2563/107 , C12Q2537/149 , C12Q2525/301
摘要： The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression.
摘要翻译： 本发明涉及使用荧光标记的核酸探针来鉴定和成像生物样品中的分析物。 在优选的实施方案中，提供了包含能够特异性结合目标分析物的靶区域和能够引发核酸单体聚合的引发区域的探针。 在将样品与探针接触后，提供形成系留聚合物的标记单体。 触发的探针和自熄单体可用于提供活性背景抑制。
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公开(公告)号：US08329452B2
公开(公告)日：2012-12-11
申请号：US13183336
申请日：2011-07-14
IPC分类号： C12M1/34
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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公开(公告)号：US20120120398A1
公开(公告)日：2012-05-17
申请号：US13361076
申请日：2012-01-30
IPC分类号： G01N21/00
CPC分类号： G01N21/7746 , G01N21/783 , G01N33/0037 , G01N33/0047 , G01N33/0049 , G01N33/0054 , G01N33/22 , G01N2021/773 , G01N2021/7789 , Y02A50/245 , Y02A50/246 , Y02A50/25
摘要： Micro-cavity gas or vapor sensors and gas or vapor detection methods. Optical energy is introduced into a resonant micro-cavity having a deformable coating such as a polymer. The coating swells or expands when it is exposed to or absorbs a gas or vapor, thereby changing the resonant wavelength of optical energy circulating within the micro-cavity/coating. Expansion or swelling of the coating may be reversible such that it contracts when gas or vapor diffuses from the coating. The coating deformation and/or a change of one or more optical properties of the optical energy circulating within the micro-cavity are used to detect the presence of the gas or vapor or molecules or particulates thereof
摘要翻译： 微腔气体或蒸气传感器以及气体或蒸气检测方法。 将光能引入具有可变形涂层如聚合物的共振微腔中。 当涂层暴露于或吸收气体或蒸气时，涂层膨胀或膨胀，从而改变在微腔/涂层内循环的光能的共振波长。 涂层的膨胀或膨胀可以是可逆的，使得当气体或蒸汽从涂层扩散时，其膨胀或膨胀。 使用在微腔内循环的光能的涂层变形和/或一种或多种光学特性的变化来检测气体或蒸汽或其分子或颗粒的存在
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8.发明申请公开(公告)号：US20120107177A1
公开(公告)日：2012-05-03
申请号：US13346484
申请日：2012-01-09
IPC分类号： G01N33/53
CPC分类号： G01N21/7746 , G01N33/54353 , G01N2021/7789
摘要： Micro-cavity resonant sensors have outer surfaces that are functionalized using click chemistry, e.g., involving a cycloaddition reaction of an alkyne functional group and an azide functional group. A first polymer linking element binds to an outer surface of the micro-cavity and has an azide functional group, which bonds to an alkyne functional group of a second polymer linking element as a result of a cycloaddition reaction. A functionalization element such as an antibody, antigen or protein for sensing a target molecule is bound to the second linking element.
摘要翻译： 微腔谐振传感器具有使用点击化学功能化的外表面，例如涉及炔官能团和叠氮官能团的环加成反应。 第一聚合物连接元件与微腔的外表面结合并具有叠氮化物官能团，其结合环加成反应的结果与第二聚合物连接元素的炔官能团结合。 用于感测靶分子的功能化元件如抗体，抗原或蛋白质被结合到第二连接元件。
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9.发明授权公开(公告)号：US08092855B2
公开(公告)日：2012-01-10
申请号：US12324605
申请日：2008-11-26
IPC分类号： G01N1/28
CPC分类号： G01N21/7746 , G01N33/54353 , G01N2021/7789
摘要： Micro-cavity resonant sensors have outer surfaces that are functionalized using click chemistry, e.g., involving a cycloaddition reaction of an alkyne functional group and an azide functional group. A first polymer linking element binds to an outer surface of the micro-cavity and has an azide functional group, which bonds to an alkyne functional group of a second polymer linking element as a result of a cycloaddition reaction. A functionalization element such as an antibody, antigen or protein for sensing a target molecule is bound to the second linking element.
摘要翻译： 微腔谐振传感器具有使用点击化学功能化的外表面，例如涉及炔官能团和叠氮官能团的环加成反应。 第一聚合物连接元件与微腔的外表面结合并具有叠氮化物官能团，其结合环加成反应的结果与第二聚合物连接元素的炔官能团结合。 用于感测靶分子的功能化元件如抗体，抗原或蛋白质被结合到第二连接元件。
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公开(公告)号：US20100233820A1
公开(公告)日：2010-09-16
申请号：US12690863
申请日：2010-01-20
CPC分类号： G01N21/6458 , B82Y15/00 , B82Y20/00 , C12Q1/6818 , G01N21/636 , G01N21/6428 , G01N2021/6441 , G02F1/37 , G02F2202/36 , Y10T436/143333 , C12Q2563/155 , C12Q2535/101
摘要： Second harmonic nanoprobes for multipurpose imaging of samples and a method of using such probes to monitor nucleotide sequencing in a Multi-SHG Detection Imaging (MSDI) modality and to monitor external electric field using voltage sensitive second harmonic generating (SHG) nanoprobes are provided. The SHG nanoprobes are comprised of various kinds of nanocrystals that do not possess an inversion symmetry and therefore are capable of generating second harmonic signals that can then be detected by conventional two-photon microscopy for in vivo imaging of biological processes and structures such as cell signaling, neuroimaging, protein conformation probing, DNA conformation probing, gene transcription, virus infection and replication in cells, protein dynamics, tumor imaging and cancer therapy evaluation and diagnosis as well as quantification in optical imaging for a wide-range of biological and non-biological processes and devices.
摘要翻译： 提供了用于样本多功能成像的二次谐波纳米探针和使用这种探针监测多SHG检测成像（MSDI）模式中的核苷酸测序并使用电压敏感二次谐波产生（SHG）纳米探针监测外部电场的方法。 SHG纳米探针由不具有反转对称性的各种纳米晶体组成，因此能够产生二次谐波信号，然后可以通过常规双光子显微镜检测生物过程和结构如体细胞信号传导的体内成像 ，神经成像，蛋白质构象探索，DNA构象探测，基因转录，细胞中的病毒感染和复制，蛋白质动力学，肿瘤成像和癌症治疗评估和诊断，以及广泛的生物和非生物学的光学成像定量 流程和设备。
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