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    SYSTEM AND METHOD FOR MONITORING CELLULAR ACTIVITY
    1.
    发明申请
    SYSTEM AND METHOD FOR MONITORING CELLULAR ACTIVITY 审中-公开
    用于监测细胞活性的系统和方法

    公开(公告)号：US20090095919A1

    公开(公告)日：2009-04-16

    申请号：US12335380

    申请日：2008-12-15

    申请人： Gregory H. Bearman ,  Scott E. Fraser ,  Russell D. Lansford

    发明人： Gregory H. Bearman ,  Scott E. Fraser ,  Russell D. Lansford

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6417 ,  G01N2021/6421 ,  Y10S435/968

    摘要： A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.

    摘要翻译： 用于监测细胞样本中的细胞活性的系统和方法。 根据一个实施例，将多个可激发标记施加到样本。 提供多光子激光显微镜来激发样品的区域并引起从该区域辐射的荧光。 辐射荧光由光谱分析仪处理，将荧光分离成相应的波段。 然后通过一组检测器收集各个荧光带，每个检测器接收相应的一个波长带。

    System and method for monitoring cellular activity
    2.
    发明授权
    System and method for monitoring cellular activity 有权
    用于监测细胞活性的系统和方法

    公开(公告)号：US06750036B2

    公开(公告)日：2004-06-15

    申请号：US10159703

    申请日：2002-05-28

    申请人： Gregory H. Bearman ,  Scott E. Fraser ,  Russell D. Lansford

    发明人： Gregory H. Bearman ,  Scott E. Fraser ,  Russell D. Lansford

    IPC分类号： C12Q102

    CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6417 ,  G01N2021/6421 ,  Y10S435/968

    摘要： A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.

    摘要翻译： 用于监测细胞样本中的细胞活性的系统和方法。 根据一个实施例，将多个可激发标记施加到样本。 提供多光子激光显微镜来激发样品的区域并引起从该区域辐射的荧光。 辐射荧光由光谱分析仪处理，将荧光分离成相应的波段。 然后通过一组检测器收集各个荧光带，每个检测器接收相应的一个波长带。

    SINGLE-LENS COMPUTED TOMOGRAPHY IMAGING SPECTROMETER AND METHOD OF CAPTURING SPATIAL AND SPECTRAL INFORMATION
    6.
    发明申请
    SINGLE-LENS COMPUTED TOMOGRAPHY IMAGING SPECTROMETER AND METHOD OF CAPTURING SPATIAL AND SPECTRAL INFORMATION 有权
    单镜计算机图像成像光谱仪和捕获空间和光谱信息的方法

    公开(公告)号：US20090180115A1

    公开(公告)日：2009-07-16

    申请号：US12352576

    申请日：2009-01-12

    申请人： Daniel W. Wilson ,  William R. Johnson ,  Gregory H. Bearman

    发明人： Daniel W. Wilson ,  William R. Johnson ,  Gregory H. Bearman

    IPC分类号： G01J3/28

    CPC分类号： G01J3/02 ,  G01J3/0208 ,  G01J3/1838 ,  G01J3/2823 ,  G01N21/31 ,  G01N2021/317

    摘要： Computed tomography imaging spectrometers (“CTISs”) employing a single lens are provided. The CTISs may be either transmissive or reflective, and the single lens is either configured to transmit and receive uncollimated light (in transmissive systems), or is configured to reflect and receive uncollimated light (in reflective systems). An exemplary transmissive CTIS includes a focal plane array detector, a single lens configured to transmit and receive uncollimated light, a two-dimensional grating, and a field stop aperture. An exemplary reflective CTIS includes a focal plane array detector, a single mirror configured to reflect and receive uncollimated light, a two-dimensional grating, and a field stop aperture.

    摘要翻译： 提供了使用单个透镜的计算机断层扫描成像光谱仪（“CTIS”）。 CTIS可以是透射的或反射的，并且单个透镜被配置为发射和接收未预光的光（在透射系统中），或被配置为反射和接收未预激光（在反射系统中）。 示例性透射CTIS包括焦平面阵列检测器，被配置为发射和接收未预激光的单个透镜，二维光栅和场停止孔。 示例性反射CTIS包括焦平面阵列检测器，配置成反射和接收未预激光的单反射镜，二维光栅和场光圈。

    Methods and apparatus and assays of bacterial spores
    7.
    发明授权
    Methods and apparatus and assays of bacterial spores 有权
    细菌孢子的方法和装置和测定

    公开(公告)号：US08173359B2

    公开(公告)日：2012-05-08

    申请号：US11810005

    申请日：2007-06-04

    申请人： Adrian Ponce ,  Gregory H. Bearman

    发明人： Adrian Ponce ,  Gregory H. Bearman

    IPC分类号： C12Q1/00 ,  C12Q1/06 ,  G01N33/20

    CPC分类号： C12Q1/04 ,  G01N21/6408 ,  G01N33/54306 ,  G01N33/56911 ,  G01N33/84

    摘要： A sample of unknown bacterial spores is added to a test strip. The sample of unknown bacterial spores is drawn to a first sample region on the test strip by capillary action. Species-specific antibodies are bound to the sample when the unknown bacterial spores match the species-specific antibodies, otherwise the sample is left unbound. DPA is released from the bacterial spores in the bound sample. The terbium ions are combined with the DPA to form a Tb-DPA complex. The combined terbium ions and DPA are excited to generate a luminescence characteristic of the combined terbium ions and DPA to detect the bacterial spores. A live/dead assay is performed by a release of the DPA for live spores and a release of DPA for all spores. The detection concentrations are compared to determine the fraction of live spores. Lifetime-gated measurements of bacterial spores to eliminate any fluorescence background from organic chromophores comprise labeling the bacterial spore contents with a long-lifetime lumophore and detecting the luminescence after a waiting period. Unattended monitoring of bacterial spores in the air comprises the steps of collecting bacterial spores carried in the air and repeatedly performing the Tb-DPA detection steps above. The invention is also apparatus for performing the various methods disclosed above.

    摘要翻译： 将未知细菌孢子的样品加入到测试条中。 未知细菌孢子的样品通过毛细管作用被吸引到测试条上的第一样品区域。 当未知细菌孢子与物种特异性抗体匹配时，特异性抗体与样品结合，否则样品保持未结合。 DPA从结合样品中的细菌孢子中释放出来。 铽离子与DPA组合形成Tb-DPA复合物。 激发组合的铽离子和DPA以产生组合的铽离子和DPA的发光特性以检测细菌孢子。 通过释放活孢子的DPA和释放所有孢子的DPA来进行活/死测定。 比较检测浓度以确定活孢子的分数。 细菌孢子的终生门控测量以消除有机发色团中的任何荧光背景包括用长寿命的荧光团标记细菌孢子含量并在等待期后检测发光。 空气中细菌孢子的无人值守监测包括收集空气中携带的细菌孢子并重复进行上述Tb-DPA检测步骤的步骤。 本发明也是用于执行上述各种方法的装置。

    Single-lens computed tomography imaging spectrometer and method of capturing spatial and spectral information
    8.
    发明授权
    Single-lens computed tomography imaging spectrometer and method of capturing spatial and spectral information 有权
    单透镜计算机断层成像光谱仪和捕获空间和光谱信息的方法

    公开(公告)号：US07894058B2

    公开(公告)日：2011-02-22

    申请号：US12352576

    申请日：2009-01-12

    申请人： Daniel W. Wilson ,  William R. Johnson ,  Gregory H. Bearman

    发明人： Daniel W. Wilson ,  William R. Johnson ,  Gregory H. Bearman

    IPC分类号： G01J3/28

    CPC分类号： G01J3/02 ,  G01J3/0208 ,  G01J3/1838 ,  G01J3/2823 ,  G01N21/31 ,  G01N2021/317

    摘要： Computed tomography imaging spectrometers (“CTISs”) employing a single lens are provided. The CTISs may be either transmissive or reflective, and the single lens is either configured to transmit and receive uncollimated light (in transmissive systems), or is configured to reflect and receive uncollimated light (in reflective systems). An exemplary transmissive CTIS includes a focal plane array detector, a single lens configured to transmit and receive uncollimated light, a two-dimensional grating, and a field stop aperture. An exemplary reflective CTIS includes a focal plane array detector, a single mirror configured to reflect and receive uncollimated light, a two-dimensional grating, and a field stop aperture.

    摘要翻译： 提供了使用单个透镜的计算机断层扫描成像光谱仪（“CTIS”）。 CTIS可以是透射的或反射的，并且单个透镜被配置为发射和接收未预光的光（在透射系统中），或被配置为反射和接收未预激光（在反射系统中）。 示例性透射CTIS包括焦平面阵列检测器，被配置为发射和接收未预激光的单个透镜，二维光栅和场停止孔。 示例性反射CTIS包括焦平面阵列检测器，配置成反射和接收未预激光的单反射镜，二维光栅和场光圈。

    Method of detecting vulnerable atherosclerotic plaque
    10.
    发明授权
    Method of detecting vulnerable atherosclerotic plaque 有权
    检测易受损的动脉粥样硬化斑块的方法

    公开(公告)号：US06475159B1

    公开(公告)日：2002-11-05

    申请号：US09268609

    申请日：1999-03-15

    申请人： S. Ward Casscells ,  James T. Willerson ,  Gregory H. Bearman ,  Michael L. Eastwood ,  Timothy N. Krabach

    发明人： S. Ward Casscells ,  James T. Willerson ,  Gregory H. Bearman ,  Michael L. Eastwood ,  Timothy N. Krabach

    IPC分类号： A61B500

    CPC分类号： A61B5/0086 ,  A61B5/0066 ,  A61B5/0071 ,  A61B5/0073 ,  A61B5/0075 ,  A61B5/01 ,  A61B5/015 ,  A61B5/02007 ,  A61B5/0507 ,  A61B5/413 ,  A61B5/6853 ,  A61B2017/00084 ,  A61B2017/22001

    摘要： An infrared, heat-sensing catheter particularly useful for identifying potentially fatal arterial plaques in patients with disease of the coronary or other arteries and its use are detailed. In one embodiment, an infrared fiberoptic system (with or without ultrasound) is employed at the tip of the catheter to locate inflamed, heat-producing, atherosclerotic plaque, which is at greater risk for rupture, fissure, or ulceration, and consequent thrombosis and occlusion of the artery. In another embodiment, a catheter with an infrared detector (with or without ultrasound) employed at its tip will likewise locate inflamed heat-producing atherosclerotic plaque. The devices and methods of the invention may be used to detect abscesses, infection, and cancerous regions by the heat such regions differentially display over the ambient temperature of immediately adjacent tissues. The methods and devices of the invention may also be used to detect regions of cooler than ambient tissue in a vessel or organ which indicate cell death, thrombosis, cell death, hemorrhage, calcium or cholesterol accumulations, or foreign materials.

    摘要翻译： 详细描述了特别用于识别患有冠状动脉或其他动脉疾病的患者中的潜在致命动脉斑块的红外线，热感应导管。 在一个实施例中，在导管的尖端处使用红外光纤系统（具有或不具有超声波）以定位发炎的，发热的动脉粥样硬化斑块，其具有破裂，裂隙或溃疡的更大风险以及由此导致的血栓形成和 动脉闭塞。 在另一个实施例中，具有在其尖端处使用的具有或不具有超声波的红外检测器（具有或不具有超声波）的导管将同样定位发炎的发热动脉粥样硬化斑块。 本发明的装置和方法可以用于通过这些区域在紧邻的组织的环境温度上差异显示的热量来检测脓肿，感染和癌性区域。 本发明的方法和装置还可用于检测指示细胞死亡，血栓形成，细胞死亡，出血，钙或胆固醇积聚或外来物质的血管或器官中比周围组织更冷的区域。