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    SINGLE MOLECULE ELECTRONIC MULTIPLEX SNP ASSAY AND PCR ANALYSIS
    41.
    发明申请
    SINGLE MOLECULE ELECTRONIC MULTIPLEX SNP ASSAY AND PCR ANALYSIS 审中-公开
    单分子电子多重SNP测定和PCR分析

    公开(公告)号：US20170058335A1

    公开(公告)日：2017-03-02

    申请号：US15118812

    申请日：2015-02-12

    申请人： Chuanjuan Tao ,  Shiv Kumar ,  Minchen Chien ,  Jingyue Ju

    发明人： Chuanjuan Tao ,  Shiv Kumar ,  Minchen Chien ,  Jingyue Ju

    IPC分类号： C12Q1/68

    摘要： This invention provides methods of using labeled primers or probes for nucleic acid target detection and to detect the identity or presence of a nucleotide at certain positions in nucleic acid sequences with single molecule sensitivity using nanopore detection, and sets of oligonucleotide primers for use in such methods, as well as methods of quantitative PCR coupled with nanopore detection.

    摘要翻译： 本发明提供使用标记的引物或探针用于核酸靶标检测的方法，并使用纳米孔检测法检测具有单分子敏感性的核酸序列的某些位置的核苷酸的同一性或存在，以及用于这些方法的寡核苷酸引物组 ，以及与纳米孔检测结合的定量PCR方法。

    Primers labeled with energy transfer coupled dyes for DNA sequencing
    45.
    发明授权
    Primers labeled with energy transfer coupled dyes for DNA sequencing 失效
    用能量转移偶联染料标记用于DNA测序的引物

    公开(公告)号：US5707804A

    公开(公告)日：1998-01-13

    申请号：US410808

    申请日：1995-03-27

    申请人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    发明人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    IPC分类号： G01N33/53 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/542 ,  G01N33/566 ,  G01N33/58 ,  C07H21/04

    CPC分类号： G01N33/582 ,  G01N33/542 ,  Y10S435/81

    摘要： Compositions are provided for making oligonucleotides carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donor at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair, and to have enhanced emission intensities from the acceptor. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

    摘要翻译： 提供组合物用于制备携带供体和受体染料分子对的寡核苷酸，设计用于在单个波长下有效激发供体，并以不同波长的每对中的受体发射。 具有不同供体 - 受体对的不同分子可以通过改变给定对中的供体和受体之间的距离并且具有来自受体的增强的发射强度而在分离条件下具有基本上相同的迁移率。 特别地，荧光组合物在测序核酸中可用作标记。

    NUCLEIC ACID SEQUENCES USING TAGS
    46.
    发明申请
    NUCLEIC ACID SEQUENCES USING TAGS 审中-公开

    公开(公告)号：US20200248250A1

    公开(公告)日：2020-08-06

    申请号：US16735067

    申请日：2020-01-06

    申请人： Sergey Kalachikov ,  Jingyue Ju ,  Irina Morozova ,  Michael Dorwart

    发明人： Sergey Kalachikov ,  Jingyue Ju ,  Irina Morozova ,  Michael Dorwart

    IPC分类号： C12Q1/6869 ,  G01N33/487 ,  G01N27/447

    摘要： This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides arc provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.