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104 条记录 (耗时 0.029 秒)

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    41.
    发明授权
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US07309574B2

    公开(公告)日：2007-12-18

    申请号：US11203417

    申请日：2005-08-15

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6858 ,  C12Q2535/131 ,  C12Q2535/125

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of A, T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当靶SNP碱基的3'侧邻近的碱基为A时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 一个基座间隔开的是G.根据本发明，3'端基被设计为对应于SNP的基极; 从3'的第二个底部到C; 从3'端的第三个基地是A，T或G中的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    45.
    发明申请
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权

    公开(公告)号：US20070042393A1

    公开(公告)日：2007-02-22

    申请号：US11333352

    申请日：2006-01-18

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6813

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is T. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of T, C or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    Signal detecting sensor provided with multi-electrode
    47.
    发明授权
    Signal detecting sensor provided with multi-electrode 有权
    信号检测传感器配有多电极

    公开(公告)号：US07006929B2

    公开(公告)日：2006-02-28

    申请号：US10479938

    申请日：2002-06-05

    申请人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara ,  Satoshi Konishi

    发明人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara ,  Satoshi Konishi

    IPC分类号： G06F19/00

    CPC分类号： G01N33/48728

    摘要： A sensor capable of simply and quickly measuring the electrical activities of a number of cells simultaneously and a device comprising the sensor and capable of detecting the signal transmission between subject cells are provided. The sensor comprises a plurality of electrodes provided on a substrate. The electrodes individually measure a signal generated by a biological sample held by each of the electrodes. Each of the electrodes comprises a depression for holding the biological sample, a throughhole which is in communication with the depression and penetrates to a rear side of the substrate, an electrode portion, and a drawing line from the electrode portion. The electrodes are arranged so that the samples held by the depressions electrically communicate with one another.

    摘要翻译： 提供了能够简单且快速地同时测量多个单元的电活动的传感器，以及包括该传感器并且能够检测主体单元之间的信号传输的设备。 传感器包括设置在基板上的多个电极。 电极单独地测量由每个电极保持的生物样品产生的信号。 每个电极包括用于保持生物样品的凹陷，与凹陷连通并穿透到基底的后侧的通孔，电极部分和来自电极部分的引出线。 电极被布置成使得由凹陷保持的样品彼此电连通。

    Method for manufacturing solar cell using silicon powder
    49.
    发明授权
    Method for manufacturing solar cell using silicon powder 失效
    使用硅粉制造太阳能电池的方法

    公开(公告)号：US08603850B2

    公开(公告)日：2013-12-10

    申请号：US13591628

    申请日：2012-08-22

    申请人： Hiroaki Oka ,  Nariaki Oka

    发明人： Hiroaki Oka ,  Nariaki Oka

    IPC分类号： H01L21/00 ,  H01L21/324 ,  H01L21/477

    CPC分类号： H01L31/1804 ,  C23C24/10 ,  H01L31/068 ,  Y02E10/547 ,  Y02P70/521

    摘要： To provide a method for manufacturing a solar cell, whereby solar cells can be mass-produced by a simple process at low cost. A first conductivity-type silicon powder (11) is prepared, a silicon powder layer (11a) is formed by disposing the powder in the form of a layer, the powder layer is melted by heating the powder layer to the melting point of silicon or higher, and a first conductivity-type silicon layer (11b) is formed by cooling the melted layer. A second conductivity-type silicon powder (12) is prepared, a second conductivity-type silicon powder layer (12a) is formed by disposing the powder in the form of a layer on the first conductivity-type silicon layer (11b), the powder layer is melted by heating the powder layer to the melting point of silicon or higher, and a second conductivity-type silicon layer (12b) is formed by cooling the melted layer.

    摘要翻译： 为了提供太阳能电池的制造方法，能够以简单的方法以低成本大量生产太阳能电池。 制备第一导电型硅粉末（11），通过将粉末以层的形式设置形成硅粉末层（11a），通过将粉末层加热至硅的熔点或熔融温度 并且通过冷却熔融层形成第一导电型硅层（11b）。 制备第二导电型硅粉末（12），通过在第一导电型硅层（11b）上设置层状的粉末形成第二导电型硅粉末层（12a），粉末 通过将粉末层加热到硅的熔点以上来熔融层，并且通过冷却熔融层形成第二导电型硅层（12b）。

    METHOD FOR PRODUCING cAMP USING CHIMERIC OLFACTORY RECEPTOR
    50.
    发明申请
    METHOD FOR PRODUCING cAMP USING CHIMERIC OLFACTORY RECEPTOR 有权
    生产使用二甲基胆碱受体的cAMP的方法

    公开(公告)号：US20130065244A1

    公开(公告)日：2013-03-14

    申请号：US13598294

    申请日：2012-08-29

    申请人： Masato SUZUKI ,  Hiroaki Oka ,  Shigeki Kiyonka ,  Yasuo Mori

    发明人： Masato SUZUKI ,  Hiroaki Oka ,  Shigeki Kiyonka ,  Yasuo Mori

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C07K14/705 ,  C07K14/47 ,  C07K14/70571 ,  C07K14/723 ,  C07K2319/00 ,  C07K2319/41 ,  C12P19/32 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/04

    摘要： A method for producing cAMP using a chimeric olfactory receptor. The method includes a step of preparing a reaction system comprising a first layer, a lipid bilayer membrane, and a second layer, and a step of supplying a chemical substance which stimulates the chimeric olfactory receptor to the first layer so as to produce the cAMP from ATP. The lipid bilayer membrane includes the chimeric olfactory receptor and adenylate cyclase. The chimeric olfactory receptor penetrates the lipid bilayer membrane. The second layer contains ATP and a G protein. The G protein is placed in the vicinity of one end of the chimeric olfactory receptor. The chimeric olfactory receptor is derived from a mouse olfactory receptor and the N-terminal of the chimeric olfactory receptor is modified with an amino acid sequence.

    摘要翻译： 使用嵌合嗅觉受体制备cAMP的方法。 该方法包括制备包括第一层，脂质双层膜和第二层的反应体系的步骤，以及将刺激嵌合嗅觉受体的化学物质提供给第一层以产生cAMP的步骤 ATP。 脂质双层膜包括嵌合嗅觉受体和腺苷酸环化酶。 嵌合嗅觉受体渗透脂质双层膜。 第二层含有ATP和G蛋白。 将G蛋白置于嵌合嗅觉受体的一端附近。 嵌合嗅觉受体来自小鼠嗅觉受体，嵌合嗅觉受体的N-末端用氨基酸序列修饰。