公开(公告)号：US20220127592A1

公开(公告)日：2022-04-28

申请号：US17571594

申请日：2022-01-10

申请人： Novozymes A/S

发明人： Hanne Vang Hendriksen ,  Gitte Budolfsen Lynglev ,  Henrik Frisner ,  Ciu Liu ,  Ye Liu ,  Eduardo Antonio Della Pia ,  Hans Peter Heldt-Hansen ,  Kenneth Jensen ,  Wei Peng ,  Ming Li

IPC分类号： C12N9/48 ,  C12P21/06

摘要： The present invention relates to a method for producing a protein hydrolysate using a polypeptide having endopeptidase activity and a polypeptide having carboxypeptidase activity and the use of these enzymes for hydrolysing a protein substrate. In addition, the present invention relates to polypeptides having carboxypeptidase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20220117247A1

公开(公告)日：2022-04-21

申请号：US17429555

申请日：2020-02-13

申请人： DUPONT NUTRITION BIOSCIENCES APS

发明人： Christophe FREMAUX ,  Philippe HORVATH ,  Armelle COCHU-BLACHÈRE ,  Dennis ROMERO ,  Sylvain MOINEAU ,  Simon LABRIE

IPC分类号： A23C9/123 ,  A23K10/18 ,  C12N9/48

摘要： The present invention relates to a lactic acid bacterium, in particular a strain of the Streptococcus genus, with reduced sensitivity to cos-type bacteriophages. The invention also relates to methods to engineer these lactic acid bacteria as well as their use to ferment milk.

公开(公告)号：US11299729B2

公开(公告)日：2022-04-12

申请号：US15567312

申请日：2016-04-15

申请人： President and Fellows of Harvard College

发明人： Ahmed Hussein Badran ,  David R. Liu

IPC分类号： C12N15/70 ,  C12N9/48 ,  C12N15/10 ,  C12N9/12

摘要： Strategies, reagents, methods, and systems for modulating the mutation rate in cells are provided herein. The strategies, reagents, methods, and systems are broadly applicable for the modulation of mutation rates in cells where high mutation rates and/or control over a broad range of mutation rates is desired, for example, in the context of diversifying a nucleic acid sequence or a plurality of such sequences within a population of cells, for the generation of diversified nucleic acid libraries, and for directed evolution of nucleic acids and encoded products.

公开(公告)号：US20220090039A1

公开(公告)日：2022-03-24

申请号：US17460141

申请日：2021-08-27

申请人： Codexis, Inc. ,  Societe des Produits Nestle S.A.

发明人： Chinping Chng ,  Nikki Dellas ,  Ravi David Garcia ,  Moulay Hicham Alaoui Ismaili ,  Kristen Jean Vallieu ,  Kerryn McCluskie

IPC分类号： C12N9/48 ,  A61K38/48 ,  A61P1/18

摘要： The present invention provides engineered protease polypeptides and compositions thereof. The engineered protease polypeptides have been optimized to provide improved activity, improved thermostability, protease stability, autolytic stability, and stability under a range of pH conditions, including acidic (pH 7) conditions. The invention also relates to the use of the compositions comprising the engineered protease polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered protease polypeptides, as well as methods for making the engineered polynucleotides and protease polypeptides.

公开(公告)号：US11274138B2

公开(公告)日：2022-03-15

申请号：US15940962

申请日：2018-03-29

申请人： MONSANTO TECHNOLOGY LLC

发明人： Edward Kraft ,  Renata Bolognesi ,  Artem Evdokimov ,  Farhad Moshiri ,  Meiying Zheng ,  Victor M. Guzov

IPC分类号： C07K14/705 ,  G01N33/50 ,  C12N9/16 ,  C07K14/435 ,  C12N9/48 ,  C12N9/64 ,  C12N15/63 ,  C12N5/10

摘要： The invention relates to identification and characterization of recombinant DNA and polypeptides for specific Bt toxin receptors. In particular, the Bt toxin receptors of the invention include those derived from the Lepidopteran super family including the species Trichoplusiani ni, Pseudoplusia includens, Helicoverpa zea, and Spodoptera frugiperda. The receptors of the invention further include those derived from the Coleopteran super family and particularly from the species Diabrotica virgifera virgifera. The recombinant DNA and polypeptides so provided are useful in the identification and design of novel Bt toxin receptor ligands including novel or improved insecticidal toxins for use in a variety of agricultural applications. Materials and methods for identifying novel toxins are also disclosed herein. The invention also provides methods for selecting toxins to combine to control insect populations by manipulating Bt toxin receptor.

公开(公告)号：US11248045B2

公开(公告)日：2022-02-15

申请号：US15739200

申请日：2016-06-24

申请人： JCR Pharmaceuticals Co., Ltd.

发明人： Hiroyuki Sonoda ,  Kenichi Takahashi

IPC分类号： C07K16/28 ,  C12N9/16 ,  A61K39/395 ,  C12N9/10 ,  C07K19/00 ,  C07K14/475 ,  C12N5/10 ,  A61K45/00 ,  C07K16/46 ,  C07K16/18 ,  C12N15/09 ,  C12N9/64 ,  C12N9/24 ,  C12N15/113 ,  A61P25/00 ,  C07K14/505 ,  C07K14/715 ,  C12N9/14 ,  C12N9/48 ,  A61K39/00

摘要： Disclosed are a means to convert compounds having physiological or pharmacological activity and unable to pass through the blood-brain barrier into a form that allows them to pass through the blood-brain barrier, and compounds converted thereby. The means is an anti-human transferrin receptor antibody and the converted compounds are molecular conjugates between physiologically active protein or pharmacologically active low-molecular-weight compounds and an anti-human transferrin receptor antibody.

公开(公告)号：US11241487B2

公开(公告)日：2022-02-08

申请号：US17001667

申请日：2020-08-24

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA ,  UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

发明人： Henry Daniell ,  Qiuhong Li ,  Mohan K. Raizada

IPC分类号： A61K38/48 ,  A61K38/16 ,  A61K36/23 ,  A61K36/28 ,  A61K36/31 ,  A61K47/65 ,  A61K47/64 ,  A61K9/00 ,  A61K9/16 ,  A61K9/19 ,  A61K9/50 ,  A61K38/22 ,  C12N15/82 ,  C07K14/28 ,  C12N9/48

摘要： Emerging evidence indicates that diminished activity of the vasoprotective axis of the renin-angiotensin system, constituting angiotensin converting enzyme2 (ACE2) and its enzymatic product, angiotensin-(1-7) [Ang-(1-7)] contribute to pulmonary hypertension (PH). However, clinical success for long-term delivery of ACE2 or Ang-(1-7) would require stability and ease of administration to increase patient compliance. Chloroplast expression of therapeutic proteins enables their bioencapsulation within plant cells to protect from acids and gastric enzymes; fusion to a transmucosal carrier facilitates effective systemic absorption. Oral feeding of rats with bioencapsulated ACE2 or Ang-(1-7) attenuated monocrotaline (MCT)-induced increase in right ventricular systolic pressure, decreased pulmonary vessel wall thickness and improved right heart function in both prevention and reversal protocols. Furthermore, combination of ACE2 and Ang-(1-7) augmented the beneficial effects against cardio-pulmonary pathophysiology induced by MCT administration.
Experiments have also been performed which indicate that this approach is also suitable for the treatment or inhibition of experimental uveitis and autoimmune uveoretinitis These studies provide proof-of-concept for a novel low-cost oral ACE2 or Ang-(1-7) delivery system using transplastomic technology for pulmonary and ocular disease therapeutics.

公开(公告)号：US11229687B2

公开(公告)日：2022-01-25

申请号：US16941454

申请日：2020-07-28

申请人： BioMarin Pharmaceutical Inc.

发明人： Thomas Lester ,  Saeed Moshashaee ,  Augustus O. Okhamafe ,  Charles A. O'Neill

IPC分类号： A61K31/198 ,  A61P25/28 ,  A61K38/48 ,  A61K9/00 ,  A61K47/02 ,  C12N9/48 ,  A61M5/14 ,  A61K9/08

摘要： Formulations comprising recombinant human tripeptidyl peptidase-1 (rhTPP1) for intrathecal, intracerebroventricular, or intraocular administration, and kits comprising the same, are disclosed. Methods of using rhTPP1 in the prevention and treatment of symptoms of Neuronal Ceroid Lipofuscinosis (CLN2) disease are also disclosed. The formulations and methods are effective in halting the progression of CLN2 disease and may be used to treat subjects having CLN2 or a family history of CLN2.

公开(公告)号：US20210380667A1

公开(公告)日：2021-12-09

申请号：US17284137

申请日：2019-10-08

申请人： Novozymes A/S

发明人： Poul Erik Pedersen ,  Esben Peter Friis ,  Carsten Andersen ,  Peter Rahbek Oestergaard ,  Johan Qvist

IPC分类号： C07K14/81 ,  C12P21/02 ,  C12N9/99 ,  C12N9/48

摘要： Disclosed are protease inhibitors capable of inhibiting the protease activity of a S1 or a S8 protease at ambient pH, but where the inhibitor further has a pH dependent binding to the S1 or the S8 inhibitor meaning that a complex of a S1 and a S8 protease and the inhibitor dissociates when pH is lowered to a pH below 6.0, e.g. to a pH value in the range of 4.0 to 6.0; e.g. in the range of 4.5 to 6.0; e.g. in the range of 4.5 to 5.5; whereby the protease activity is released.

公开(公告)号：US11134681B2

公开(公告)日：2021-10-05

申请号：US16785870

申请日：2020-02-10

申请人： Spogen Biotech Inc.

发明人： Brian Thompson ,  Katie Thompson

IPC分类号： C12N9/20 ,  C07K14/32 ,  A01N37/46 ,  A01N63/50 ,  A01N63/22 ,  A01N63/10 ,  A01N63/00 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  A01N37/48 ,  C12N9/42 ,  C12N9/48

摘要： The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.