利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

2950 条记录 (耗时 0.035 秒)

    High-throughput sequencing with semiconductor-based detection
    44.
    发明授权
    High-throughput sequencing with semiconductor-based detection 有权

    公开(公告)号：US11378544B2

    公开(公告)日：2022-07-05

    申请号：US16241902

    申请日：2019-01-07

    申请人： Illumina, Inc.

    发明人： Dietrich Dehlinger ,  Ali Agah ,  Tracy Helen Fung ,  Emrah Kostem

    IPC分类号： G01N27/414 ,  B01L3/00 ,  G06K9/62 ,  G01N21/64 ,  C12Q1/6874 ,  G06V10/75 ,  G01N21/76 ,  B01L7/00 ,  G16B30/00

    摘要： A biosensor for base calling is provided. The biosensor comprises a sampling device, which includes a sample surface that has an array of pixel areas and a solid-state imager that has an array of sensors. Each sensor generates pixel signals in each base calling cycle. Each pixel signal represents light gathered in one base calling cycle from one or more clusters in a corresponding pixel area of the sample surface. The biosensor further comprises a signal processor configured for connection to the sampling device. The signal processor receives and processes the pixel signals from the sensors for base calling in a base calling cycle, and uses the pixel signals from fewer sensors than a number of clusters base called in the base calling cycle. The pixel signals from the fewer sensors include at least one pixel signal representing light gathered from at least two clusters in the corresponding pixel area.

    LIGHT SOURCE ACCOMMODATION FOR DIFFERENT SAMPLE MATRICES
    45.
    发明申请
    LIGHT SOURCE ACCOMMODATION FOR DIFFERENT SAMPLE MATRICES 有权

    公开(公告)号：US20220155230A1

    公开(公告)日：2022-05-19

    申请号：US17545873

    申请日：2021-12-08

    申请人： PROCISEDX INC.

    发明人： MICHAEL HALE ,  STEFAN WESTIN ,  RAJ SRIKRISHNAN ,  RUKMINI REDDY

    IPC分类号： G01N21/64 ,  G01N21/76 ,  G01N33/72

    摘要： Provided herein are methods for accommodating for sample matrix effects in light measurement assays. A selected amount of a light emitting material is added to a sample, and the light output from the sample is measured. By using an algorithm based on the known light emitting material amount and the measured light output, a correction factor for the assay of the sample is determined. The correction factor can be used to adjust subsequent light output measurements of other samples recorded using the assay.

    Non-covalent complex-based sensors
    47.
    发明授权
    Non-covalent complex-based sensors 有权

    公开(公告)号：US11327023B2

    公开(公告)日：2022-05-10

    申请号：US16831545

    申请日：2020-03-26

    申请人： Texas Tech University System

    发明人： Gregory Fernandes

    IPC分类号： G01N21/76 ,  G01N21/64 ,  G01N33/58

    摘要： A system, method, and apparatus for sensing analytes comprises assembling a sensor of at least one polymer and at least one fluorophore by mixing the polymer with the fluorophore, exposing the sensor to an analyte, and identifying the presence of the analyte according to a change in fluorescence of the sensor. The sensor can comprise a non-covalent complex of the at least one fluorophore and the at least one polymer. The at least one polymer can comprise a polymer capable of binding a metal ion. The at least one polymer is capable of binding a metal ion comprising an imidazole-containing polymer such as an N-vinylimidazole homo polymer and/or an N-vinylimidazole co-polymer.

    USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS
    48.
    发明申请
    USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS 有权

    公开(公告)号：US20220136040A1

    公开(公告)日：2022-05-05

    申请号：US17434065

    申请日：2020-02-26

    申请人： CORNELL UNIVERSITY

    发明人： Roy COHEN ,  Alexander TRAVIS

    IPC分类号： C12Q1/6825 ,  G01N21/76

    摘要： The present application relates to methods of detecting a target nucleic acid molecule in a sample. The method includes providing a sample containing a target nucleic acid molecule and a capture oligonucleotide molecule. In one embodiment, the capture oligonucleotide molecule has (i) a length of 30-60 base pairs, (ii) a 4-8 base pair overhang on its 3′ end, (iii) a 5′ tail, (iv) a target-specific portion between the 3′ end and the 5′ tail, (v) a deoxy-adenosine diphosphate content of 40-50%, (vi) no deoxy thymidine phosphate in the 3′ end or the 5′ tail, and (vii) the 3′ end and the 5′ tail having an ATP content which is 40-50% of that of the capture oligonucleotide molecule. In another aspect of the method of detecting, certain reagents are coupled to a solid support. The present application also relates to compositions and kits useful in carrying out the methods of the present application.