公开(公告)号：US20190177769A1

公开(公告)日：2019-06-13

申请号：US15841006

申请日：2017-12-13

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hatim T. Allawi ,  William G. Weisburg ,  Graham P. Lidgard ,  Michael W. Kaiser ,  Abram M. Vaccaro ,  Gracie Shea

IPC: C12Q1/686 ,  C12N15/65

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

公开(公告)号：US10093987B2

公开(公告)日：2018-10-09

申请号：US15686065

申请日：2017-08-24

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US20180245163A1

公开(公告)日：2018-08-30

申请号：US15906932

申请日：2018-02-27

Applicant: Mayo Foundation for Medical Education and Research ,  EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： David A. Ahlquist ,  William R. Taylor ,  John B. Kisiel ,  Tracy C. Yab ,  Douglas W. Mahoney ,  Brian A. Dukek ,  Matthew T. Gettman ,  Hatim T. Allawi

IPC: C12Q1/6886

Abstract: Provided herein is technology for prostate cancer screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of prostate cancer.

公开(公告)号：US11499179B2

公开(公告)日：2022-11-15

申请号：US16789276

申请日：2020-02-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6886

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US20220136058A1

公开(公告)日：2022-05-05

申请号：US17297356

申请日：2019-11-26

Applicant: Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Maria Giakoumopoulos ,  David A. Ahlquist ,  William R. Taylor ,  Douglas Mahoney

IPC: C12Q1/6886 ,  G01N33/574

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting neoplasms such as lung cancer.

公开(公告)号：US20220106644A1

公开(公告)日：2022-04-07

申请号：US17424422

申请日：2020-01-24

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： William R. Taylor ,  John B. Kisiel ,  Douglas W. Mahoney ,  David A. Ahlquist ,  Hatim T. Allawi ,  Maria Giakoumopoulos

IPC: C12Q1/6886 ,  G16B30/10 ,  G16B35/20

Abstract: Provided herein is technology for endometrial cancer (EC) screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of endometrial cancer and various subtypes of endometrial cancer.

公开(公告)号：US20220071605A1

公开(公告)日：2022-03-10

申请号：US17233199

申请日：2021-04-16

Applicant: Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： Robert F. Eisele ,  Michael E. Williams ,  Prasad G. Iyer ,  Paul Marrione ,  Hatim T. Allawi ,  Graham P. Lidgard ,  Maria Giakoumopoulos ,  William R. Taylor ,  Tracy C. Yab ,  Douglas W. Mahoney ,  John B. Kisiel ,  David A. Ahlquist

IPC: A61B10/02 ,  C12Q1/686 ,  A61B10/00

Abstract: The invention relates to cell sampling devices. In particular, the present invention relates to ingestible cell sampling devices for sampling cells in a subject, and methods of use for detecting abnormalities in a subject using the same. Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer.

公开(公告)号：US20220064740A1

公开(公告)日：2022-03-03

申请号：US17475584

申请日：2021-09-15

Applicant: Exact Sciences Development Company, LLC

Inventor： Manon Van Engeland ,  Manon Adriaan De Bruine ,  Arjan Griffioen ,  Joost Louwagie ,  Katja Bierau ,  Gontran Brichard ,  Gaetan Otto ,  Maarten Penning

IPC: C12Q1/6886

Abstract: A method of detecting a predisposition to, or the incidence of, cancer in a sample comprises detecting an epigenetic change in at least one gene selected from an NDRG4/NDRG2 subfamily gene, GATA4, OSMR, GATA5, SFRP1, ADAM23, JPH3, SFRP2, APC, MGMT, TFPI2, BNIP3, FOXE1, SYNE1, SOX17, PHACTR3 and JAM3, wherein detection of the epigenetic change is indicative of a predisposition to, or the incidence of, cancer. Also described are pharmacogenetic methods for determining suitable treatment regimens for cancer and methods for treating cancer patients, based around selection of the patients according to the methods of the invention. The present invention is also concerned with improved methods of collecting, processing and analyzing samples, in particular body fluid samples. These methods may be useful in diagnosing, staging or otherwise characterizing various diseases. The invention also relates to methods for identifying, diagnosing, staging or otherwise characterizing cancers, in particular gastrointestinal cancers such as colorectal cancers, gastric cancers and oesophageal cancers. The methods of the invention relate, inter alia, to isolating and analyzing the human DNA component from faecal samples and blood-based samples.

公开(公告)号：US20210381066A1

公开(公告)日：2021-12-09

申请号：US17410383

申请日：2021-08-24

Applicant: Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： David A. Ahlquist ,  William R. Taylor ,  John B. Kisiel ,  Douglas W. Mahoney ,  Tracy C. Yab ,  Graham P. Lidgard ,  Hatim T. Allawi

IPC: C12Q1/6886 ,  C12Q1/6883 ,  G01N33/574

Abstract: Provided herein is technology for esophageal disorder screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of esophageal disorders (e.g., Barrett's esophagus, Barrett's esophageal dysplasia, etc.). In addition, the technology provides methods, compositions and related uses for distinguishing between Barrett's esophagus and Barrett's esophageal dysplasia, and between Barrett's esophageal low-grade dysplasia, Barrett's esophageal high-grade dysplasia, and esophageal adenocarcinoma within samples obtained through endoscopic brushing or nonendoscopic whole esophageal brushing or swabbing using a tethered device (e.g. such as a capsule sponge, balloon, or other device).

公开(公告)号：US10975443B2

公开(公告)日：2021-04-13

申请号：US16202935

申请日：2018-11-28

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： David A. Ahlquist ,  William R. Taylor ,  Douglas W. Mahoney ,  Tracy C. Yab ,  John B. Kisiel ,  Hatim T. Allawi ,  Graham P. Lidgard ,  Michael W. Kaiser

IPC: C12Q1/68 ,  C07H21/04 ,  C12Q1/6886 ,  C12Q1/6853 ,  G16H50/30 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/682

Abstract: Provided herein is technology for breast cancer screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of breast cancer.