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165 条记录 (耗时 0.027 秒)

    Methods for forming semiconductor lenses on substrates
    53.
    发明授权
    Methods for forming semiconductor lenses on substrates 有权
    在基片上形成半导体透镜的方法

    公开(公告)号：US06627469B2

    公开(公告)日：2003-09-30

    申请号：US09832413

    申请日：2001-04-10

    申请人： John O'Brien ,  Chuan-cheng Cheng ,  Axel Scherer ,  Amnon Yariv ,  Yong Xu

    发明人： John O'Brien ,  Chuan-cheng Cheng ,  Axel Scherer ,  Amnon Yariv ,  Yong Xu

    IPC分类号： H01L2100

    CPC分类号： H01L33/20

    摘要： A lens is formed out of semiconductor material. The semiconductor produces light which is coupled to the lens. The lens focuses the light and also minimizes refractive reflection. The lens is formed by a graded aluminum alloy, which is oxidized in a lateral direction. The oxidation changes the effective shape of the device according to the grading.

    摘要翻译： 透镜由半导体材料形成。 半导体产生耦合到透镜的光。 镜头对光线进行聚焦，并且还可以减少折射反射。 透镜由横向氧化的渐变铝合金形成。 氧化根据分级改变装置的有效形状。

    Method for cloning and expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli
    55.
    发明授权
    Method for cloning and expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli 有权
    在大肠杆菌中克隆和表达AsiSI限制性内切核酸酶和AsiSI甲基化酶的方法

    公开(公告)号：US06514737B1

    公开(公告)日：2003-02-04

    申请号：US09933313

    申请日：2001-08-20

    申请人： Zhenyu Zhu ,  Shuang-yong Xu

    发明人： Zhenyu Zhu ,  Shuang-yong Xu

    IPC分类号： C12N912

    CPC分类号： C12N9/1007 ,  C12N9/22

    摘要： The present invention relates to recombinant DNA which encodes the AsiSI restriction endonuclease as well as AsiSI methylase, expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli cells containing the recombinant DNA.

    摘要翻译： 本发明涉及编码AsiSI限制性内切核酸酶以及AsiSI甲基化酶，AsiSI限制性内切核酸酶和AsiSI甲基化酶在含有重组DNA的大肠杆菌细胞中的表达的重组DNA。

    Method for cloning and expression of Rhodothermus Obamensis DNA polymerase I large fragment in E. coli
    56.
    发明授权
    Method for cloning and expression of Rhodothermus Obamensis DNA polymerase I large fragment in E. coli 失效
    在大肠杆菌中克隆和表达Rhodothermus Obamensis DNA聚合酶Ⅰ大片段的方法

    公开(公告)号：US06440715B1

    公开(公告)日：2002-08-27

    申请号：US09267311

    申请日：1999-03-12

    申请人： Shuang-yong Xu

    发明人： Shuang-yong Xu

    IPC分类号： C12N912

    CPC分类号： C12N9/1252

    摘要： The present invention provides a novel thermostable DNA polymerase I obtainable from Rhodothermus obamensis, which possesses 3′-5′ exonuclease activity and has a half-life of about 35 minutes at 94° C. This polymerase also contains a tyrosine residue in the ribosome binding site which improves incorporation of dideoxyribonucleic acids. Also provided are isolated DNA and vectors encoding this polymerase, as well as its large fragment, and methods for producing recombinant enzyme using the same.

    摘要翻译： 本发明提供了可从Rhodothermus obamensis获得的新型热稳定DNA聚合酶I，其具有3'-5'核酸外切酶活性，并且在94℃具有约35分钟的半衰期。该聚合酶还在核糖体结合中含有酪氨酸残基 改善二脱氧核糖核酸结合的位点。 还提供了分离的DNA和编码该聚合酶的载体，以及其大片段，以及使用其制备重组酶的方法。

    Method for cloning and expression of Bpml restriction endonuclease in E. coli
    57.
    发明授权
    Method for cloning and expression of Bpml restriction endonuclease in E. coli 有权
    在大肠杆菌中克隆和表达Bpml限制性内切核酸酶的方法

    公开(公告)号：US06413758B1

    公开(公告)日：2002-07-02

    申请号：US09693146

    申请日：2000-10-20

    申请人： Shuang-yong Xu ,  Jian-ping Xiao ,  Zhenyu Zhu

    发明人： Shuang-yong Xu ,  Jian-ping Xiao ,  Zhenyu Zhu

    IPC分类号： C12N922

    CPC分类号： C12N9/22 ,  C07K2319/00 ,  C12N9/1007

    摘要： The present invention relates to recombinant DNA which encodes the BpmI restriction endonuclease as well as BpmI methyltransferase, expression of BpmI restriction endonuclease from E. coli cells containing the recombinant DNA. BpmI endonuclease is a fusion of two distinct elements with a possible structural domains of restriction-methylation-specificity (R-M-S). This domain organization is analogous to the type I restriction-modification system with three distinct subunits, restriction, methylation, and specificity (R, M, and S). Because BpmI is quite distinct to other type IIs restriction enzymes, it is proposed that BpmI belongs to a subgroup of type II restriction enzymes called type IIf (f stands for fusion of restriction-modification-specificity domains). The Type IIf group of restriction enzyme includes Eco57I, BpmI, GsuI, BseRI and some other restriction enzymes that cut downstream sequences at long distance, 10-20 bp downstream of recognition sequence, such as MmeI (N20/N18)).

    摘要翻译： 本发明涉及编码BpmI限制性内切核酸酶以及BpmI甲基转移酶的重组DNA，含有重组DNA的大肠杆菌细胞中BpmI限制性内切核酸酶的表达。 BpmI内切核酸酶是具有限制性甲基化特异性（R-M-S）的可能结构域的两个不同元件的融合物。 这种结构域组织类似于具有三个不同亚基，限制性，甲基化和特异性（R，M和S）的I型限制性修饰系统。 由于BpmI与其他II型限制性内切酶相当不同，所以提出BpmI属于称为IIf型的II型限制酶亚型（f代表限制性修饰特异性结构域的融合）。 IIf类限制酶包括Eco57I，BpmI，GsuI，BseRI和一些其他限制酶，其在识别序列下游10-20bp处长距离切割下游序列，例如MmeI（N20 / N18））。

    Method for cloning and producing Thermomicrobium roseum DNA polymerase I in E. coli
    59.
    发明授权
    Method for cloning and producing Thermomicrobium roseum DNA polymerase I in E. coli 失效
    用于在大肠杆菌中克隆和产生热单孢菌DNA聚合酶I的方法

    公开(公告)号：US5962296A

    公开(公告)日：1999-10-05

    申请号：US28361

    申请日：1998-02-24

    申请人： Laurence Ettwiller ,  Shuang-yong Xu

    发明人： Laurence Ettwiller ,  Shuang-yong Xu

    IPC分类号： C12N9/12 ,  C12N15/54

    CPC分类号： C12N9/1252

    摘要： The present invention relates to isolating DNA coding for DNA polymerase I from Thermomicrobium roseum, expressing the T. roseum DNA polymerase I gene in E. coli and purifying the recombinant T. roseum DNA polymerase I from E. coli cell extract.

    摘要翻译： 本发明涉及从大肠杆菌中分离出来自Thermomicrobium roseum的DNA聚合酶I分离DNA，表达T. roseum DNA聚合酶I基因，从大肠杆菌细胞提取物中纯化重组T. roseum DNA聚合酶I.