公开(公告)号：US11873528B2

公开(公告)日：2024-01-16

申请号：US17943064

申请日：2022-09-12

申请人： 10X GENOMICS, INC.

发明人： Xinying Zheng ,  Serge Saxonov ,  Michael Schnall-Levin ,  Kevin Ness ,  Rajiv Bharadwaj

IPC分类号： C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

CPC分类号： C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2523/101 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2543/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/631 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2523/101 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2543/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/631

摘要： The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

公开(公告)号：US20240002837A1

公开(公告)日：2024-01-04

申请号：US18367638

申请日：2023-09-13

申请人： 10x Genomics, Inc.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC分类号： C12N15/10 ,  C40B20/04 ,  C12Q1/6804 ,  C12Q1/6806 ,  C40B50/16 ,  C12Q1/6874 ,  C12Q1/683

CPC分类号： C12N15/1065 ,  C40B20/04 ,  C12Q1/6804 ,  C12Q1/6806 ,  C40B50/16 ,  C12Q1/6874 ,  C12Q1/683 ,  C12Q2537/143 ,  C12Q2525/191 ,  C12Q2565/629 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2537/149 ,  C12Q2535/122 ,  C12Q2563/149

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11702693B2

公开(公告)日：2023-07-18

申请号：US17152276

申请日：2021-01-19

申请人： 10x Genomics, Inc.

发明人： Rajiv Bharadwaj

IPC分类号： C12Q1/6874

CPC分类号： C12Q1/6874

摘要： This disclosure relates to compositions and methods for analyzing single cells using cell printing and spatial analysis.

公开(公告)号：US20230135010A1

公开(公告)日：2023-05-04

申请号：US17980248

申请日：2022-11-03

申请人： 10x Genomics, Inc.

发明人： Augusto Manuel Tentori ,  Rajiv Bharadwaj

IPC分类号： C12Q1/6837

摘要： Methods of capturing multiple analytes from a biological sample onto a substrate include: (a) positioning a first substrate that includes a plurality of capture probes with respect to a second substrate on which a biological sample that includes multiple analytes is disposed, where the first substrate is permeable; (b) delivering a first fluid to a first surface of the first substrate, where the first surface is opposite to a second surface of the first substrate that faces the second substrate; (c) allowing the first fluid to pass through the first substrate and contact the biological sample; (d) capturing a first one of the multiple analytes with one or more of the capture probes; (e) delivering a second fluid to the first surface of the first substrate; (f) allowing the second fluid to pass through the first substrate and contact the biological sample; and (g) capturing a second one of the multiple analytes with one or more of the capture probes. Other methods of capturing analytes from a biological sample are also described herein.

公开(公告)号：US11565263B2

公开(公告)日：2023-01-31

申请号：US16840619

申请日：2020-04-06

申请人： 10X Genomics, Inc.

发明人： Rajiv Bharadwaj ,  Anthony Makarewicz ,  Bill Kengli Lin

IPC分类号： B01L3/00 ,  G01N1/00 ,  G01N33/543 ,  B01F23/41 ,  B01F25/314 ,  B01F31/80 ,  B01F33/3011 ,  B01F33/302 ,  B01L3/02 ,  C12Q1/6806 ,  G01N33/58 ,  C12Q1/686 ,  C40B40/06

摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20210189475A1

公开(公告)日：2021-06-24

申请号：US17172926

申请日：2021-02-10

申请人： 10x Genomics, Inc.

发明人： Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Felice Alessio Bava

IPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6874

摘要： A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

公开(公告)号：US10583440B2

公开(公告)日：2020-03-10

申请号：US16443629

申请日：2019-06-17

申请人： 10X Genomics, Inc.

发明人： Rajiv Bharadwaj ,  Anthony Makarewicz ,  Bill Kengli Lin

IPC分类号： G01N33/58 ,  G01N33/543 ,  B01L3/02 ,  B01F13/00 ,  B01F5/04 ,  B01F11/02 ,  B01L3/00 ,  B01F3/08 ,  C12Q1/6806 ,  C12Q1/686 ,  C40B40/06

摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US10549279B2

公开(公告)日：2020-02-04

申请号：US15977805

申请日：2018-05-11

申请人： 10X Genomics, Inc.

发明人： Rajiv Bharadwaj ,  Anthony Makarewicz ,  Bill Kengli Lin

IPC分类号： B01L3/00 ,  G01N1/00 ,  B01F5/04 ,  G01N33/543 ,  B01F3/08 ,  B01F13/00 ,  B01F11/02 ,  B01L3/02 ,  C12Q1/6806 ,  G01N33/58 ,  C12Q1/686 ,  C40B40/06

摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190367966A1

公开(公告)日：2019-12-05

申请号：US16375102

申请日：2019-04-04

申请人： 10X Genomics, Inc.

发明人： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC分类号： C12Q1/6806 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6804 ,  G01N33/50 ,  C12N15/10

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.

公开(公告)号：US20190338353A1

公开(公告)日：2019-11-07

申请号：US16426762

申请日：2019-05-30

申请人： 10X GENOMICS, INC.

发明人： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12Q1/683 ,  C12Q1/6806 ,  C12Q1/6804

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.