公开(公告)号：US20240150812A1

公开(公告)日：2024-05-09

申请号：US18388753

申请日：2023-11-10

申请人： 10x Genomics, Inc.

发明人： Felice Alessio Bava ,  Geoffrey McDermott

IPC分类号： C12Q1/6806 ,  C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1013

摘要： Disclosed are methods, compositions and kits for contacting a sample containing a biological particle with a catalyst associated with or attached to a support. The biological particle may be cells and/or nuclei. The catalyst may be an enzyme configured to digest an extracellular molecule, such as an extracellular biological molecule, including extracellular nucleic acid molecules. In some examples, the biological particle is an aggregate of cells that is processed to single cells with a nuclease that is attached to a bead support. The bead and nuclease may subsequently be removed from the system. The single cells that result from the method can be used in single cell-based droplet systems for obtaining genome or transcriptome profiles of single cells.

公开(公告)号：US20240084292A1

公开(公告)日：2024-03-14

申请号：US18355946

申请日：2023-07-20

申请人： 10x Genomics, Inc.

发明人： Zahra Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1068 ,  C12N15/1075 ,  C12N15/1096

摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US11198866B2

公开(公告)日：2021-12-14

申请号：US16206168

申请日：2018-11-30

申请人： 10X Genomics, Inc.

发明人： Zahra Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC分类号： C12N15/10

摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US20210115415A1

公开(公告)日：2021-04-22

申请号：US17114016

申请日：2020-12-07

申请人： 10X Genomics, Inc.

发明人： Josephine Lee ,  Samuel Marrs ,  Geoffrey McDermott ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Joseph Francis Shuga ,  Jessica Michele Terry ,  Solongo B. Ziraldo

IPC分类号： C12N9/12 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6844

摘要： Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.

公开(公告)号：US20210102936A1

公开(公告)日：2021-04-08

申请号：US16897126

申请日：2020-06-09

申请人： 10X Genomics, Inc.

发明人： Zahra Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC分类号： G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20200140944A1

公开(公告)日：2020-05-07

申请号：US16692631

申请日：2019-11-22

申请人： 10X GENOMICS, INC.

发明人： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohamad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20190383798A1

公开(公告)日：2019-12-19

申请号：US16375110

申请日：2019-04-04

申请人： 10X Genomics, Inc.

发明人： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC分类号： G01N33/50 ,  G01N33/543 ,  G01N33/53 ,  G01N33/569 ,  C12N15/10

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

公开(公告)号：US20190153436A1

公开(公告)日：2019-05-23

申请号：US16206168

申请日：2018-11-30

申请人： 10X Genomics, Inc.

发明人： Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC分类号： C12N15/10

摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US11739440B2

公开(公告)日：2023-08-29

申请号：US16897126

申请日：2020-06-09

申请人： 10X Genomics, Inc.

发明人： Jason Bell ,  Geoffrey McDermott ,  Francesca Meschi ,  Katherine Pfeiffer ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： C40B30/04 ,  C12N15/85 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12N15/11 ,  C40B70/00 ,  C40B50/06 ,  C07K14/74 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/569

CPC分类号： C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/505 ,  G01N33/5032 ,  G01N33/532 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/548 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US11725231B2

公开(公告)日：2023-08-15

申请号：US16854475

申请日：2020-04-21

申请人： 10X GENOMICS, INC.

发明人： Geoffrey McDermott

IPC分类号： C12Q1/6806

CPC分类号： C12Q1/6806

摘要： The present disclosure provides methods and systems for nucleic acid preparation and/or analysis. Nucleic acids may be derived from one or more cells. Nucleic acid preparation may comprise generating nucleic acid molecules of varying lengths. Nucleic acid analysis may comprise identifying nucleic acid sequence information with nucleosome position information.