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    Methods for internally controlled in situ assays using padlock probes
    51.
    发明授权
    Methods for internally controlled in situ assays using padlock probes 有权

    公开(公告)号：US12060603B2

    公开(公告)日：2024-08-13

    申请号：US17578296

    申请日：2022-01-18

    Applicant: 10x Genomics, Inc.

    Inventor： Felice Alessio Bava

    IPC: C12P19/34 ,  C12Q1/6827 ,  C12Q1/6841 ,  C12Q1/6853 ,  C12Q1/6876

    CPC classification number: C12Q1/6827 ,  C12Q1/6841 ,  C12Q1/6853 ,  C12Q1/6876

    Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, the methods involve the use of a set of polynucleotides, including one or more polynucleotides (e.g., a detection padlock probe) for detecting a region of interest and one or more polynucleotides (e.g., a control padlock probe) as an internal control, for analyzing target nucleic acids. In some aspects, the presence, amount, and/or identity of a region of interest in a target nucleic acid is analyzed in situ. Also provided are polynucleotides, sets of polynucleotides, compositions, and kits for use in accordance with the methods.

    INCREASING EFFICIENCY OF SPATIAL ANALYSIS IN A BIOLOGICAL SAMPLE
    53.
    发明公开
    INCREASING EFFICIENCY OF SPATIAL ANALYSIS IN A BIOLOGICAL SAMPLE 审中-公开

    公开(公告)号：US20240002931A1

    公开(公告)日：2024-01-04

    申请号：US18340511

    申请日：2023-06-23

    Applicant: 10x Genomics, Inc.

    Inventor： Felice Alessio Bava

    IPC: C12Q1/6874 ,  C12N15/10

    CPC classification number: C12Q1/6874 ,  C12N15/1065

    Abstract: Disclosed herein are methods of amplifying an analyte in a biological sample using a bridging oligonucleotide that hybridizes to a captured analyte. The methods disclosed herein include steps of (a) contacting a biological sample with a substrate having capture probes comprising a capture domain and a spatial barcode; (b) hybridizing the analyte to the capture domain; and (c) contacting the analyte to a bridging oligonucleotide comprising (i) a capture-probe-binding sequence, and (ii) an analyte-binding sequence; (d) extending the bridging oligonucleotide; and (e) determining (i) all or a part of the sequence of the analyte, or a complement thereof, and (ii) the spatial barcode, or a complement thereof, and using the determined sequence of (i) and (ii) to determine the location of the analyte in the biological sample.

    Methods, kits, and compositions for processing extracellular molecules
    54.
    发明授权
    Methods, kits, and compositions for processing extracellular molecules 有权

    公开(公告)号：US11851700B1

    公开(公告)日：2023-12-26

    申请号：US17318364

    申请日：2021-05-12

    Applicant: 10x Genomics, Inc.

    Inventor： Felice Alessio Bava ,  Geoffrey McDermott

    IPC: C12Q1/6806 ,  C12N15/10

    CPC classification number: C12Q1/6806 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q2521/30 ,  C12Q2563/159 ,  C12Q2563/179

    Abstract: Disclosed are methods, compositions and kits for contacting a sample containing a biological particle with a catalyst associated with or attached to a support. The biological particle may be cells and/or nuclei. The catalyst may be an enzyme configured to digest an extracellular molecule, such as an extracellular biological molecule, including extracellular nucleic acid molecules. In some examples, the biological particle is an aggregate of cells that is processed to single cells with a nuclease that is attached to a bead support. The bead and nuclease may subsequently be removed from the system. The single cells that result from the method can be used in single cell-based droplet systems for obtaining genome or transcriptome profiles of single cells.

    IMAGING SYSTEM HARDWARE
    59.
    发明申请
    IMAGING SYSTEM HARDWARE 有权

    公开(公告)号：US20210189475A1

    公开(公告)日：2021-06-24

    申请号：US17172926

    申请日：2021-02-10

    Applicant: 10x Genomics, Inc.

    Inventor： Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Felice Alessio Bava

    IPC: C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6874

    Abstract: A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

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