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    Assays for the presence of a mutant EGF receptor
    53.
    发明授权
    Assays for the presence of a mutant EGF receptor 失效
    测定突变EGF受体的存在

    公开(公告)号：US5710010A

    公开(公告)日：1998-01-20

    申请号：US459673

    申请日：1995-06-02

    申请人： Bert Vogelstein ,  Darell Bigner

    发明人： Bert Vogelstein ,  Darell Bigner

    IPC分类号： A61K39/395 ,  A61K38/00 ,  A61K45/00 ,  A61K47/48 ,  A61K49/00 ,  A61K51/10 ,  A61P35/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/71 ,  C07K16/00 ,  C07K16/28 ,  C12N1/21 ,  C12N5/10 ,  C12N15/02 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/574 ,  G01N33/577 ,  G01N33/532 ,  C07K15/28

    CPC分类号： C07K14/71 ,  A61K47/48561 ,  A61K51/103 ,  C07K16/2863 ,  C12Q1/6886 ,  A61K2123/00 ,  A61K38/00 ,  C07K2317/34 ,  C12Q2600/156 ,  Y10S435/96 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S530/81 ,  Y10S530/812

    摘要： The epidermal growth factor receptor (EGFR) gene is amplified in 40% of malignant gliomas and the amplified genes are frequently rearranged. The genetic alterations associated with these rearrangements are characterized in five malignant gliomas. In one tumor, the rearrangement resulted in the deletion of most of the extracytoplasmic domain of the receptor, resulting in a hybrid mRNA between new sequences and the truncated EGFR. The predicted amino acid sequence of the protein from this tumor was remarkably similar to that described for several viral erb-B oncogenes. Four other tumors were noted to have internal deletions of the EGF receptor gene. These rearrangements brought about in-frame deletions affecting either of two cysteine-rich domains in the extracytoplasmic portion of the molecule. The clonal nature of these alterations, and the fact that identical alterations were seen in more than one tumor, suggests a role for these mutant receptor proteins in tumorigenesis. Furthermore, these studies document the existence of tumor specific cell molecules resulting from somatic mutation.

    摘要翻译： 在40％恶性胶质瘤中扩增表皮生长因子受体（EGFR）基因，经扩增的基因经常重排。 与这些重排相关的遗传改变的特征在于五种恶性胶质瘤。 在一个肿瘤中，重排导致大部分受体胞外结构域的缺失，导致新序列与截短的EGFR之间的杂合mRNA。 来自该肿瘤的蛋白质的预测氨基酸序列与对于几种病毒erb-B致癌基因所描述的非常相似。 注意到另外四个肿瘤具有EGF受体基因的内部缺失。 这些重排导致了在分子的胞质内部分中影响两个富含半胱氨酸的结构域的框内缺失。 这些改变的克隆性质以及在多于一种肿瘤中观察到相同改变的事实表明这些突变受体蛋白在肿瘤发生中的作用。 此外，这些研究记录了由体细胞突变引起的肿瘤特异性细胞分子的存在。

    HUMAN TRANSCRIPTOMES
    57.
    发明申请
    HUMAN TRANSCRIPTOMES 审中-公开
    人物代码

    公开(公告)号：US20110033466A1

    公开(公告)日：2011-02-10

    申请号：US12858717

    申请日：2010-08-18

    申请人： Victor E. Velculescu ,  Bert Vogelstein ,  Kenneth W. Kinzler

    发明人： Victor E. Velculescu ,  Bert Vogelstein ,  Kenneth W. Kinzler

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12N5/071 ,  C12N15/00 ,  A61K31/7088 ,  A61K39/395 ,  A61P35/00 ,  A61P43/00

    CPC分类号： C12Q1/6886 ,  C12Q2600/136

    摘要： Global gene expression patterns have been characterized in normal and cancerous human cells using serial analysis of gene expression (SAGE). Cancer cell-specific, cell-type specific, and ubiquitously expressed genes have been identified. This information can be used to provide combinations of cell type- and cancer-specific gene probes, as well as methods of using these probes to identify particular cell types, screen for useful drugs, reduce cancer-specific gene expression, standardize gene expression, and restore function to a diseased cell or tissue.

    摘要翻译： 使用基因表达（SAGE）的连续分析，在正常和癌性人类细胞中表征全球基因表达模式。 已经鉴定了癌细胞特异性，细胞型特异性和普遍表达的基因。 该信息可用于提供细胞类型和癌症特异性基因探针的组合，以及使用这些探针来鉴定特定细胞类型，筛选有用药物，降低癌症特异性基因表达，标准化基因表达的方法，以及 恢复功能到病变细胞或组织。

    Phosphatase associated with metastasis
    58.
    发明授权
    Phosphatase associated with metastasis 有权
    与转移相关的磷酸酶

    公开(公告)号：US07745165B2

    公开(公告)日：2010-06-29

    申请号：US10868658

    申请日：2004-06-16

    申请人： Bert Vogelstein ,  Kenneth W. Kinzler ,  Saurabh Saha ,  Alberto Bardelli

    发明人： Bert Vogelstein ,  Kenneth W. Kinzler ,  Saurabh Saha ,  Alberto Bardelli

    IPC分类号： C12Q1/48 ,  G01N33/567

    CPC分类号： C12N9/16 ,  A61K39/00 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/136 ,  C12Q2600/158

    摘要： Among the genes identified, in a comparison of the global gene expression profile of metastatic colorectal cancer to that of primary cancers, benign colorectal tumors, and normal colorectal epithelium, the PRL-3 protein tyrosine phosphatase gene was of particular interest. It was expressed at high levels in each of 18 cancer metastases studied but at lower levels in non-metastatic tumors and normal colorectal epithelium. In three of twelve metastases examined, multiple copies of the PRL-3 gene were found within a small amplicon located at chromosome 8q24.3. These data suggest that the PRL-3 gene is important for colorectal cancer metastasis and provides a new therapeutic target for these intractable lesions.

    摘要翻译： 在确定的基因中，在转移性结肠直肠癌与原发性癌症，良性结肠直肠肿瘤和正常结肠直肠上皮的全球基因表达谱比较中，PRL-3蛋白酪氨酸磷酸酶基因特别引人关注。 在所研究的18个癌症转移中的每一个中表达高水平，但在非转移性肿瘤和正常结肠直肠上皮中的水平较低。 在检查的十二个转移中的三个中，在位于染色体8q24.3的小扩增子中发现了PRL-3基因的多个拷贝。 这些数据表明，PRL-3基因对结肠直肠癌转移很重要，为这些难治性病变提供了新的治疗靶点。

    Methods for generating hypermutable yeast
    59.
    发明授权
    Methods for generating hypermutable yeast 有权
    产生超可变酵母的方法

    公开(公告)号：US07514216B2

    公开(公告)日：2009-04-07

    申请号：US11188743

    申请日：2005-07-26

    申请人： Nicholas C. Nicolaides ,  Philip M. Sass ,  Luigi Grasso ,  Bert Vogelstein ,  Kenneth W. Kinzler

    发明人： Nicholas C. Nicolaides ,  Philip M. Sass ,  Luigi Grasso ,  Bert Vogelstein ,  Kenneth W. Kinzler

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/81

    摘要： Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.

    摘要翻译： 酵母细胞被诱变以获得所需的突变体。 诱变由缺陷错配修复系统介导，可以使用常规的外源应用诱变剂进行增强。 具有缺陷错配修复系统的酵母细胞是可超过可变的，但是在选择所需的突变酵母菌株之后，可以通过将错配修复系统恢复到适当的功能来使它们变得遗传稳定。

    Endothelial Cell Expression Patterns
    60.
    发明申请
    Endothelial Cell Expression Patterns 审中-公开
    内皮细胞表达模式

    公开(公告)号：US20090017030A1

    公开(公告)日：2009-01-15

    申请号：US11930528

    申请日：2007-10-31

    申请人： Brad St.Croix ,  Bert Vogelstein ,  Kenneth W. Kinzler

    发明人： Brad St.Croix ,  Bert Vogelstein ,  Kenneth W. Kinzler

    IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N5/00 ,  G01N33/567 ,  A61K38/16 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/435 ,  A61K39/00 ,  C07K17/00

    CPC分类号： C07K16/30 ,  A61K2039/505

    摘要： To gain a better understanding of tumor angiogenesis, new techniques for isolating endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, over 170 genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed 79 differentially expressed genes, including 46 that were specifically elevated in tumor-associated endothelium. Experiments with representative genes from this group demonstrated that most were similarly expressed in the endothelium of primary lung, breast, brain, and pancreatic cancers as well as in metastatic lesions of the liver. These results demonstrate that neoplastic and normal endothelium in humans are distinct at the molecular level, and have significant implications for the development of anti-angiogenic therapies in the future.

    摘要翻译： 为了更好地了解肿瘤血管生成，开发了分离内皮细胞（ECs）和评估基因表达模式的新技术。 将来自正常和恶性结肠直肠组织的ECs的转录物与来自非内皮细胞的转录物进行比较，鉴定了主要在内皮中表达的170个以上的基因。 正常和肿瘤来源的内皮的比较显示79个差异表达基因，其中46个在肿瘤相关内皮特异性升高。 来自该组的代表性基因的实验表明，大多数类似物在原发性肺癌，乳腺癌，脑癌和胰腺癌的内皮以及肝转移性损伤中都表达。 这些结果表明，人类的肿瘤和正常内皮在分子水平上是不同的，对未来抗血管生成疗法的发展具有重要意义。