METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF ORGANIC SUBSTANCES FROM FERMENTING MICROORGANISMS
    51.
    发明申请
    METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF ORGANIC SUBSTANCES FROM FERMENTING MICROORGANISMS 审中-公开
    从生物微生物增强生物有机物质的方法和组合物

    公开(公告)号:US20140287473A1

    公开(公告)日:2014-09-25

    申请号:US13848950

    申请日:2013-03-22

    IPC分类号: C12P7/10 C12P19/14

    摘要: The present invention relates methods and compositions for producing an organic substance from fermenting microorganism using simultaneous saccharification and fermentation. One aspect of the invention provides producing alcohol by simultaneous saccharification and fermentation by combining a cellulosic substrate, and whole fermentation broth, and an ethanologenic microorganism under conditions conducive both to hydrolysis of cellulose to glucose and to conversion of glucose to alcohol.

    摘要翻译: 本发明涉及使用同时糖化和发酵从发酵微生物生产有机物质的方法和组合物。 本发明的一个方面通过在有利于纤维素水解成葡萄糖和将葡萄糖转化成醇的条件下,通过将纤维素底物和全发酵肉汤以及酿酒酵母同时进行糖化和发酵来生产醇。

    Oxidatively stable alpha-amylase
    57.
    发明授权
    Oxidatively stable alpha-amylase 失效
    氧化稳定的α-淀粉酶

    公开(公告)号:US5849549A

    公开(公告)日:1998-12-15

    申请号:US468698

    申请日:1995-06-06

    摘要: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

    摘要翻译: 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常,通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代(置换)或缺失,获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比,这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物,以及使用突变淀粉酶的方法。

    Method for liquefying starch
    58.
    发明授权
    Method for liquefying starch 失效
    液化淀粉的方法

    公开(公告)号:US5652127A

    公开(公告)日:1997-07-29

    申请号:US459984

    申请日:1995-06-02

    CPC分类号: C12P19/14 C08B30/12

    摘要: According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.

    摘要翻译: 根据本发明,提供了一种用于液化淀粉的方法,包括以下步骤:在淀粉液化之前或同时将淀粉钠加入淀粉中; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。 优选的钠组合物包括氯化钠,碳酸氢钠,苯甲酸钠,硫酸钠,亚硫酸氢钠,抗坏血酸钠,乙酸钠,硝酸钠,酒石酸钠,四硼酸钠,丙酸钠,柠檬酸钠,琥珀酸钠,谷氨酸钠,柠檬酸三钠, 磷酸盐或其混合物。

    USE OF CELLULASE AND GLUCOAMYLASE TO IMPROVE ETHANOL YIELDS FROM FERMENTATION
    59.
    发明申请
    USE OF CELLULASE AND GLUCOAMYLASE TO IMPROVE ETHANOL YIELDS FROM FERMENTATION 审中-公开
    纤维素酶和谷氨酰胺酶从发酵中提高乙醇含量

    公开(公告)号:US20120276593A1

    公开(公告)日:2012-11-01

    申请号:US13458597

    申请日:2012-04-27

    摘要: An improved saccharification process comprises the use of a glucoamylase and at least one cellulase. The improved saccharification process results in improved yields of fermentations products, such as ethanol. In one embodiment, the improved saccharification process results in an increased yield of up to 0.5% to 1% ethanol using commercially available cellulases. Also provided are improved simultaneous saccharification and fermentation (SSF) processes, and compositions comprising a liquefied starch slurry, a glucoamylase, and a cellulase.

    摘要翻译: 改进的糖化方法包括使用葡​​糖淀粉酶和至少一种纤维素酶。 改进的糖化过程导致发酵产物如乙醇的产率提高。 在一个实施方案中,改进的糖化过程导致使用市售的纤维素酶增加高达0.5%至1%乙醇的产量。 还提供了改进的同时糖化和发酵(SSF)方法,以及包含液化淀粉浆料,葡糖淀粉酶和纤维素酶的组合物。