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    Oxidatively stable alpha-amylase
    2.
    发明授权
    Oxidatively stable alpha-amylase 失效
    氧化稳定的α-淀粉酶

    公开(公告)号：US5849549A

    公开(公告)日：1998-12-15

    申请号：US468698

    申请日：1995-06-06

    申请人： Christopher C. Barnett ,  Leif P. Solheim ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

    发明人： Christopher C. Barnett ,  Leif P. Solheim ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N15/56 ,  C12N15/75 ,  C12P19/14

    CPC分类号： C12N9/2417 ,  C11D3/386 ,  C12N15/75 ,  C12P19/14

    摘要： Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

    摘要翻译： 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。

    Method for liquefying starch
    3.
    发明授权
    Method for liquefying starch 失效
    液化淀粉的方法

    公开(公告)号：US5652127A

    公开(公告)日：1997-07-29

    申请号：US459984

    申请日：1995-06-02

    申请人： Colin Mitchinson ,  Leif P. Solheim

    发明人： Colin Mitchinson ,  Leif P. Solheim

    IPC分类号： C08B30/12 ,  C12P19/14 ,  C12M9/24 ,  C12M9/26

    CPC分类号： C12P19/14 ,  C08B30/12

    摘要： According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.

    摘要翻译： 根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时将淀粉钠加入淀粉中; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。 优选的钠组合物包括氯化钠，碳酸氢钠，苯甲酸钠，硫酸钠，亚硫酸氢钠，抗坏血酸钠，乙酸钠，硝酸钠，酒石酸钠，四硼酸钠，丙酸钠，柠檬酸钠，琥珀酸钠，谷氨酸钠，柠檬酸三钠， 磷酸盐或其混合物。

    Method for liquefying starch
    4.
    发明授权
    Method for liquefying starch 失效
    液化淀粉的方法

    公开(公告)号：US5756714A

    公开(公告)日：1998-05-26

    申请号：US411038

    申请日：1995-03-27

    申请人： Richard L. Antrim ,  Colin Mitchinson ,  Leif P. Solheim

    发明人： Richard L. Antrim ,  Colin Mitchinson ,  Leif P. Solheim

    IPC分类号： C08B30/12 ,  C12P19/14 ,  A23J1/14 ,  A23L1/211

    CPC分类号： C08B30/12 ,  C12P19/14

    摘要： According to the invention a method is provided for liquefying starch comprising the steps of treating the starch prior to or simultaneously with liquefying the starch to inactivate and/or remove the enzyme inhibiting composition present in the starch and form treated starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Effective means to treat the starch include the addition of a phytate degrading enzyme and heat treatment, optionally followed by filtration or centrifugation, of granular starch or a starch solution.

    摘要翻译： 根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时处理淀粉以灭活和/或除去淀粉中存在的酶抑制组合物并形成经处理的淀粉; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。 处理淀粉的有效方法包括加入植酸盐降解酶和热处理，任选地随后过滤或离心淀粉或淀粉溶液。

    Method for liquefying starch
    5.
    发明授权
    Method for liquefying starch 失效
    液化淀粉的方法

    公开(公告)号：US06184002B2

    公开(公告)日：2001-02-06

    申请号：US08952225

    申请日：1998-01-27

    申请人： Colin Mitchinson ,  Leif P. Solheim

    发明人： Colin Mitchinson ,  Leif P. Solheim

    IPC分类号： C12P1914

    CPC分类号： C12P19/14 ,  C08B30/12

    摘要： According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding &agr;-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.

    摘要翻译： 根据本发明，提供了一种用于液化淀粉的方法，包括以下步骤：在淀粉液化之前或同时将淀粉钠加入淀粉中; 向经处理的淀粉中加入α-淀粉酶; 并使经处理的淀粉一段时间和在有效液化经处理的淀粉的温度下反应。 优选的钠组合物包括氯化钠，碳酸氢钠，苯甲酸钠，硫酸钠，亚硫酸氢钠，抗坏血酸钠，乙酸钠，硝酸钠，酒石酸钠，四硼酸钠，丙酸钠，柠檬酸钠，琥珀酸钠，谷氨酸钠，柠檬酸三钠， 磷酸盐或其混合物。