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公开(公告)号：US07550259B2
公开(公告)日：2009-06-23
申请号：US11484912
申请日：2006-07-12
IPC分类号： C12Q1/00
CPC分类号： G01N33/6848 , B01L3/5027 , G01N27/447 , G01N30/7233 , G01N33/6851 , H01J49/04
摘要： A method of analyzing a sample comprising multiple protein species is provided. The proteins are separated by species such that the multiple protein species emerge in a sequential order and are then digested in the sequential order in which they emerge from the separation process. The digested proteins are introduced into a mass spectrometer in the same sequential order so that, within a given time window, the digested proteins introduced into the mass spectrometer are covariant.
摘要翻译： 提供了分析包含多种蛋白质种类的样品的方法。 蛋白质被物种分离，使得多个蛋白质物质以顺序排列出来，然后以从分离过程中出现的顺序消化。 将消化的蛋白质以相同的顺序引入质谱仪，使得在给定的时间窗口内，引入质谱仪的消化蛋白质是共变的。
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公开(公告)号：US07368708B2
公开(公告)日：2008-05-06
申请号：US11399778
申请日：2006-04-07
申请人： Steven M. Fischer
发明人： Steven M. Fischer
CPC分类号： H01T23/00 , H01J49/045 , H01J49/165
摘要： The present invention relates to an apparatus for producing ions from an electrospray assembly. The apparatus comprises an ionization chamber, an electrospray assembly, an isolating electrode, an isolated ionization region within the ionization chamber and optionally, a vacuum interface at a vacuum interface voltage and a vacuum chamber. Sample is introduced into the ionization chamber from an electrospray assembly and transported to an electric field region that is isolated from the electric field created by the electrospray assembly.
摘要翻译： 本发明涉及一种用于从电喷雾组件产生离子的装置。 该装置包括电离室，电喷雾组件，隔离电极，离子化室内的隔离电离区域，以及真空界面电压和真空室中的真空界面。 样品从电喷雾组件引入电离室，并被传送到与由电喷雾组件产生的电场隔离的电场区域。
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53.发明授权Ambient pressure matrix-assisted laser desorption ionization (MALDI) apparatus and method of analysis 有权环境压力矩阵辅助激光解吸电离（MALDI）装置及分析方法公开(公告)号：US07193206B2
公开(公告)日：2007-03-20
申请号：US10806907
申请日：2004-03-22
申请人： Jian Bai , Steven M. Fischer , J. Michael Flanagan
发明人： Jian Bai , Steven M. Fischer , J. Michael Flanagan
CPC分类号： H01J49/164
摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises: The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.
摘要翻译： 公开了具有在环境压力下操作的基质辅助激光解吸电离（MALDI）源的质谱仪。 公开了该装置和方法以使用矩阵辅助激光解吸电离（MALDI）分析至少一个含有至少一种分析物的样品，该装置包括：本发明涉及用于将至少一种分析物离子化的装置和方法 用于传送到质量分析装置的样品，包括：（a）电离外壳，其包括经配置以将离子输送到质量分析装置的通道; （b）将所述电离外壳保持在大于100mTorr的环境压力下的装置; （c）构造成用于在所述环境压力下将包含所述样品的基质保持在所述电离外壳中的保持器; （d）激光能量源，其包括与电离外壳相关联的装置，用于将激光能量引导到由保持器在环境压力下维持的所述基体上以解吸并离子化样品中的至少一部分分析物，以及（e） 用于将所述至少一种离子化分析物的至少一部分引导到所述通道中的装置。 环境压力（AP-MALDI）源与各种质量分析仪兼容，特别是与质谱仪兼容，解决了与在真空下运行的常规MALDI源有关的许多问题。 描述了大气压力MALDI。 描述了有机分子或其片段，特别是生物分子，例如生物聚合物和生物体的分析。
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公开(公告)号：US07180059B2
公开(公告)日：2007-02-20
申请号：US11471954
申请日：2006-06-21
IPC分类号： H01J49/10
CPC分类号： H01J49/04 , H01J49/10 , H01J49/107 , H01J49/165
摘要： The present invention relates to an apparatus and method for use with a mass spectrometry system. The invention provides an ion source, infrared emitter and sensor with closed control feedback loop coupled to the infrared emitter. Methods of control and heating using the apparatus of the present invention are also disclosed.
摘要翻译： 本发明涉及一种与质谱系统一起使用的装置和方法。 本发明提供一种离子源，红外发射器和传感器，其具有耦合到红外发射器的闭合控制反馈环路。 还公开了使用本发明的设备进行控制和加热的方法。
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公开(公告)号：US07112786B2
公开(公告)日：2006-09-26
申请号：US11168756
申请日：2005-06-27
IPC分类号： H01J49/04
CPC分类号： H01J49/061 , H01J49/04 , H01J49/10
摘要： For generation and delivery of ions from an ionization chamber through an ion entrance orifice to a mass analyzer operating at high vacuum, high pass ion filtration is effected within the ionization chamber by application of electrical potentials to an electrode associated with the ion entrance orifice and to an electrode between the ionization region and the ion entrance orifice to create a retarding electric field upstream from the ion entrance orifice. The retarding electric field hinders the movement to the ion entrance orifice of ions having drift velocities below a lower limit, and as the retarding voltage gradient is made steeper, the lower limit increases.
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56.发明授权公开(公告)号：US07102128B2
公开(公告)日：2006-09-05
申请号：US10806808
申请日：2004-03-22
申请人： Jian Bai , Steven M. Fischer , J. Michael Flanagan
发明人： Jian Bai , Steven M. Fischer , J. Michael Flanagan
CPC分类号： H01J49/164
摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.
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公开(公告)号：US07041966B2
公开(公告)日：2006-05-09
申请号：US10977179
申请日：2004-10-29
CPC分类号： H01J49/165 , H01J49/045
摘要： The present invention relates to an apparatus for delivering ions to a vacuum chamber. The apparatus comprises an ionization chamber, an ionization region within the ionization chamber, a vacuum interface at a vacuum interface voltage and a vacuum chamber, wherein the ionization chamber communicates with the vacuum chamber through the vacuum interface. Sample is introduced into the ionization chamber from an electrospray assembly at approximately ground potential. Two electrodes are provided within the chamber such that three electric fields are generated, a first field extending from the electrospray assembly to the first electrode, a second field extending from the second electrode to the first electrode, and a third field extending from the second electrode to the vacuum interface. Ions are forced to travel through the fields in order before entering the vacuum chamber. In addition, the invention provides a method of delivering ions to a vacuum chamber.
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公开(公告)号：US06613523B2
公开(公告)日：2003-09-02
申请号：US09896299
申请日：2001-06-29
申请人： Steven M. Fischer
发明人： Steven M. Fischer
IPC分类号： C12Q168
CPC分类号： C12Q1/6869 , C12Q2525/186 , C12Q2523/319
摘要： The present invention provides novel systems for sequencing nucleic acid molecules using dNTPs that are 3′ end labeled with cleavable tags that block further extension and uniquely identify the bases to which they are attached. Removal of the tags liberates the 3′ ends of the extension products for further extension. In related embodiments, oligonucleotides containing sequence-related cleavable tags are employed in a ligation reaction to determine the sequence of a particular DNA sample.
摘要翻译： 本发明提供了使用具有3'末端标记的可切割标签的dNTP来测序核酸分子的新型系统，其可以阻断进一步的扩增并且唯一地鉴定它们所连接的碱基。 移除标签可以扩展扩展产品的3'端，进一步扩展。 在相关实施方案中，在连接反应中使用含有序列相关可切割标签的寡核苷酸以确定特定DNA样品的序列。
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公开(公告)号：US06406633B1
公开(公告)日：2002-06-18
申请号：US09611148
申请日：2000-07-06
申请人： Steven M. Fischer , Glen F. Ingle
发明人： Steven M. Fischer , Glen F. Ingle
IPC分类号： B01D1508
CPC分类号： G01N30/82 , G01N30/7233 , G01N30/62 , G01N30/84 , G01N30/10
摘要： A fraction collection system for liquid chromatography includes a conduit, which directs a sample eluent from an LC column through a flow splitter to a destructive analytical detector such as a mass spectrometer to analyze sample components, and to a fraction collector. A nondestructive detector such as a photodetector is positioned near the fraction collector to detect arrival of sample components. Optionally, a nondestructive detector such as a photodetector is situated upstream from the splitter, to detect passing sample components. Time intervals between detection of a calibrant or a sample component at any two of the detectors provide for automatable identification of selected sample components at the sample collector.
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