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    Image forming and erasing apparatus and image forming and erasing method
    51.
    发明授权
    Image forming and erasing apparatus and image forming and erasing method 有权
    图像形成和擦除装置及图像形成和擦除方法

    公开(公告)号：US08879976B2

    公开(公告)日：2014-11-04

    申请号：US13291338

    申请日：2011-11-08

    申请人： Shigeru Fujiwara ,  Satoshi Kinouchi ,  Ryota Saeki ,  Shoko Shimmura ,  Kazutoshi Takahashi ,  Toshihiro Sone ,  Takafumi Amano

    发明人： Shigeru Fujiwara ,  Satoshi Kinouchi ,  Ryota Saeki ,  Shoko Shimmura ,  Kazutoshi Takahashi ,  Toshihiro Sone ,  Takafumi Amano

    IPC分类号： G03G15/20 ,  G03G15/00

    CPC分类号： G03G15/6573 ,  G03G15/6585 ,  G03G15/6591

    摘要： An image forming apparatus according to an embodiment includes: an image erasing section including a first member which has an elastic layer and heats a recording medium having a thermally decolorizable toner image on the toner image side and a second member which erases the toner image by allowing the recording medium in cooperation with the first member; an image forming section which transfers a thermally decolorizable toner image onto the recording medium on which the toner image is erased to form an unfixed toner image; and an image fixing section including a third member which does not have an elastic layer and heats the recording medium on the unfixed toner image side and a fourth member which fixes the unfixed toner image by allowing the recording medium in cooperation with the third member.

    摘要翻译： 根据实施例的图像形成装置包括：图像擦除部分，包括具有弹性层的第一部件，并且在调色剂图像侧加热具有热可脱色调色剂图像的记录介质，以及通过允许调色剂图像来消除调色剂图像的第二部件 记录介质与第一个成员协作; 将热可脱色调色剂图像转印到其上清除调色剂图像的记录介质上以形成未定影的调色剂图像的图像形成部分; 以及图像定影部，其包括不具有弹性层的第三构件，并且对未定影的调色剂像侧加热记录介质;以及第四构件，其通过允许记录介质与第三构件协作来固定未定影的调色剂图像。

    EFFICIENT METHOD FOR ESTABLISHING INDUCED PLURIPOTENT STEM CELLS
    54.
    发明申请
    EFFICIENT METHOD FOR ESTABLISHING INDUCED PLURIPOTENT STEM CELLS 有权
    建立诱导型脑干细胞的有效方法

    公开(公告)号：US20130267030A1

    公开(公告)日：2013-10-10

    申请号：US13991341

    申请日：2011-12-02

    申请人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Koji Tanabe

    发明人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Koji Tanabe

    IPC分类号： C12N5/071 ,  C12N15/85

    CPC分类号： C12N5/0696 ,  C12N15/85 ,  C12N2501/115 ,  C12N2501/415 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/998 ,  C12N2506/1307 ,  C12N2510/00

    摘要： The invention provides a method of improving the efficiency of establishment of induced pluripotent stem cells by increasing, in a nuclear reprogramming step of somatic cell, the level of activated form of one or more proteins selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K. The invention also provides a method of producing induced pluripotent stem cells by contacting a somatic cell with a nuclear reprogramming substance and one or more of such proteins and nucleic acids that encode such proteins. The invention further provides an induced pluripotent stem cell that has an exogenous nucleic acid encoding such a protein, as well as agents for use in the aforesaid methods.

    摘要翻译： 本发明提供了一种通过在体细胞核重编程步骤中增加一种或多种蛋白质的活化形式的水平来提高诱导多能干细胞的建立效率的方法，该蛋白质选自Ras家族成员，PI3激酶 ，RalGEF，Raf，AKT家族成员，Rheb，TCL1和S6K。 本发明还提供了通过使体细胞与核重编程物质和一种或多种这样的蛋白质和编码这种蛋白质的核酸接触来产生诱导性多能干细胞的方法。 本发明还提供了具有编码这种蛋白质的外源核酸的诱导多能干细胞，以及用于上述方法的试剂。

    Method for preparation of platelet from iPS cell
    55.
    发明授权
    Method for preparation of platelet from iPS cell 有权
    从iPS细胞制备血小板的方法

    公开(公告)号：US08546141B2

    公开(公告)日：2013-10-01

    申请号：US12935380

    申请日：2009-04-01

    申请人： Hiromitsu Nakauchi ,  Koji Eto ,  Hidekazu Nishiki-I ,  Naoya Takayama ,  Shinya Yamanaka ,  Kazutoshi Takahashi

    发明人： Hiromitsu Nakauchi ,  Koji Eto ,  Hidekazu Nishiki-I ,  Naoya Takayama ,  Shinya Yamanaka ,  Kazutoshi Takahashi

    IPC分类号： C12N5/00 ,  C12N15/00

    CPC分类号： C12N5/0644 ,  A61K35/12 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/165 ,  C12N2501/91 ,  C12N2502/1394 ,  C12N2506/45

    摘要： A method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells is achieved in an in vitro culture system. A sac-like structure encloses hematopoietic progenitor cells, which is obtained by inoculating iPS cells onto feeder cells and then culturing the iPS cells under conditions suitable for inducing the differentiation of hematopoietic progenitor cells. Moreover, a method for producing various types of blood cells, comprises culturing hematopoietic progenitor cells enclosed in the sac-like structure under conditions suitable for inducing the differentiation of blood cells. Furthermore, a method for producing various types of blood cells, particularly megakaryocytes and platelets, is achieved without involving the sac-like structure.

    摘要翻译： 在体外培养系统中实现了从iPS细胞中有效地制备血细胞如成熟巨核细胞和血小板的方法。 囊状结构包围造血祖细胞，其通过将iPS细胞接种在饲养细胞上，然后在适于诱导造血祖细胞分化的条件下培养iPS细胞而获得。 此外，生产各种血细胞的方法包括在适于诱导血细胞分化的条件下培养包封在囊样结构中的造血祖细胞。 此外，不涉及囊状结构，可以实现生产各种血细胞，特别是巨核细胞和血小板的方法。

    Method of efficiently establishing induced pluripotent stem cells
    56.
    发明授权
    Method of efficiently establishing induced pluripotent stem cells 有权
    有效建立诱导性多能干细胞的方法

    公开(公告)号：US08530238B2

    公开(公告)日：2013-09-10

    申请号：US12672042

    申请日：2009-06-26

    申请人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Keisuke Okita

    发明人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Keisuke Okita

    IPC分类号： C12N15/00 ,  C12N5/00

    CPC分类号： C12N5/0696 ,  C12N15/85 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/11 ,  C12N2510/00

    摘要： The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells, comprising inhibiting the p53 function in the step of somatic cell nuclear reprogramming. The inhibition of p53 function is achieved by bringing a substance selected from the group consisting of (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors, into contact with a somatic cell, and the like. The present invention also provides an agent for improving the efficiency of establishment of iPS cells, the agent comprising an inhibitor of p53 function, particularly (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors. The present invention further provides a method of producing an iPS cell, comprising bringing a nuclear reprogramming substance and an inhibitor of p53 function into contact with a somatic cell.

    摘要翻译： 本发明提供了提高诱导多能干细胞（iPS）细胞的建立效率的方法，包括抑制体细胞核重编程步骤中的p53功能。 通过使选自（1）p53的化学抑制剂，（2）p53的显性失活突变体和编码其的核酸，（3）siRNA和shRNA的物质与p53和 编码相同的DNA，（4）p53途径抑制剂，与体细胞接触等。 本发明还提供了用于提高iPS细胞的建立效率的试剂，该试剂包含p53功能的抑制剂，特别是（1）p53的化学抑制剂，（2）p53的显性失活突变体和编码其的核酸 ，（3）针对p53的siRNA和shRNA，编码相同的DNA，以及（4）p53途径抑制剂。 本发明还提供了一种制备iPS细胞的方法，包括使核重编程物质和p53功能抑制剂与体细胞接触。

    IMAGE FORMING METHOD
    57.
    发明申请
    IMAGE FORMING METHOD 审中-公开
    图像形成方法

    公开(公告)号：US20130164008A1

    公开(公告)日：2013-06-27

    申请号：US13406858

    申请日：2012-02-28

    申请人： Shoko Shimmura ,  Satoshi Kinouchi ,  Kazutoshi Takahashi ,  Toshihiro Sone ,  Ryota Saeki

    发明人： Shoko Shimmura ,  Satoshi Kinouchi ,  Kazutoshi Takahashi ,  Toshihiro Sone ,  Ryota Saeki

    IPC分类号： G03G15/01 ,  G03G15/08

    CPC分类号： G03G13/08 ,  G03G15/6585 ,  G03G21/00

    摘要： According to one embodiment, a developing device in which a decolorizable developing agent containing encapsulated colorant microparticles each including a core containing a color developable compound, a color developer, and a decolorizing agent is accommodated and a developing device in which a transparent developing agent containing a binder resin is accommodated are provided, respectively, and an image is formed by transferring a transparent developing agent image onto a region capable of covering a decolorizable developing agent image, followed by fusing the image. Further, in the second image formation and thereafter, the using amount of the transparent developing agent is decreased as compared with that in the first image formation.

    摘要翻译： 根据一个实施方案，一种显影装置，其中包含含有包含可显色化合物的核心的着色着色剂微粒的可脱色显影剂，显色剂和脱色剂，以及显影装置，其中含有 分别设置粘合剂树脂，并且通过将透明显影剂图像转印到能够覆盖可消色显影剂图像的区域上，然后使图像融合来形成图像。 此外，在第二图像形成中，此后，与第一图像形成相比，透明显影剂的使用量减少。

    METHOD FOR SELECTING HUMAN INDUCED PLURIPOTENT STEM CELLS
    58.
    发明申请
    METHOD FOR SELECTING HUMAN INDUCED PLURIPOTENT STEM CELLS 有权
    选择人类诱导的大脑干细胞的方法

    公开(公告)号：US20130089870A1

    公开(公告)日：2013-04-11

    申请号：US13704584

    申请日：2011-06-15

    申请人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Mari Ohnuki

    发明人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Mari Ohnuki

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0081 ,  C12N5/0696 ,  C12N2501/15 ,  C12N2501/155

    摘要： The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.

    摘要翻译： 本发明提供了可以安全地用于移植的人诱导多能干细胞（iPS）细胞的选择方法。 也就是说，本发明提供了选择具有降低的分化阻力的人iPS细胞的方法，包括以下步骤：（1）诱导人iPS细胞的分化; （2）在步骤（1）之后检测剩余的未分化细胞; 和（3）选择在步骤（2）中检测到的剩余未分化细胞的速率等于或不超过对照细胞的人iPS细胞。

    INDUCED PLURIPOTENT STEM CELLS PRODUCED WITH OCT3/4, KLF AND SOX
    59.
    发明申请
    INDUCED PLURIPOTENT STEM CELLS PRODUCED WITH OCT3/4, KLF AND SOX 审中-公开
    诱导的OCT3 / 4，KLF和SOX生产的PLUIPOTENT干细胞

    公开(公告)号：US20130059386A1

    公开(公告)日：2013-03-07

    申请号：US13585729

    申请日：2012-08-14

    申请人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Keisuke Okita

    发明人： Shinya Yamanaka ,  Kazutoshi Takahashi ,  Keisuke Okita

    IPC分类号： C12N5/071 ,  C12N15/85

    CPC分类号： C07K14/4702 ,  C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2510/00 ,  C12N2740/15043

    摘要： The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.

    摘要翻译： 本发明涉及具有重新编程分化的体细胞以产生诱导多能干细胞（iPS）细胞的作用的核重编程因子。 本发明还涉及上述iPS细胞，产生和维持iPS细胞的方法，以及使用iPS细胞的方法，包括筛选和测试方法以及干细胞治疗方法。 本发明还涉及通过诱导上述iPS细胞的分化衍生的体细胞。