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    COMBINATIONS INCLUDING CRY3AA AND CRY6AA PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.)
    52.
    发明申请
    COMBINATIONS INCLUDING CRY3AA AND CRY6AA PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.) 有权
    包括CRY3AA和CRY6AA蛋白的组合，以防止CORN ROOTWORMS（DIABROTICA SPP。）中的抗性发展

    公开(公告)号：US20130263331A1

    公开(公告)日：2013-10-03

    申请号：US13643052

    申请日：2011-04-22

    申请人： Kenneth E. Narva ,  Thomas Meade ,  Kristin J. Fencil ,  Huarong Li ,  Timothy D. Hey ,  Aaron T. Woosley ,  Monica B. Olson

    发明人： Kenneth E. Narva ,  Thomas Meade ,  Kristin J. Fencil ,  Huarong Li ,  Timothy D. Hey ,  Aaron T. Woosley ,  Monica B. Olson

    IPC分类号： C12N15/82 ,  B32B1/02

    CPC分类号： C12N15/8286 ,  A01G22/00 ,  A01H5/10 ,  A01N37/18 ,  A01N43/50 ,  B32B1/02 ,  B65D85/00 ,  C07K14/325 ,  Y02A40/162 ,  Y10T428/13

    摘要： The subject invention relates in part to Cry3Aa in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry3Aa and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention. The subject invention also relates in part to combinations of Cry3Aa and Cry6Aa proteins “triple-stacked” or “multi-stacked” with another insecticidal protein(s) such as a Cry6Aa protein or binary Cry34/35 proteins. Thus, such embodiments target rootworms with three modes of action. Transgenic plants, including corn, comprising a cry6Aa gene and a cry3Aa gene are included within the scope of the subject invention.

    摘要翻译： 本发明部分地涉及Cry3Aa与Cry6Aa的组合。 本发明部分地涉及令人惊奇的发现，即Cry3Aa和Cry6Aa的组合可用于防止由玉米根虫（Diabrotica spp。）种群发展抗性（仅针对任一杀虫蛋白质系统）。 本发明中包括的是产生这些杀虫Cry蛋白质的植物，其可用于减轻玉米根虫群体可能发展的担忧，这些玉米根虫群可以单独抗这些杀虫蛋白系统。 产生这两种杀虫蛋白质系统的植物（和种植有这种植物的种植面包括在本发明的范围内）。 本发明还部分涉及Cry3Aa和Cry6Aa蛋白质“三叠层”或“多堆叠”与另一种杀虫蛋白如Cry6Aa蛋白或二元Cry34 / 35蛋白的组合。 因此，这样的实施例以三种作用模式来针对根虫。 包含cry6Aa基因和cry3Aa基因的包含玉米的转基因植物包括在本发明的范围内。

    Modified Cry35 proteins
    53.
    发明授权
    Modified Cry35 proteins 有权
    修饰的Cry35蛋白

    公开(公告)号：US07939651B2

    公开(公告)日：2011-05-10

    申请号：US12715744

    申请日：2010-03-02

    申请人： H. Ernest Schnepf ,  Kenneth E. Narva ,  Steven L. Evans

    发明人： H. Ernest Schnepf ,  Kenneth E. Narva ,  Steven L. Evans

    IPC分类号： C07H19/16 ,  C07K14/00 ,  C12N15/09

    CPC分类号： C07K14/325

    摘要： This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.

    摘要翻译： 本发明提供与野生型Cry35蛋白相比具有增强性质的改良的杀虫Cry35蛋白。 对这些蛋白质的修饰部分基于Cry35类中〜45kDa 149B1蛋白质和其他蛋白质的原子坐标和三维（3D）结构的分析。 本发明还包括编码这些修饰蛋白质的多核苷酸，以及产生这些修饰蛋白质的转基因植物。 本发明还提供了用这些修饰蛋白质控制植物害虫（包括根虫）的方法。 本发明的修饰蛋白包括涉及交换的片段，结构域和基序的嵌合毒素，如本文所讨论的。 本发明还提供了修饰Cry35蛋白的方法。

    Modified Cry35 proteins having altered protease stability
    54.
    发明授权
    Modified Cry35 proteins having altered protease stability 有权
    具有改变的蛋白酶稳定性的修饰的Cry35蛋白

    公开(公告)号：US07737263B2

    公开(公告)日：2010-06-15

    申请号：US11943814

    申请日：2007-11-21

    申请人： H. Ernest Schnepf ,  Kenneth E. Narva ,  Steven L. Evans

    发明人： H. Ernest Schnepf ,  Kenneth E. Narva ,  Steven L. Evans

    IPC分类号： C07H19/16 ,  C07K14/00 ,  C12N15/09

    CPC分类号： C07K14/325

    摘要： This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.

    摘要翻译： 本发明提供与野生型Cry35蛋白相比具有增强性质的改良的杀虫Cry35蛋白。 对这些蛋白质的修饰部分基于Cry35类中〜45kDa 149B1蛋白质和其他蛋白质的原子坐标和三维（3D）结构的分析。 本发明还包括编码这些修饰蛋白质的多核苷酸，以及产生这些修饰蛋白质的转基因植物。 本发明还提供了用这些修饰蛋白质控制植物害虫（包括根虫）的方法。 本发明的修饰蛋白包括涉及交换的片段，结构域和基序的嵌合毒素，如本文所讨论的。 本发明还提供了修饰Cry35蛋白的方法。

    Toxins active against pests
    56.
    发明授权
    Toxins active against pests 有权

    公开(公告)号：US06570005B1

    公开(公告)日：2003-05-27

    申请号：US09589477

    申请日：2000-06-07

    申请人： H. Ernest Schnepf ,  Carol Wicker ,  Kenneth E. Narva ,  Michele Walz ,  Brian A. Stockhoff

    发明人： H. Ernest Schnepf ,  Carol Wicker ,  Kenneth E. Narva ,  Michele Walz ,  Brian A. Stockhoff

    IPC分类号： C07H2104

    CPC分类号： C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  C12N2799/026 ,  Y02A40/162

    摘要： The subject invention concerns materials and methods useful in the control of non-mammalian pests and, particularly, plant pests. In a specific embodiment, the subject invention provides new Bacillus thuringiensis toxins useful for the control of lepidopterans. The subject invention further provides nucleotide sequences which encode the toxins of the subject invention. The nucleotide sequences of the subject invention can be used to transform hosts, such as plants, to express the pesticidal toxins of the subject invention. The subject invention further concerns novel nucleotide primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The primers are also useful as nucleotide probes to detect the toxin-encoding genes.

    Plant-optimized polynucleotides encoding approximately 15 kDa and approximately 45 kDa pesticidal proteins
    57.
    发明授权
    Plant-optimized polynucleotides encoding approximately 15 kDa and approximately 45 kDa pesticidal proteins 有权
    编码约15kDa和约45kDa杀虫蛋白的植物优化多核苷酸

    公开(公告)号：US06340593B1

    公开(公告)日：2002-01-22

    申请号：US09422487

    申请日：1999-10-21

    申请人： Guy A. Cardineau ,  Steven J. Stelman ,  Kenneth E. Narva

    发明人： Guy A. Cardineau ,  Steven J. Stelman ,  Kenneth E. Narva

    IPC分类号： C12N121

    CPC分类号： C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

    摘要： The subject invention provides new, plant-optimized polynucleotide sequences that encode pesticidal toxins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, for example, that make them particularly well-suited for optimized expression in plants. Using the polynucleotide sequences described herein, the transformation of plants can be accomplished, using techniques known to those skilled in the art, in order to confer pest resistance upon the plants. In preferred embodiments, the subject invention provides plant-optimized polynucleotide sequences which encode approximately 15 kDa and approximately 45 kDa pesticidal proteins.

    摘要翻译： 本发明提供了新的植物优化的编码杀虫毒素的多核苷酸序列。 与野生型序列相比，本发明的多核苷酸序列具有某些修饰，例如使得它们特别适合于在植物中优化表达。 使用本文所述的多核苷酸序列，可以使用本领域技术人员已知的技术来完成植物的转化，以便赋予植物上的害虫抗性。 在优选的实施方案中，本发明提供编码约15kDa和约45kDa杀虫蛋白的植物优化的多核苷酸序列。