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113 条记录 (耗时 0.027 秒)

    Nucleotide sequences which encode the pfk gene
    51.
    发明申请
    Nucleotide sequences which encode the pfk gene 审中-公开
    编码pfk基因的核苷酸序列

    公开(公告)号：US20070054379A1

    公开(公告)日：2007-03-08

    申请号：US10926293

    申请日：2004-08-26

    申请人： Bettina Mockel ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Walter Pfefferle

    IPC分类号： C12P13/08 ,  C12N9/10 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

    摘要： The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been Corynebacterium glutamicum, and a process for the production of an L-amino acid comprising culturing a coryneform bacteria comprising an overexpressed pfk gene, wherein said pfk gene comprises a polynucleotide having a nucleotide sequence which is at least 90% identical to the nucleotide of SEQ ID NO: 1 encoding a polypeptide having the enzymatic activity of a phosphofructokinase, accumulating said L-amino acid in the medium or in the cells of said bacterium, and isolated said L-amino acid.

    摘要翻译： 本发明涉及编码已经是谷氨酸棒杆菌的磷酸果糖激酶的核苷酸序列，以及L-氨基酸的生产方法，包括培养包含过表达的pfk基因的棒状细菌，其中所述pfk基因包含具有核苷酸的多核苷酸 序列，其与编码具有磷酸果糖激酶的酶活性的多肽的SEQ ID NO：1的核苷酸至少90％相同，在培养基中或所述细菌的细胞中积累所述L-氨基酸， 氨基酸。

    Nucleotide sequences coding for the citE gene
    52.
    发明授权
    Nucleotide sequences coding for the citE gene 失效
    编码citE基因的核苷酸序列

    公开(公告)号：US07129066B2

    公开(公告)日：2006-10-31

    申请号：US10375355

    申请日：2003-02-28

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Brigitte Bathe ,  Walter Pfefferle

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Brigitte Bathe ,  Walter Pfefferle

    IPC分类号： C12P13/08 ,  C12P13/04 ,  C12N9/88 ,  C07H21/04

    CPC分类号： C12Y401/03006 ,  C12N9/88

    摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide that is at least 70% identical with a polynucleotide that codes for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) polynucleotide that codes for a polypeptide containing an amino acid sequence that is at least 70% identical with the amino acid sequence of SEQ ID No. 2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and to a process for the production of L-amino acids by fermentation using coryneform bacteria in which at least the citE gene is present in attenuated form, and to the use of polynucleotides containing the sequences of the invention as hybridization probes.

    摘要翻译： 本发明涉及含有多核苷酸序列的多核苷酸序列，该多核苷酸序列选自a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 对于含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少含有 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及通过发酵使用其中至少有citE基因以减毒形式存在的棒状细菌产生L-氨基酸的方法， 使用含有本发明序列的多核苷酸作为杂交探针。

    Polynucleotide sequences which encode the zwa1 gene
    54.
    发明授权
    Polynucleotide sequences which encode the zwa1 gene 失效
    编码zwa1基因的多核苷酸序列

    公开(公告)号：US07074607B2

    公开(公告)日：2006-07-11

    申请号：US10640263

    申请日：2003-08-14

    申请人： Bettina Möckel ,  Walter Pfefferle ,  Achim Marx ,  Jörn Kalinowski ,  Brigitte Bathe ,  Alfred Pühler

    发明人： Bettina Möckel ,  Walter Pfefferle ,  Achim Marx ,  Jörn Kalinowski ,  Brigitte Bathe ,  Alfred Pühler

    IPC分类号： C12P1/00 ,  C12N1/20 ,  C12N15/00 ,  C07K1/00 ,  C07H21/02

    CPC分类号： C07K14/34 ,  C12N9/00 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码包含SEQ ID NO：2的氨基酸序列的多肽的至少70％的多核苷酸程度相同 b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％的程度相同的氨基酸序列）与a）和b的多核苷酸互补的多核苷酸 ）和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及在所用棒状细菌中扩增zwa1基因的L-氨基酸的发酵制剂的方法。

    Nucleotide sequences which code for the dep34 gene
    55.
    发明授权
    Nucleotide sequences which code for the dep34 gene 失效
    编码dep34基因的核苷酸序列

    公开(公告)号：US07029904B2

    公开(公告)日：2006-04-18

    申请号：US09946763

    申请日：2001-09-06

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Walter Pfefferle ,  Thomas Hermann ,  Brigitte Bathe

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Walter Pfefferle ,  Thomas Hermann ,  Brigitte Bathe

    IPC分类号： C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C07K14/34 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the dep34 efflux protein, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及具有编码dep34流出蛋白的多核苷酸序列的谷氨酸棒杆菌分离的多核苷酸，以及具有其中dep34基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带至少 根据SEQ ID NO：1的dep34基因，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Nucleotide sequences encoding the ptsH gene
    60.
    发明申请
    Nucleotide sequences encoding the ptsH gene 有权
    编码ptsH基因的核苷酸序列

    公开(公告)号：US20050009143A1

    公开(公告)日：2005-01-13

    申请号：US10916437

    申请日：2004-08-12

    申请人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    发明人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    IPC分类号： C12N1/21 ,  C12N9/12 ,  C12P13/04 ,  C12P13/08 ,  C07H21/04 ,  C12N1/20 ,  C12N9/10

    CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.

    摘要翻译： 本发明涉及含有多核苷酸序列的分离的多核苷酸序列，所述多核苷酸序列选自以下组：a）多核苷酸，其与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸具有至少70％的相同性。 2，b）编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及用于增加编码磷酸转移酶系统的组分H的ptsH基因的L-氨基酸的发酵生产的方法 ，以及使用上述多核苷酸作为引物或杂交探针。