公开(公告)号：US5626732A

公开(公告)日：1997-05-06

申请号：US561552

申请日：1995-11-21

申请人： Robert W. Allington

发明人： Robert W. Allington

IPC分类号： G01N27/447 ,  G01N27/26 ,  G01N27/443

CPC分类号： G01N27/44782 ,  G01N27/44721 ,  G01N27/44743

摘要： To detect bands in an electrophoresis capillary tube having a liquid separating medium, a light source and a light detector of a monitor are positioned on opposite sides of the capillary tube with the light source transmitting light through a first slit and light passing to the detector through a second slit after it has passed through the capillary tube. The first and second slits are aligned with the direction of motion of bands, have a maximum length of less than 500 micrometers and a maximum width of less than 200 micrometers. There are no vertical lengths in the capillary tube having a dimension greater than one third of its length between a sample injecting end and a detecting end of the capillary tube. A sample injector includes a vacuum chamber communicating with one end of the capillary tube to pull sample into the other end and a pressure sensor to measure the pressure in said vacuum chamber and generate a signal indicative thereof and integrate it to indicate the amount of sample pulled into the system.

摘要翻译： 为了检测具有液体分离介质的电泳毛细管中的条带，将监测器的光源和光检测器定位在毛细管的相对侧上，其中光源透过第一狭缝透射光并将光通过检测器通过 在其通过毛细管之后的第二狭缝。 第一和第二狭缝与带的运动方向对准，具有小于500微米的最大长度和小于200微米的最大宽度。 在毛细管中没有垂直长度，其长度大于样品注射端和毛细管的检测端之间的长度的三分之一。 样品注射器包括与毛细管的一端连通以将样品拉入另一端的真空室和用于测量所述真空室中的压力的​​压力传感器，并产生指示其的信号并将其整合以指示拉出的样品量 进入系统。

公开(公告)号：US4911837A

公开(公告)日：1990-03-27

申请号：US339473

申请日：1989-04-17

申请人： Robert W. Allington ,  Yoossef Tehrani ,  John N. Jones

发明人： Robert W. Allington ,  Yoossef Tehrani ,  John N. Jones

IPC分类号： B01D15/08 ,  B01D15/22 ,  G01N30/60 ,  G01N30/74

CPC分类号： B01D15/22 ,  G01N30/603 ,  G01N30/6017 ,  G01N30/6026 ,  G01N30/6052 ,  G01N30/74

摘要： To reduce tailing, the frit at the outlet to the liquid chromatographic column has a diameter substantially equal to that of the inside diameter of the outlet end of the column and less than two millimeters. It is held directly against the packing of the column within a cylindrical member having a shoulder with a central aperture at least the size of the diameter of the frit and cylindrical walls which are fastened to resist force in the direction of the axis of the column from its inlet to its outlet. A gasket seals the cylindrical holder against the column wall. The frit is formed of gold-plated nickel spheres sintered together at a temperature lower than the gold-nickel eutectic temperature of 950 degrees Celsius but above the phase-immiscibility temperature of 720 degrees Celsius. The size and shape of the frit is selected to reduce tailing by causing the flow path from the column to be uniform.

摘要翻译： 为了减少拖尾，液相色谱柱出口处的玻璃料的直径基本上等于柱的出口端的内径，直径小于2毫米。 它被直接抵靠在具有肩部的圆柱形构件的圆柱形构件中，所述圆柱形构件具有至少具有中心孔的至少大小的玻璃料和圆柱形壁的直径的尺寸，所述玻璃料和圆柱形壁被紧固以抵抗在柱的轴线方向上的力 其入口到其出口。 垫圈将柱形保持器密封在柱壁上。 玻璃料由在950℃以下的金 - 镍共晶温度下高于720℃的相互不混溶温度的温度下烧结在一起的镀金镍球形成。 选择玻璃料的尺寸和形状以通过使来自塔的流动路径均匀来减少拖尾。

公开(公告)号：US4869374A

公开(公告)日：1989-09-26

申请号：US99387

申请日：1987-09-21

申请人： Robert W. Allington

发明人： Robert W. Allington

IPC分类号： B01D15/08 ,  F04B49/06 ,  G01N30/32

CPC分类号： G01N30/32 ,  F04B49/065 ,  G01N2030/326

摘要： To provide smooth constant flow from a pump, a chromatographic system comprises: a chromatographic column having an inlet; a pump for supplying fluid to the inlet of the chromatographic column; a power means for the pump motor; positive and negative feedback loop means for controlling said power means; means for energizing and said positive and negative feedback control means; said negative feedback control means receiving a signal from said means for measuring flow rate and including means for comparing said signal with said corrected flow rate reference signal while said second feedback loop is energized to generate an error signal controlling said power means; and said positive feedback control means applying an acceleration voltage to said motor from a time a preset period after the initiation of a return stroke of said piston until after a timed duration.

公开(公告)号：US4781824A

公开(公告)日：1988-11-01

申请号：US902921

申请日：1986-09-02

申请人： Robert W. Allington

发明人： Robert W. Allington

IPC分类号： G01N30/32 ,  B01D15/08

CPC分类号： G01N30/32 ,  G01N2030/326

摘要： To stabilize flow rate in a single-stroke, syringe-type high pressure pump to a constant value after turn-on, a control system increases the pump speed until equilibrium conditions are reached and then pumps at the preset rate. In one embodiment, the control system senses the maximum rate of change of pressure, detects a pressure when the rate of change is two-thirds of the maximum, increases the pump rate until the pressure is three times that at the value of the detected pressure and begins pumping at the preset constant flow rate. In other embodiments: (1) a pumping system is operated at a flow rate ten times the preset flow rate and, when the time derivative of the pressure has dropped to nine tenths of its maximum, the pump motor speed is reduced to the preset flow rate; (2) the outflow of the column is measured under no load conditions at the desired flow rate and when a column is connected, the pumping speed is increased until the same outflow results before reducing the pumping rate; and (3) the system is operated in a constant pressure mode to a point at which the pressure is estimated to provide the desired flow rate, the flow rate measured, and if the flow rate is not the preset one, a new pressure measurement is set and the procedure repeated.

摘要翻译： 为了将单冲程注射器式高压泵的流量稳定在恒定值，启动后，控制系统会提高泵的转速，直到达到平衡条件，然后以预设的速率泵送。 在一个实施例中，控制系统感测压力的最大变化率，当变化率是最大值的三分之二时检测压力，增加泵速，直到压力是检测压力值的三倍 并以预设的恒定流速开始泵送。 在其他实施例中：（1）泵送系统以预设流量的十倍的流速操作，并且当压力的时间导数已经下降到其最大值的十分之一时，泵电机速度降低到预设流量 率; （2）在无负载条件下以期望的流速测量塔的流出量，并且当连接塔时，泵送速度增加直到在降低泵送速率之前产生相同的流出; 和（3）系统以恒定压力模式运行到估计压力的点以提供期望的流量，测量的流量，如果流量不是预设的，新的压力测量是 设置和重复的过程。

公开(公告)号：US4781464A

公开(公告)日：1988-11-01

申请号：US851233

申请日：1986-04-14

申请人： Robert W. Allington ,  Wylee D. Brunken ,  Lawrence L. Sedlak

发明人： Robert W. Allington ,  Wylee D. Brunken ,  Lawrence L. Sedlak

IPC分类号： G01N21/59 ,  G01N27/447 ,  G01N21/86

CPC分类号： G01N27/44721 ,  G01N21/5911

摘要： To scan gels optically, a gel scanner includes a gel holder and an optical system mounted for motion with respect to each other. The optical system includes a tungsten halogen lamp, first aperture, second aperture, beam splitter and first and second light paths; the first aperture having a dimension in the direction of motion of the gel with respect to the optical system of less than 0.5 millimeter and a dimension in a direction transverse to the direction of motion of less than 10 millimeters. The lamp and first aperture are mounted on one side of said gel holder and the second aperture is mounted on the other side of said gel holder. The beam splitter transmits light in a first direction along the first light path and in a second direction along a second light path with the: (1) first light path including a first photodetector and an interference filter for passing light within the range of 400 nanometers wavelength to 700 nanometers wavelength to it; and (2) the second light path having a second photodetector.

摘要翻译： 为了光学扫描凝胶，凝胶扫描仪包括凝胶保持器和安装成相对于彼此运动的光学系统。 光学系统包括卤钨灯，第一光圈，第二光圈，光束分离器和第一和第二光路; 所述第一孔具有在所述凝胶相对于所述光学系统的运动方向上的尺寸小于0.5毫米，并且在垂直于所述运动方向的方向上的尺寸小于10毫米。 灯和第一孔安装在所述凝胶保持器的一侧上，第二孔安装在所述凝胶保持器的另一侧上。 分束器沿着第一光路沿着第一方向透射光，沿着第二光路在第二方向上透射光，其中：（1）第一光路包括第一光电检测器和用于使光在400纳米范围内通过的干涉滤光器 波长到700纳米波长; 和（2）第二光路具有第二光电检测器。

公开(公告)号：US4775481A

公开(公告)日：1988-10-04

申请号：US83889

申请日：1987-08-10

申请人： Robert W. Allington

发明人： Robert W. Allington

IPC分类号： G01N30/32 ,  B01D15/08

CPC分类号： G01N30/32 ,  G01N2030/326

摘要： To stabilize flow rate in a single-stroke, syringe-type high pressure pump at a constant value after turn-on, a control system increases the pump speed until equilibrium conditions are reached and then pumps at the present rate. In one embodiment, the system is operated in a constant pressure mode under the control of a feedback system to a point at which the pressure is estimated to provide the desired flow rate, with feedback being provided from a pressure measurement and from a flow rate measurement. The feedback from the flow measurement is increased logarithmically with the rate of pumping prior to being compared with the pressure error signal and the comparison between the previous error signal and the logarithmic flow rate signal is used to control the speed of pumping.

摘要翻译： 为了稳定单次冲程注射器式高压泵的流量，启动后的恒定值，控制系统会提高泵的速度，直到达到平衡条件，然后以当前的速度泵送。 在一个实施例中，系统在反馈系统的控制下在恒定压力模式下操作到估计压力以提供所需流量的点，反馈由压力测量和流速测量提供 。 来自流量测量的反馈在与压力误差信号进行比较之前与泵送速率对数地增加，并且使用先前的误差信号和对数流量信号之间的比较来控制泵送速度。

公开(公告)号：US4769153A

公开(公告)日：1988-09-06

申请号：US902974

申请日：1986-09-02

申请人： Robert W. Allington

发明人： Robert W. Allington

IPC分类号： G01N30/32 ,  B01D15/08

CPC分类号： G01N30/32 ,  G01N2030/326

摘要： To stabilize flow rate in a single-stroke, syringe-type high pressure pump to a constant value after turn-on, a control system increases the pump speed until equilibrium conditions are reached and then pumps at the preset rate. The pumping system is operated at a flow rate ten times the preset flow rate. The control system senses the maximum rate of change of pressure, detects when the rate of change has dropped to nine-tenths of the maximum and, when the time derivative of the pressure has dropped to nine tenths of its maximum, the pump motor speed is reduced to the preset flow rate.

摘要翻译： 为了将单冲程注射器式高压泵的流量稳定在恒定值，启动后，控制系统会提高泵的转速，直到达到平衡条件，然后以预设的速率泵送。 泵送系统以预设流量的十倍的流量运行。 控制系统检测最大压力变化率，检测变化率何时下降到最大值的九分之一，当压力的时间导数下降到其最大值的十分之一时，泵电机转速为 降低到预设流量。

公开(公告)号：US4765890A

公开(公告)日：1988-08-23

申请号：US104437

申请日：1987-10-05

申请人： Abolghassem Y. Tehrani ,  John N. Jones ,  Robert W. Allington

发明人： Abolghassem Y. Tehrani ,  John N. Jones ,  Robert W. Allington

IPC分类号： B01D15/08 ,  G01N30/60 ,  G01N30/74

CPC分类号： B01D15/08 ,  G01N30/603 ,  G01N30/6017 ,  G01N30/6026 ,  G01N30/6052 ,  G01N30/74

摘要： To reduce tailing, the frit at the outlet to the liquid chromatographic column has a diameter substantially equal to that of the inside diameter of the outlet end of the column and less than two millimeters. It is held directly against the packing of the column within a cylindrical member having a shoulder with a central aperture at least the size of the diameter of the frit and cylindrical walls which are fastened to resist force in the direction of the axis of the column from its inlet to its outlet. A gasket seals the cylindrical holder against the column wall. The frit is formed of gold-plated nickel spheres sintered together at a temperature lower than the gold-nickel eutectic temperature of 950 degrees Celsius but above the phase-immiscibility temperature of 720 degrees Celsius. The size and shape of the frit is selected to reduce tailing by causing the flow path from the column to be uniform.

公开(公告)号：US4673334A

公开(公告)日：1987-06-16

申请号：US825690

申请日：1986-02-03

申请人： Robert W. Allington ,  John D. Hull

发明人： Robert W. Allington ,  John D. Hull

IPC分类号： F04B43/12

CPC分类号： F04B43/1292

摘要： To increase precision of flow in multiple channels of a peristaltic pump, a plurality of spring biased cassettes are mounted to cooperate with a spool having a plurality of elongated rollers which orbit about its center so that each elongated roller in succession compresses a plurality of flexible tubes in the channels against corresponding cam surfaces in the cassettes, spaced from the rollers by surfaces of the cassettes which are in contact with the rolles. In each cassette, the cam is mounted between side plates against which the rollers roll, which are spring biased against the rollers and hinged at a center location to provide precision determined only by the precision of the cam to side plate edge dimension. The cam is shaped to reduce pulses during roller lift-off from the cam surface to provide for pulse-free flow.

摘要翻译： 为了提高蠕动泵的多个通道中的流动精度，安装了多个弹簧偏置的盒，以与具有多个细长辊的卷轴配合，所述卷轴围绕其中心轨道运动，使得每个细长辊相继地压缩多个柔性管 在与盒中的相应凸轮表面相对的通道中，与辊的表面间隔开与辊相接触的盒。 在每个盒中，凸轮安装在侧板之间，辊子滚动抵靠辊子弹簧偏压，并且在中心位置处铰接，以提供仅由凸轮到侧板边缘尺寸的精度确定的精度。 凸轮被成形为在辊子从凸轮表面移除时减少脉冲以提供无脉冲的流动。

公开(公告)号：US4536091A

公开(公告)日：1985-08-20

申请号：US44763

申请日：1979-06-01

申请人： Robert W. Allington

发明人： Robert W. Allington

IPC分类号： G01N21/59 ,  G01N21/01 ,  G01N21/25

CPC分类号： G01N21/255

摘要： To reduce noise in an absorbance monitor in which the light source is a feedback controlled deuterium lamp, a mirror focuses light from a part of the central bright spot in the deuterium lamp through a small aperture in an aperture plate which blocks all other light passing through the optical detecting and electrical sensor station. The light passing through the aperture is split into two unpolarized beams within the optical detecting and electrical sensor station, one of which is transmitted through an aperture optically at least as large as the image of the aperture near the lamp and into a flow cell to sense the absorbance or transmittance of an effluent. After the sensing beam has passed through the effluent, it is electrically compared with the other beam which has been passed through an aperture to cancel out the common-mode noise and in some applications the absorbance or transmittance which may be due to the solvent or other carrier of the sample.

摘要翻译： 为了减少光源是反馈控制的氘灯的吸光度监测器中的噪声，镜子将来自氘灯中的中心亮点的一部分的光聚焦在孔板中的小孔，该小孔阻挡所有其他光通过 光学检测和电传感器站。 通过孔径的光被分成光学检测和电传感器站内的两个非偏振光束，其中之一通过光学透射的孔径至少与灯附近的孔径的图像一样大，并且进入流动池以感测 流出物的吸光度或透光率。 在感测光束通过流出物之后，它与已经通过孔径的另一个光束进行电气比较，以抵消共模噪声，并且在一些应用中，可能由于溶剂或其它原因引起的吸光度或透射率 载体的样品。