公开(公告)号：US20080251715A1

公开(公告)日：2008-10-16

申请号：US12056630

申请日：2008-03-27

申请人： Evgenij Nikolaev ,  Jochen Franzen

发明人： Evgenij Nikolaev ,  Jochen Franzen

IPC分类号： H01J49/26

CPC分类号： G21K1/093 ,  H01J49/065 ,  H01J49/38

摘要： In a mass spectrometer that uses a space-restricted magnetic field, such as an ion cyclotron resonance mass spectrometer, ions with a wide mass range generated in an ion supply located outside the magnetic field are transported in the direction of the magnetic field lines to an ion storage device located inside the magnetic field without losing ions by guiding the ions through the region in which the magnetic field strength increases with a special ion guide. This ion guide consists of an arrangement of coaxial ring diaphragms which are alternately supplied with the phases of an RF voltage. In an alternative embodiment, the ion guide uses two wires wound in a double helix where each wire is supplied with one phase of a two-phase RF voltage.

摘要翻译： 在使用诸如离子回旋共振质谱仪的空间受限的磁场的质谱仪中，在位于磁场外部的离子供体中产生的质量范围宽的离子在磁场线的方向上被传送到 离子存储装置，其位于磁场内部，而不会通过引导离子通过特殊离子导向器的磁场强度增加的区域而损失离子。 该离子导向器由交替地提供有RF电压的相位的同轴环形隔膜的布置组成。 在替代实施例中，离子导向器使用缠绕在双螺旋线中的两条线，其中每条线被提供有两相RF电压的一相。

公开(公告)号：US07368711B2

公开(公告)日：2008-05-06

申请号：US11197129

申请日：2005-08-04

申请人： Jochen Franzen ,  Evgenij Nikolaev

发明人： Jochen Franzen ,  Evgenij Nikolaev

IPC分类号： B01D59/44

CPC分类号： H01J49/38

摘要： The invention relates to a measuring cell for an ion cyclotron resonance mass spectrometer (FTMS). The invention provides a measuring cell which, on the one hand, consists of two ion-repelling RF grids at the front ends as trapping electrodes and thus produces a pure cyclotron motion of the ions without the usually co-existing magnetron motion and, on the other hand, measures a multiplied cyclotron frequency by means of a plurality of detection electrodes, whereby either a higher mass accuracy or a shorter measuring time can be achieved.

摘要翻译： 本发明涉及一种用于离子回旋共振质谱仪（FTMS）的测量池。 本发明提供了一种测量电池，其一方面由前端的两个离子排斥RF栅格组成，作为捕获电极，从而产生离子的纯回旋加速器，而不需要通常共存的磁控管运动，并且在 另一方面，通过多个检测电极测量相乘的回旋加速器频率，由此可以实现更高的质量精度或更短的测​​量时间。

公开(公告)号：US07301145B2

公开(公告)日：2007-11-27

申请号：US11229047

申请日：2005-09-16

申请人： Armin Holle ,  Michael Schubert

发明人： Armin Holle ,  Michael Schubert

IPC分类号： H01J49/44

CPC分类号： H01J49/0031 ,  H01J49/0045 ,  H01J49/403 ,  H01J49/4215

摘要： The invention relates to methods and devices for measuring daughter ion spectra (also called fragment ion spectra or MS/MS spectra) in time-of-flight mass spectrometers with orthogonal injection of the ions. The invention filters the parent ions selected to be fragmented by a mass filter before they are injected into the time-of-flight mass spectrometer, fragments the selected ions in a first stage of the time-of-flight mass spectrometer within a collision cell filled with collision gas at collision energies between one and five kiloelectron-volts, further accelerates the fragment ions and measures the fragment ions in a second stage of the time-of-flight mass spectrometer.

摘要翻译： 本发明涉及通过正交注射离子来测量飞行时间质谱仪中的子离子谱（也称为碎片离子谱或MS / MS谱）的方法和装置。 本发明在被注入飞行时间质谱仪之前过滤选择被质量过滤器分段的亲本离子，在飞行时间质谱仪的第一阶段将所选择的离子碎片在填充的碰撞池内 碰撞气体在一个和五千伏电压之间的碰撞能量下，进一步加速碎片离子并在飞行时间质谱仪的第二阶段测量碎片离子。

公开(公告)号：US07235781B2

公开(公告)日：2007-06-26

申请号：US11226855

申请日：2005-09-14

申请人： Andreas Haase ,  Markus Kayser ,  Jens Höhndorf ,  Armin Holle

发明人： Andreas Haase ,  Markus Kayser ,  Jens Höhndorf ,  Armin Holle

IPC分类号： H01J49/00

CPC分类号： H01J49/164 ,  H01S3/005 ,  H01S3/08022

摘要： The invention relates to a laser system for the ionization of a sample by matrix-assisted laser desorption in mass spectrometric analysis. The invention consists in providing a laser system which generates a spatially modulated intensity distribution with intensity spots on the sample, which significantly improves the quality and the robustness of the mass spectrometric analysis compared with a spatially homogeneous intensity distribution and, in particular, improves the ionization efficiency and, for a time-of-flight mass spectrometer with axial injection, the mass resolution and the signal-to-noise ratio.

摘要翻译： 本发明涉及一种用于在质谱分析中通过基质辅助激光解吸电离样品的激光系统。 本发明提供一种激光系统，其产生具有样品上强度斑点的空间调制强度分布，与空间均匀的强度分布相比，显着提高了质谱分析的质量和鲁棒性，并且特别地提高了电离 效率，对于具有轴向注射的飞行时间质谱仪，质量分辨率和信噪比。

公开(公告)号：US20070114438A1

公开(公告)日：2007-05-24

申请号：US11559086

申请日：2006-11-13

申请人： Armin Holle ,  Gregor Przybyla

发明人： Armin Holle ,  Gregor Przybyla

IPC分类号： H01J27/00

CPC分类号： H01J49/10 ,  H01J37/32862

摘要： The invention relates to the automatic cleaning of ion sources inside mass spectrometers, especially the cleaning of ion sources where the ions are generated by matrix-assisted laser desorption (MALDI). The invention consists in cleaning the electrodes of the ion source, which are contaminated with organic material, in the mass spectrometer itself by etching with reactive ions produced by an electrically generated gas discharge in a specially admitted reactant gas.

摘要翻译： 本发明涉及质谱仪内离子源的自动清洗，特别是通过基质辅助激光解吸（MALDI）产生离子的离子源的清洗。 本发明通过在特别允许的反应气体中通过用电气产生的气体放电产生的活性离子进行蚀刻来清洁质谱仪本身中被有机材料污染的离子源的电极。

公开(公告)号：US20070068817A1

公开(公告)日：2007-03-29

申请号：US11534252

申请日：2006-09-22

申请人： Evgenij Nikolaev ,  Jochen Franzen

发明人： Evgenij Nikolaev ,  Jochen Franzen

IPC分类号： G01N27/00

CPC分类号： G01N27/447

摘要： Components of a mixture are separated by feeding charged molecules of the components into a end surface of a suitable medium, for example a gel. The molecules are drawn in a first direction through the medium by means of an DC electric field, while at the same time being subjected to an alternating voltage with a strongly asymmetric profile in a direction transverse to the first direction. The nonlinear behavior of the electrically-generated migration causes a large number of molecules to migrate transversely out of the medium while only a small number of molecules reach the opposite end surface of the medium. A superimposed DC voltage in the transverse direction selects which of the mixture components migrate all the way through the medium in the first direction. The separated components can be sampled from the opposite end surface and from points on the upper and lower medium surfaces.

摘要翻译： 通过将组分的带电分子送入合适的介质例如凝胶的端表面来分离混合物的组分。 分子通过DC电场沿着第一方向通过介质被拉伸，同时在横向于第一方向的方向上经受具有强非对称轮廓的交流电压。 电生成迁移的非线性行为导致大量分子横向移出介质，而只有少量分子到达介质的相对端面。 横向上的叠加的直流电压选择哪个混合组分在第一方向上一直迁移通过介质。 分离的组件可以从相对端表面和上介质和下介质表面上的点进行采样。

公开(公告)号：US07193223B2

公开(公告)日：2007-03-20

申请号：US11003017

申请日：2004-12-02

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： H01J27/00 ,  H01J49/10

CPC分类号： H01J49/165 ,  G01N30/7266 ,  H01J49/0463

摘要： The invention relates to the ionization of analyte molecules on a solid surface close to atmospheric pressure as an ion source for mass spectrometers. The invention uses a spray mist from an electrospray apparatus to ionize the analyte molecules, for example a spray mist created by spraying pure water, which generates predominantly multiply charged ions of the analyte molecules which are particularly suitable for fragmentation.

摘要翻译： 本发明涉及作为质谱仪离子源的分析物分子在接近大气压的固体表面上的离子化。 本发明使用来自电喷雾装置的喷雾来离子化分析物分子，例如通过喷射纯水产生的喷雾，其主要产生特别适合于碎裂的分析物分子的多电荷离子。

公开(公告)号：US20060186332A1

公开(公告)日：2006-08-24

申请号：US11351350

申请日：2006-02-09

申请人： Andreas Haase ,  Markus Kayser ,  Jens Hohndorf ,  Armin Holle

发明人： Andreas Haase ,  Markus Kayser ,  Jens Hohndorf ,  Armin Holle

IPC分类号： H01J49/00

CPC分类号： H01J49/164 ,  H01J49/067

摘要： The invention relates to a laser system for the ionization of a sample by matrix-assisted laser desorption in mass spectrometric analysis. The invention consists in providing an adjustable laser system which, in one setting, generates a single intensity peak on the sample and, in another setting, a multiplicity of intensity peaks, with the half-width, intensity, spatial arrangement and/or degree of spatial modulation of the single intensity peak and/or the intensity peaks being adjustable.

摘要翻译： 本发明涉及一种用于在质谱分析中通过基质辅助激光解吸电离样品的激光系统。 本发明提供了一种可调激光系统，其在一个设置中在样品上产生单个强度峰值，并且在另一设置中产生多个强度峰值，其具有半宽度，强度，空间排列和/或程度 单个强度峰值和/或强度峰值的空间调制是可调节的。

公开(公告)号：US20060186331A1

公开(公告)日：2006-08-24

申请号：US11343235

申请日：2006-01-30

申请人： Ralf Hartmer ,  Andreas Brekenfeld

发明人： Ralf Hartmer ,  Andreas Brekenfeld

IPC分类号： H01J49/00

CPC分类号： H01J49/424 ,  H01J49/0072

摘要： The invention relates to a method and instrument for the fragmentation of large molecular analyte ions, preferably biopolymer ions, by reactions between multiply charged positive analyte ions and negative reactant ions in RF quadrupole ion traps. Some of these reactions involve electron transfer reactions with subsequent dissociation of the biopolymer analyte ions, and some involve the loss of a proton, leading to stable product ions. The invention can use any type of ion traps, particularly three-dimensional RF quadrupole ion traps, for the reactions between positive and negative ions. The fragmentation yield can be increased because ions that remain stable as radical cations after transfer of an electron are further fragmented by collisionally induced fragmentation, forming fragment ions that are typical of electron transfer, and not those typical of collisionally induced fragmentation. The invention preferentially introduces positive ions and negative ions into the ion trap sequentially through the same aperture.

摘要翻译： 本发明涉及通过RF四极离子阱中多重带电的正离子分析物和负反应物离子之间的反应来破碎大分子分子离子，优选生物聚合物离子的方法和仪器。 这些反应中的一些涉及电子转移反应，随后生物聚合物分析物离子的解离，一些涉及质子的损失，导致稳定的产物离子。 本发明可以使用任何类型的离子阱，特别是三维RF四极离子阱，用于正离子和负离子之间的反应。 碎片产率可以增加，因为在电子转移后作为自由基阳离子保持稳定的离子通过碰撞诱导的断裂进一步破碎，形成典型的电子转移的碎片离子，而不是典型的碰撞诱导的分裂。 本发明优选地将正离子和负离子依次通过相同的孔引入离子阱。

公开(公告)号：US20060027743A1

公开(公告)日：2006-02-09

申请号：US11197129

申请日：2005-08-04

申请人： Jochen Franzen ,  Evgenij Nikolaev

发明人： Jochen Franzen ,  Evgenij Nikolaev

IPC分类号： H01J49/40 ,  B01D59/44

CPC分类号： H01J49/38

摘要： The invention relates to a measuring cell for an ion cyclotron resonance mass spectrometer (FTMS). The invention provides a measuring cell which, on the one hand, consists of two ion-repelling RF grids at the front ends as trapping electrodes and thus produces a pure cyclotron motion of the ions without the usually co-existing magnetron motion and, on the other hand, measures a multiplied cyclotron frequency by means of a plurality of detection electrodes, whereby either a higher mass accuracy or a shorter measuring time can be achieved.

摘要翻译： 本发明涉及一种用于离子回旋共振质谱仪（FTMS）的测量池。 本发明提供了一种测量电池，其一方面由前端的两个离子排斥RF栅格组成，作为捕获电极，从而产生离子的纯回旋加速器，而不需要通常共存的磁控管运动，并且在 另一方面，通过多个检测电极测量相乘的回旋加速器频率，由此可以实现更高的质量精度或更短的测​​量时间。