公开(公告)号：US11754572B2

公开(公告)日：2023-09-12

申请号：US13102212

申请日：2011-05-06

Applicant: Markus Kostrzewa ,  Jochen Franzen

Inventor： Markus Kostrzewa ,  Jochen Franzen

IPC: C12Q1/04 ,  G01N33/68 ,  G01N33/569

CPC classification number: G01N33/6851 ,  G01N33/569

Abstract: A method of detecting specified target microbes in different types of sample uses only one to two cultivation steps for the enrichment of the target microbes from the sample, preferably in selective culture media, combined with a mass spectrometric detection method that identifies the target microbes in mixtures with other microbes even if the target microbes account for only a small proportion of the mixture. The sample may be a food sample, a sample from bodies of water used for bathing, a soil sample, a swabbed sample, a stool sample, an impactor sample with collected aerosol particles, amongst many others. The detection method is several days faster than standard methods and less expensive.

公开(公告)号：US08384019B2

公开(公告)日：2013-02-26

申请号：US12767273

申请日：2010-04-26

Applicant: Claus Köster ,  Jochen Franzen

Inventor： Claus Köster ,  Jochen Franzen

IPC: B01D59/44

CPC classification number: H01J49/02 ,  H01J49/26 ,  H01J49/425

Abstract: In a Kingdon ion trap in which harmonic ion oscillation in a potential well in a longitudinal direction is completely decoupled from ion oscillation in a direction transverse to the longitudinal direction, ions enter the trap via an entrance tube extending through, but electrically insulated from, one of the Kingdon trap housing electrodes and located outside the minimum of the potential well in the longitudinal direction. The geometry of the Kingdon trap is arranged so that the oscillating ions introduced through the entrance tube cannot return to the entrance tube until they have performed several longitudinal oscillations during which time heavy ions can be introduced into the trap.

Abstract translation: 在Kingdon离子阱中，纵向方向的势阱中的谐波离子振荡在垂直于纵向的方向上与离子振荡完全解耦，离子通过入口管进入陷阱，该入口管延伸通过一个 的Kingdon陷阱壳体电极并且位于纵向方向上的势阱的最小值之外。 Kingdon陷阱的几何形状被布置成使得通过入口管引入的振荡离子不能返回到入口管，直到它们执行几个纵向振荡，在该时间期间可以将重离子引入陷阱。

公开(公告)号：US08319180B2

公开(公告)日：2012-11-27

申请号：US13208803

申请日：2011-08-12

Applicant: Evgenij Nikolaev ,  Jochen Franzen

Inventor： Evgenij Nikolaev ,  Jochen Franzen

IPC: H01J49/28 ,  H01J49/26 ,  H01J49/42

CPC classification number: H01J49/4245

Abstract: The invention relates to measuring devices of an electrostatic Fourier transform mass spectrometer and measurement methods for the acquisition of mass spectra with high mass resolution. The measuring device includes electrostatic measuring cells according to the Kingdon principle, in which ions can, when appropriate voltages are applied, orbit on circular trajectories around the cylinder axis between two concentric cylindrical surfaces, which are composed of specially shaped sheath electrodes, insulated from each other by parabolic gaps, and can harmonically oscillate in the axial direction, independently of their orbiting motion. In the longitudinal direction, the two cylindrical surfaces of the measuring cell are divided by the parabolic separating gaps into different types of double-angled and tetragonal sheath electrode segments. Appropriate voltages at the sheath electrode segments generate a potential distribution between the two concentric cylindrical surfaces which forms a parabolic potential well in the axial direction for orbiting ions. The ion clouds oscillating harmonically in the axial direction in this potential well induce image currents in suitable electrodes, from which the oscillation frequencies can be determined by Fourier analyses.

Abstract translation: 本发明涉及静电傅立叶变换质谱仪的测量装置和用于以高质量分辨率采集质谱的测量方法。 测量装置包括根据Kingdon原理的静电测量单元，其中当适当的电压施加时，离子可以在围绕圆柱体轴线的圆周轨迹上轨道在两个同心圆柱表面之间，这两个同心圆柱表面由特殊形状的护套电极组成 另一个是抛物线间隙，并且可以在轴向方向上谐振振荡，而不受其轨道运动的影响。 在纵向方向上，测量单元的两个圆柱形表面被抛物线分离间隔分成不同类型的双角和四角形电极段。 护套电极段处的适当电压在两个同心圆柱形表面之间产生电位分布，这两个同心圆柱形表面在轴向方向上很好地形成抛物线势垒，用于轨道离子。 在该势阱中在轴向上谐波振荡的离子云在合适的电极中引起图像电流，通过傅立叶分析可以确定振荡频率。

公开(公告)号：US20120264162A1

公开(公告)日：2012-10-18

申请号：US13523167

申请日：2012-06-14

Applicant: Vadim Markovich Govorun ,  Jochen Franzen

Inventor： Vadim Markovich Govorun ,  Jochen Franzen

IPC: G01N27/62

CPC classification number: G01N33/6851 ,  C12Q1/18

Abstract: Microorganisms, particularly bacteria, are identified and characterized on the basis of a mass spectrometric measurement of their protein profiles with ionization by matrix-assisted laser desorption. In order to measure the microbial resistance to antibiotics, the protein profiles of microorganisms are measured after cultivation for a short time duration in nutrient media containing the antibiotics.

Abstract translation: 基于通过基质辅助激光解吸电离的蛋白质谱的质谱测定，鉴定和表征微生物，特别是细菌。 为了测量微生物对抗生素的抗性，微生物的蛋白质分布在含有抗生素的营养培养基中培养短时间后测量。

公开(公告)号：US20120228492A1

公开(公告)日：2012-09-13

申请号：US13503202

申请日：2010-11-15

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: H01J49/26

CPC classification number: H01J49/421 ,  H01J49/401

Abstract: The invention relates to ions guided by gas flows in mass spectrometers, particularly in RF multipole systems, and to RF quadrupole mass filters and their operation with gas flows in tandem mass spectrometers. The invention provides a tandem mass spectrometer in which the RF quadrupole mass filter is operated at vacuum pressures in the medium vacuum pressure regime, utilizing a gas flow to drive the ions are through the mass filter. Vacuum pressures between 0.5 to 10 pascal are maintained in the mass filter. The mass filter may be enclosed by a narrow enclosure to guide the gas flow. The quadrupole mass filter may be followed by an RF multipole system, operated at the same vacuum pressure, serving as fragmentation cell to fragment the selected parent ions. The fragmentation cell may be enclosed by the same enclosure which already encloses the mass filter, so the ions may be driven by the same gas flow at the same vacuum pressure, greatly simplifying the required vacuum pumping system in tandem mass spectrometers. There are many other applications utilizing gas flows including supersonic gas jets in mass spectrometry.

Abstract translation: 本发明涉及在质谱仪，特别是RF多极系统中的气流引导的离子，以及RF串联质谱仪和其在串联质谱仪中的气流的操作。 本发明提供一种串联质谱仪，其中RF四极杆质量过滤器在中等真空压力状态下在真空压力下操作，利用气流驱动离子通过质量过滤器。 在质量过滤器中保持0.5至10帕斯卡之间的真空压力。 质量过滤器可以被狭窄的外壳包围以引导气流。 四极质量过滤器之后可以是在相同的真空压力下操作的RF多极体系统，用作碎裂细胞以分离所选择的母体离子。 分裂单元可以被已经包围质量过滤器的相同外壳封闭，因此离子可以在相同的真空压力下由相同的气流驱动，大大简化了串联质谱仪中所需的真空泵送系统。 在质谱法中还有许多其他的应用，包括使用超声波气体射流的气流。

公开(公告)号：US20120043461A1

公开(公告)日：2012-02-23

申请号：US13208803

申请日：2011-08-12

Applicant: Evgenij Nikolaev ,  Jochen Franzen

Inventor： Evgenij Nikolaev ,  Jochen Franzen

IPC: H01J49/28

CPC classification number: H01J49/4245

Abstract: The invention relates to measuring devices of an electrostatic Fourier transform mass spectrometer and measurement methods for the acquisition of mass spectra with high mass resolution. The measuring device includes electrostatic measuring cells according to the Kingdon principle, in which ions can, when appropriate voltages are applied, orbit on circular trajectories around the cylinder axis between two concentric cylindrical surfaces, which are composed of specially shaped sheath electrodes, insulated from each other by parabolic gaps, and can harmonically oscillate in the axial direction, independently of their orbiting motion. In the longitudinal direction, the two cylindrical surfaces of the measuring cell are divided by the parabolic separating gaps into different types of double-angled and tetragonal sheath electrode segments. Appropriate voltages at the sheath electrode segments generate a potential distribution between the two concentric cylindrical surfaces which forms a parabolic potential well in the axial direction for orbiting ions. The ion clouds oscillating harmonically in the axial direction in this potential well induce image currents in suitable electrodes, from which the oscillation frequencies can be determined by Fourier analyses.

Abstract translation: 本发明涉及静电傅立叶变换质谱仪的测量装置和用于以高质量分辨率采集质谱的测量方法。 测量装置包括根据Kingdon原理的静电测量单元，其中当适当的电压施加时，离子可以在围绕圆柱体轴线的圆周轨迹上轨道在两个同心圆柱形表面之间，这两个同心圆柱表面由特殊形状的护套电极组成 另一个是抛物线间隙，并且可以在轴向方向上谐振振荡，而不受其轨道运动的影响。 在纵向方向上，测量单元的两个圆柱形表面被抛物线分离间隔分成不同类型的双角和四角形电极段。 护套电极段处的适当电压在两个同心圆柱形表面之间产生电位分布，这两个同心圆柱形表面在轴向方向上很好地形成抛物线势垒，用于轨道离子。 在该势阱中在轴向上谐波振荡的离子云在合适的电极中引起图像电流，通过傅立叶分析可以确定振荡频率。

公开(公告)号：US20110272573A1

公开(公告)日：2011-11-10

申请号：US13103672

申请日：2011-05-09

Applicant: Markus Kostrzewa ,  Jochen Franzen

Inventor： Markus Kostrzewa ,  Jochen Franzen

IPC: H01J49/40

CPC classification number: H01J49/0027 ,  H01J49/164 ,  H01J49/40

Abstract: The invention relates to acquisition techniques for time-of-flight mass spectra with ionization of the analyte substances by matrix assisted laser desorption. Generally speaking, these acquisition techniques involve adding together a large number of individual time-of-flight spectra, each with restricted dynamic measuring range, to form a sum spectrum. The invention provides a method that improves, in particular, the reproducibility, the concentration accuracy and therefore the ability to quantify the mass spectra. Particular embodiments also increase the dynamic range of measurement. For this purpose, multiple series of mass spectra are acquired, whereby the energy density in the laser spot is increased in discrete steps. As a result, many ion signals saturate the detector and can therefore no longer be evaluated. However, it is possible to employ a technique in which the ion beam is increasingly defocused, or, secondly, to replace parts of the spectrum that are subject to saturation by intensity extrapolations from mass spectra acquired with lower energy density. In the first case, hundreds or thousands of individual mass spectra must be added together in order to increase the dynamic measuring range. In the second case, the finally acquired mass spectrum, with its replacements, forms a mass spectrum with a high dynamic measuring range, improved reproducibility and better concentration accuracy. The gradient of the increasing intensities of the ion signals, as a function of the energy density, supplies additional information about the proton affinity of the analyte ions. The concentration accuracy is enhanced because the increase in the number of proton donors in the ionization plasma leads to an increase in the ionization of those analyte substances that have a lower proton affinity.

Abstract translation: 本发明涉及通过基质辅助激光解吸附物质分析物质离子化的飞行时间质谱采集技术。 一般来说，这些采集技术包括将大量具有限制动态测量范围的单个飞行时间频谱相加在一起以形成和频谱。 本发明提供了一种特别改进重现性，浓度精度以及因此量化质谱的能力的方法。 具体实施例还增加了测量的动态范围。 为此，获得了多个质谱系列，从而在离散步骤中激光斑点的能量密度增加。 因此，许多离子信号使检测器饱和，因此不再能被评估。 然而，可以采用这样一种技术，其中离子束越来越散焦，或者其次，通过由具有较低能量密度获得的质谱的强度外推替代经受饱和的部分光谱。 在第一种情况下，为了增加动态测量范围，必须将数百或数千个单个质谱加在一起。 在第二种情况下，最终获得的质谱与其替代物形成具有高动态测量范围，改进的再现性和更好的浓度精度的质谱。 作为能量密度的函数的离子信号的增加强度的梯度提供关于分析物离子的质子亲和力的附加信息。 由于离子化等离子体中的质子供体的数量的增加导致具有较低质子亲和力的分析物质的电离增加，所以浓度精度得到提高。

公开(公告)号：US07893402B2

公开(公告)日：2011-02-22

申请号：US12052267

申请日：2008-03-20

Applicant: Ian Sanders ,  Gökhan Baykut ,  Jochen Franzen

Inventor： Ian Sanders ,  Gökhan Baykut ,  Jochen Franzen

IPC: B01D59/44

CPC classification number: H01J49/004 ,  G01N27/622

Abstract: The mobility of mass-selected ions in gases is measured at pressures of a few hectopascal by selecting the ions under investigation in a quadrupole filter according to their mass-to-charge ratio m/z, measuring their mobility in a drift region at a pressure of a few hundred Pascal under the influence of a DC electric field and then filtering the measured ions by means of a quadrupole field in order to eliminate, or detect changes in, the mass-to-charge ratio. Several embodiments for the drift region are disclosed, in which the ions are kept in the axis of the drift region by RF fields. As these drift regions can also be utilized for a collision-induced decomposition of the ions, the device can additionally be used as a so-called triple quadrupole mass spectrometer.

Abstract translation: 质量选择的离子在气体中的迁移率通过在四极滤波器中根据其质荷比m / z选择正在研究的离子来测量几何百帕斯卡的压力，测量它们在压力下的漂移区域中的迁移率 在直流电场的影响下，几百帕斯卡，然后通过四极场过滤测量的离子，以消除或检测质荷比的变化。 公开了漂移区的几个实施例，其中通过RF场将离子保持在漂移区的轴上。 由于这些漂移区域也可以用于离子的碰撞诱导分解，因此该装置可另外用作所谓的三重四极杆质谱仪。

公开(公告)号：US20110036978A1

公开(公告)日：2011-02-17

申请号：US12862299

申请日：2010-08-24

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: B01D59/44

CPC classification number: H01J49/0422

Abstract: Ions entrained in a gas are transported into the vacuum system of an ion user, such as a mass spectrometer, from an ion source located outside the vacuum. The gas and ions pass through a nozzle that connects the ion source to the vacuum system and is shaped to form a supersonic gas jet in a first vacuum chamber of the vacuum system. In the first vacuum chamber, ions entrained in the supersonic gas jet are extracted electrically or magnetically and are collected, for example, by an RF ion funnel and transmitted to the ion user. The supersonic gas jet travels on and, after passing through the first vacuum chamber, the supersonic gas jet is directed into a separate pump chamber out of which the gas is pumped.

Abstract translation: 携带在气体中的离子从位于真空外部的离子源转移到离子用户（例如质谱仪）的真空系统中。 气体和离子通过将离子源连接到真空系统的喷嘴，并被成形为在真空系统的第一真空室中形成超音速气体射流。 在第一真空室中，夹带在超声波气体射流中的离子被电或磁性地提取，并且例如通过RF离子漏斗收集并传输给离子用户。 超音速气体射流在通过第一真空室之后行进，超音速气体射流被引导到单独的泵室中，气体被泵送。

公开(公告)号：US07718959B2

公开(公告)日：2010-05-18

申请号：US11843753

申请日：2007-08-23

Applicant: Jochen Franzen ,  Gökhan Baykut

Inventor： Jochen Franzen ,  Gökhan Baykut

IPC: H01J49/42

CPC classification number: H01J49/4225 ,  H01J49/063 ,  H01J49/4295

Abstract: The invention relates to instruments for storing ions in more than one ion storage device and to the use of the storage bank thus created. The ion storage bank includes several storage cells configured as RF multipole rod systems, where the cells contain damping gas and are arranged in parallel. Each pair of pole rods is used jointly by two immediately adjacent storage cells such that the ions collected can be transported from one storage cell to the next by briefly applying DC or AC voltages to individual pairs of pole rods. The ions can thus be transported to storage cells in which they are fragmented or reactively modified, or from which they can be fed to other spectrometers. In particular, a circular arrangement of the storage cells on a virtual cylindrical surface makes it possible to accumulatively fill the storage cells with ions of specific fractions from temporally sequenced separation runs.

Abstract translation: 本发明涉及用于将离子存储在多于一个离子存储装置中的仪器以及由此产生的储存库的使用。 离子存储库包括配置为RF多极杆系统的多个存储单元，其中单元包含阻尼气体并且并联布置。 每对极杆由两个紧邻的存储单元联合使用，使得通过向单个极杆对简单地施加DC或AC电压，所收集的离子可以从一个存储单元传送到下一个存储单元。 因此，离子可以被运送到它们被分段或反应性修饰的存储单元中，或者从这些离子将它们送入其它光谱仪。 特别地，在虚拟圆柱形表面上的存储单元的圆形布置使得可以从时间上排序的分离行程累积地填充具有特定级分的离子的存储单元。