公开(公告)号：US08765433B2

公开(公告)日：2014-07-01

申请号：US12980597

申请日：2010-12-29

申请人： Sriram Satagopan ,  Daniel P. O'Keefe ,  Janardhan Gude

发明人： Sriram Satagopan ,  Daniel P. O'Keefe ,  Janardhan Gude

IPC分类号： C12N9/04 ,  C12P7/16 ,  C12N1/19

CPC分类号： C12P7/16 ,  C12N9/0004 ,  C12N15/81 ,  Y02E50/10 ,  Y02P20/52

摘要： The invention relates to suitable candidate alcohol dehydrogenase (ADH) enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same.

摘要翻译： 本发明涉及用于生产低级烷基醇（包括异丁醇）的合适的候选醇脱氢酶（ADH）酶。 本发明还涉及包含这种ADH酶的重组宿主细胞及其制备其中的低级烷基醇的方法。

公开(公告)号：US08759044B2

公开(公告)日：2014-06-24

申请号：US13525075

申请日：2012-06-15

申请人： Robert DiCosimo ,  Arthur Leo Kruckeberg ,  Thomas Edwin Van Aken

发明人： Robert DiCosimo ,  Arthur Leo Kruckeberg ,  Thomas Edwin Van Aken

IPC分类号： C12P7/62 ,  C12P7/16

CPC分类号： C12N15/81 ,  A23K10/10 ,  A23K10/18 ,  C12N9/20 ,  C12P7/06 ,  C12P7/16 ,  C12P7/62 ,  C12P7/6436 ,  Y02E50/10 ,  Y02E50/17

摘要： Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.

摘要翻译： 本文公开了在发酵期间通过提供产生醇的微生物来生产醇酯的方法，其进一步包含编码具有脂肪酶活性的多肽的工程化多核苷酸。

公开(公告)号：US20140162344A1

公开(公告)日：2014-06-12

申请号：US14180811

申请日：2014-02-14

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： Robert DICOSIMO ,  Arthur Leo Kruckeberg ,  Thomas Edwin Van Aken

IPC分类号： C12N15/52

CPC分类号： C12N15/52 ,  C12P7/06 ,  C12P7/16 ,  C12P7/6436 ,  Y02E50/10 ,  Y02E50/17

摘要： Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.

公开(公告)号：US20140142352A1

公开(公告)日：2014-05-22

申请号：US14084881

申请日：2013-11-20

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： MICHAEL DAUNER ,  Robert Dicosimo ,  Steven D. Doig ,  Adam David Henry ,  Joseph J. Zaher

IPC分类号： C12P7/16 ,  C07C31/12

CPC分类号： C12P7/16 ,  C07C31/12 ,  Y02E50/10

摘要： Provided herein are processes for adjusting a fermentation medium to reduce the activity of one or more carboxylic acids. The processes comprise (a) providing a recombinant microorganism comprising an engineered butanol biosynthetic pathway, (b) contacting the recombinant microorganism with a fermentation medium whereby butanol is produced and wherein the fermentation medium comprises one or more carboxylic acids, and (c) adjusting the fermentation medium to reduce the activity of the one or more carboxylic acids. Also provided are processes for reducing the activity of one or more carboxylic acids in a feed. The processes comprise (a) providing a feed from a fermentation vessel, wherein the feed comprises a composition produced by a recombinant microorganism comprising an engineered butanol biosynthetic pathway, wherein the composition comprises butanol, water, and one or more carboxylic acids; and (b) adjusting the feed, wherein adjusting the feed reduces the activity of the one or more carboxylic acids.

摘要翻译： 本文提供了调节发酵培养基以降低一种或多种羧酸的活性的方法。 所述方法包括（a）提供包含工程改造的丁醇生物合成途径的重组微生物，（b）使重组微生物与发酵培养基接触，由此产生丁醇，其中发酵培养基包含一种或多种羧酸，和（c） 发酵培养基以降低一种或多种羧酸的活性。 还提供了降低进料中一种或多种羧酸的活性的方法。 所述方法包括（a）提供来自发酵容器的进料，其中所述进料包含由包含工程丁醇生物合成途径的重组微生物产生的组合物，其中所述组合物包含丁醇，水和一种或多种羧酸; 和（b）调节进料，其中调节进料降低了一种或多种羧酸的活性。

公开(公告)号：US08697404B2

公开(公告)日：2014-04-15

申请号：US13326660

申请日：2011-12-15

申请人： Douglas Robert Anton ,  Michael Dauner ,  Robert Dicosimo

发明人： Douglas Robert Anton ,  Michael Dauner ,  Robert Dicosimo

IPC分类号： C12P7/64

CPC分类号： C12P7/04 ,  A23K10/12 ,  A23K10/38 ,  C12N9/18 ,  C12P7/06 ,  C12P7/16 ,  C12P7/18 ,  C12P7/62 ,  C12P7/649 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/17 ,  Y02P60/873

摘要： Diols produced in fermentation are processed in broth by esterification of the product diol with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product diol, such as 1,3-propanediol, with the carboxylic acid to form the diol esters. The diol esters can be extracted from the broth, and the product diol recovered from the diol esters. The carboxylic acid can also serve as an extractant for removal of the diol esters from the fermentation medium.

摘要翻译： 在发酵中产生的二醇通过产物二醇与羧酸（例如脂肪酸）和能够使产物二醇如1,3-丙二醇与羧酸酯化的催化剂（例如脂肪酶）酯化而在肉汤中加工。 酸形成二醇酯。 可以从肉汤中提取二醇酯，从二醇酯中回收产物二醇。 羧酸也可用作从发酵培养基中除去二醇酯的提取剂。

公开(公告)号：US20130316414A1

公开(公告)日：2013-11-28

申请号：US13872873

申请日：2013-04-29

申请人： Butamax Advanced Biofuels LLC

发明人： Brian James PAUL

IPC分类号： C12N15/74

CPC分类号： C12N15/746 ,  C12N9/0006 ,  C12P7/16 ,  C12P7/18 ,  C12P7/26 ,  C12Y101/02004 ,  Y02E50/10

摘要： A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.

摘要翻译： 通过基因工程实现了植物乳杆菌中从丙酮酸到2,3-丁二醇的高通量代谢物。 在异源表达的丁二醇脱氢酶活性的存在下，大量消除乳酸脱氢酶活性导致2,3-丁二醇的产生，其至少是丙酮酸衍生产物总数的49％。

公开(公告)号：US20130247450A1

公开(公告)日：2013-09-26

申请号：US13895522

申请日：2013-05-16

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： Leslie R. Wolf

IPC分类号： C10L1/232 ,  C10L1/182 ,  C10L1/224 ,  C10L1/228 ,  C10L1/226

CPC分类号： C10L1/22 ,  C07C249/02 ,  C10L1/026 ,  C10L1/1824 ,  C10L1/221 ,  C10L1/2222 ,  C10L1/224 ,  C10L1/226 ,  C10L1/228 ,  C10L1/2283 ,  C10L1/232 ,  C10L1/238 ,  C10L1/2383 ,  C10L1/2387 ,  C10L10/18

摘要： A gasoline deposit control additive composition for use in a fuel comprising from about 70 to about 95 volume percent of hydrocarbons in the gasoline boiling range and from about 5 to about 30 volume percent of at least one alcohol, comprising the imine or tertiary amine product of the reaction between (a) at least one aldehyde or ketone or mixture thereof having the formula R16CHO, R16CH2CHO, R17(C=0)R18 or R17CH2(C=0)R18, wherein R16, R17, and R18 are the same or different and are each independently a straight or branched chain hydrocarbyl or aryl group that contains from 1 to 18 carbon atoms, and (b) a primary or secondary amine functionality.

摘要翻译： 一种用于燃料的汽油沉积物控制添加剂组合物，其包含约70至约95体积％的汽油沸程中的烃和约5至约30体积％的至少一种醇，其包含亚胺或叔胺产物 （a）具有式R 16 CHO，R 16 CH 2 CHO，R 17（C = O）R 18或R 17 CH 2（C = O）R 18的至少一种醛或酮或其混合物之间的反应，其中R16，R17和R18相同或不同 并且各自独立地为含有1至18个碳原子的直链或支链烃基或芳基，和（b）伯胺或仲胺官能团。

公开(公告)号：US08476047B2

公开(公告)日：2013-07-02

申请号：US13692254

申请日：2012-12-03

申请人： Keith H. Burlew ,  Robert Dicosimo ,  Michael Charles Grady

发明人： Keith H. Burlew ,  Robert Dicosimo ,  Michael Charles Grady

IPC分类号： C12P7/02 ,  C12P7/16

CPC分类号： C12P7/16 ,  A23K10/38 ,  B01D3/002 ,  B01D11/0492 ,  B01D21/26 ,  B01D21/262 ,  C07C29/86 ,  C11B1/025 ,  C11B13/00 ,  C12F3/10 ,  C12M1/00 ,  C12M21/12 ,  C12M45/04 ,  C12M45/09 ,  C12N1/14 ,  C12N15/00 ,  C12P7/18 ,  C12P7/6418 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02W30/74

摘要： In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol.

摘要翻译： 在酒精发酵过程中，将来自生物质的油水解成可从发酵液中原位除去产物醇如丁醇的萃取剂。 油中的甘油酯可以催化（例如，酶促）水解成游离脂肪酸，其形成产物醇的分配系数大于产物醇的生物质油的分配系数的发酵产物萃取剂。 由酒精发酵方法的原料得到的油可以通过使包含油的原料与一种或多种酶接触来水解，由此至少一部分油被水解成形成发酵产物萃取剂的游离脂肪酸，或者油可以是 在原料进料到发酵容器之前与原料分离，并且分离的油可以与酶接触以形成发酵产物萃取剂。 发酵产物萃取剂可与发酵液接触，用于原位除去产物醇。

公开(公告)号：US08465560B1

公开(公告)日：2013-06-18

申请号：US12697590

申请日：2010-02-01

申请人： Leslie R. Wolf

发明人： Leslie R. Wolf

IPC分类号： C10L1/22

CPC分类号： C10L1/22 ,  C07C249/02 ,  C10L1/026 ,  C10L1/1824 ,  C10L1/221 ,  C10L1/2222 ,  C10L1/224 ,  C10L1/226 ,  C10L1/228 ,  C10L1/2283 ,  C10L1/232 ,  C10L1/238 ,  C10L1/2383 ,  C10L1/2387 ,  C10L10/18

摘要： A gasoline deposit control additive composition for use in a fuel comprising from about 70 to about 95 volume percent of hydrocarbons in the gasoline boiling range and from about 5 to about 30 volume percent of at least one alcohol, comprising the imine or tertiary amine product of the reaction between (a) at least one aldehyde or ketone or mixture thereof having the formula R16 CHO, R16 CH2 CHO, R17 (C=0) R18 or R17 CH2 (C=0) R18, wherein R16, R17, and R18 are the same or different and are each independently a straight or branched chain hydrocarbyl or aryl group that contains from 1 to 18 carbon atoms, and (b) a primary or secondary amine functionality.

摘要翻译： 一种用于燃料的汽油沉积物控制添加剂组合物，其包含约70至约95体积％的汽油沸程中的烃和约5至约30体积％的至少一种醇，其包含亚胺或叔胺产物 （a）至少一种具有式R 16 CHO，R 16 CH 2 CHO，R 17（C = O）R 18或R 17 CH 2（C = O）R 18的醛或酮或其混合物或其混合物之间的反应，其中R16，R17和R18为 相同或不同，并且各自独立地为含有1至18个碳原子的直链或支链烃基或芳基，和（b）伯胺或仲胺官能团。

公开(公告)号：US10604774B2

公开(公告)日：2020-03-31

申请号：US15799466

申请日：2017-10-31

申请人： Butamax Advanced Biofuels LLC

发明人： Larry Cameron Anthony ,  Tyler T. Ames ,  Keith H. Burlew

IPC分类号： C12P7/16 ,  C10L1/18 ,  C12N9/10 ,  C12N9/88 ,  C12P5/00 ,  F02D19/08 ,  C10L1/183

摘要： Provided herein are processes and microorganisms which utilize both protein hydrolysates and carbohydrates from biomass feedstocks to produce renewable hydrocarbon compositions. Advantages of the disclosed methods may be recognized in fuel blends comprising such hydrocarbon compositions.