公开(公告)号：US20140227736A1

公开(公告)日：2014-08-14

申请号：US14239980

申请日：2012-08-21

申请人： Timothy J. Nelson ,  Andre Terzic ,  Almudena J. Martinez Fernandez ,  Clifford D. Folmes

发明人： Timothy J. Nelson ,  Andre Terzic ,  Almudena J. Martinez Fernandez ,  Clifford D. Folmes

IPC分类号： C12N5/074 ,  G01N33/50

CPC分类号： C12N5/0696 ,  C07H21/04 ,  C12N15/63 ,  C12N2500/34 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/00 ,  C12N2506/1307 ,  C12N2510/00 ,  G01N33/5005

摘要： This document provides methods and materials involved obtaining induced pluripotent stem (iPS) cells. For example, methods and materials for increasing the efficiency for making iPS cells as well as methods and materials for selecting iPS cells are provided.

摘要翻译： 本文件提供了获得诱导多能干细胞（iPS）细胞的方法和材料。 例如，提供了用于提高制备iPS细胞的效率的方法和材料以及用于选择iPS细胞的方法和材料。

公开(公告)号：US20140206083A1

公开(公告)日：2014-07-24

申请号：US14195698

申请日：2014-03-03

申请人： KYOTO UNIVERSITY

发明人： Kazuhiro Sakurada ,  Tetsuya Ishikawa ,  Hideki Masaki ,  Shunichi Takahashi

IPC分类号： C12N15/85

CPC分类号： C12N15/85 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00 ,  C12N2799/027

摘要： Establishment of human pluripotent stem cells having properties close to human ES cells with the genome of the patient per se that can circumvent immunological rejection of transplanted cells from cells derived from a postnatal human tissue are described. Human pluripotent stem cells can be induced by introducing three genes of Oct3/4, Sox2 and Klf 4, or three genes of Oct3/4, Sox2 and Klf 4 plus the c-Myc gene or a histone deacetylase (HDAC) inhibitor of undifferentiated stem cells present in various human postnatal tissues in which each gene of Tert, Nanog, Oct3/4 and Sox2 has not undergone epigenetic inactivation.

摘要翻译： 描述了具有接近人类ES细胞的性质的人多能干细胞的建立，所述人类多能干细胞具有患者本身的基因组，其可以规避来自出生后人体组织的细胞的移植细胞的免疫排斥。 可以通过引入Oct3 / 4，Sox2和Klf4的三个基因或Oct3 / 4，Sox2和Klf4的三个基因加上c-Myc基因或未分化茎的组蛋白脱乙酰酶（HDAC）抑制剂来诱导人多能干细胞 存在于各种人类出生后组织中的细胞，其中Tert，Nanog，Oct3 / 4和Sox2的每个基因未经历表观遗传学失活。

公开(公告)号：US08716020B2

公开(公告)日：2014-05-06

申请号：US13505934

申请日：2010-11-10

申请人： Philippe Mangeot ,  Vincent Lotteau ,  Marc Peschanski ,  Mathilde Girard

发明人： Philippe Mangeot ,  Vincent Lotteau ,  Marc Peschanski ,  Mathilde Girard

IPC分类号： C12N5/07 ,  C12N5/073

CPC分类号： C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608

摘要： The present invention relates to a non-genetic, detergent-free, bacteria-free method for reprogramming a eukaryotic cell, in particular for obtaining induced pluripotent stem cells (iPS), by using engineered microvesicles carrying at least one reprogramming transcription factor, wherein said engineered microvesicles are virus-free.

摘要翻译： 本发明涉及通过使用携带至少一种重编程转录因子的工程化微泡来重编程真核细胞，特别是用于获得诱导性多能干细胞（iPS）的非遗传，无去污剂，无细菌的方法，其中所述 工程微泡是无病毒的。

公开(公告)号：US20130344602A1

公开(公告)日：2013-12-26

申请号：US13931251

申请日：2013-06-28

申请人： Factor Bioscience, Inc.

发明人： Matthew Angel ,  Christopher Rohde

IPC分类号： C12N5/00

CPC分类号： C12N5/0696 ,  C12N5/0018 ,  C12N5/0625 ,  C12N5/0647 ,  C12N5/0656 ,  C12N5/0657 ,  C12N5/0676 ,  C12N5/0695 ,  C12N2500/25 ,  C12N2500/33 ,  C12N2500/36 ,  C12N2500/84 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/998 ,  C12N2506/09 ,  C12N2506/45

摘要： The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.

摘要翻译： 本发明部分地涉及从患者样品中产生组织特异性细胞的方法以及使用这些方法产生的组织特异性细胞。 公开了使用RNA重编程细胞的方法。 还公开了包含使用这些方法生产的细胞的治疗剂。

公开(公告)号：US20130323833A1

公开(公告)日：2013-12-05

申请号：US13896259

申请日：2013-05-16

申请人： The Scripps Research Institute

发明人： Saiyong Zhu ,  Sheng Ding

IPC分类号： C12N5/074

CPC分类号： C12N5/0696 ,  C12N2501/065 ,  C12N2501/15 ,  C12N2501/41 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2506/03 ,  C12N2506/094 ,  C12N2506/28

摘要： The present invention provides for methods, compositions, and kits for producing an induced pluripotent stem cell from a non-pluripotent mammalian cell using a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator or a compound that promotes glycolytic metabolism as well as other small molecules.

摘要翻译： 本发明提供用于使用3'-磷酸肌醇依赖性激酶-1（PDK1）激活剂或促进糖酵解代谢的化合物从非多能哺乳动物细胞产生诱导多能干细胞的方法，组合物和试剂盒，以及 其他小分子。

公开(公告)号：US20130266541A1

公开(公告)日：2013-10-10

申请号：US13441328

申请日：2012-04-06

申请人： Elias T. Zambidis ,  Tea Soon Park

发明人： Elias T. Zambidis ,  Tea Soon Park

IPC分类号： C12N5/10 ,  A61K35/12

CPC分类号： C12N5/0696 ,  A61K35/545 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1369 ,  C12N2510/00

摘要： The present invention relates generally to the field of stem cells and, more particularly, to reprogramming blood cells to pluripotent stem cells. In a specific embodiment, a method for producing an induced pluripotent stem cell from a human myeloid progenitor cell comprising the steps of (a) activating the human myeloid progenitor cell by incubation with hematopoietic growth factors; (b) transfecting the activated progenitor cells with a non-viral vector expressing one or more pluripotency factors; and (c) co-culturing the transfected cells with irradiated mesenchymal bone marrow stromal cells.

摘要翻译： 本发明一般涉及干细胞领域，更具体地涉及将血细胞重编程为多能干细胞。 在一个具体实施方案中，一种从人骨髓祖细胞产生诱导多能干细胞的方法，包括以下步骤：（a）通过与造血生长因子温育来激活人骨髓祖细胞; （b）用表达一种或多种多能因子的非病毒载体转染活化的祖细胞; 和（c）用经照射的间充质骨髓基质细胞共培养转染的细胞。

公开(公告)号：US20130034900A1

公开(公告)日：2013-02-07

申请号：US13505934

申请日：2010-11-10

申请人： Philippe Mangeot ,  Vincent Lotteau ,  Marc Peschanski ,  Mathilde Girard

发明人： Philippe Mangeot ,  Vincent Lotteau ,  Marc Peschanski ,  Mathilde Girard

IPC分类号： C12N5/07 ,  C12N5/073

CPC分类号： C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608

摘要： The present invention relates to a non-genetic, detergent-free, bacteria-free method for reprogramming a eukaryotic cell, in particular for obtaining induced pluripotent stem cells (iPS), by using engineered microvesicles carrying at least one reprogramming transcription factor, wherein said engineered microvesicles are virus-free.

摘要翻译： 本发明涉及通过使用携带至少一种重编程转录因子的工程化微泡来重编程真核细胞，特别是用于获得诱导性多能干细胞（iPS）的非遗传，无去污剂，无细菌的方法，其中所述 工程微泡是无病毒的。

公开(公告)号：US08257941B2

公开(公告)日：2012-09-04

申请号：US12484163

申请日：2009-06-12

申请人： Kazuhiro Sakurada ,  Kenneth J. Seidenman

发明人： Kazuhiro Sakurada ,  Kenneth J. Seidenman

IPC分类号： C12N15/00

CPC分类号： C12N15/85 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00 ,  C12N2799/027

摘要： The present invention involves methods for identifying an agent that corrects a phenotype associated with a health condition or a predisposition for a health condition. The invention also involves methods for identifying a diagnostic cellular phenotype, determining the risk of a health condition in a subject, methods for reducing the risk of drug toxicity in a human subject, and methods for identifying a candidate gene that contributes to a human disease. The invention also discloses human induced pluripotent stem cell lines.

摘要翻译： 本发明涉及用于鉴定校正与健康状况或健康状况的倾向相关联的表型的药剂的方法。 本发明还涉及用于鉴定诊断性细胞表型，确定受试者健康状况风险的方法，降低人类受试者中药物毒性风险的方法，以及用于鉴定对人类疾病有贡献的候选基因的方法。 本发明还公开了人诱导的多能干细胞系。

公开(公告)号：US20120220030A1

公开(公告)日：2012-08-30

申请号：US13391251

申请日：2010-08-18

申请人： Renee A. Reijo Pera ,  James Byrne

发明人： Renee A. Reijo Pera ,  James Byrne

IPC分类号： C12N5/071

CPC分类号： C12N5/0696 ,  C12N2500/44 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2502/13

摘要： A substantially pure population of human somatic cells that have enhanced potential to become induced pluripotent stem cells (iPS cells) is provided. Also provided are methods for generating this population of cells and methods for generating iPS cells from this population of cells.

摘要翻译： 提供了具有增强潜力成为诱导多能干细胞（iPS细胞）的基本上纯的人体细胞群体。 还提供了用于产生该细胞群的方法和用于从该细胞群体产生iPS细胞的方法。

公开(公告)号：US20120214234A1

公开(公告)日：2012-08-23

申请号：US13337767

申请日：2011-12-27

申请人： Tetsuro TAKAMATSU ,  Ping DAI

发明人： Tetsuro TAKAMATSU ,  Ping DAI

IPC分类号： C12N5/02 ,  C12N5/10 ,  C12N5/00

CPC分类号： C12N5/0696 ,  C12N5/0606 ,  C12N5/0678 ,  C12N2310/14 ,  C12N2500/99 ,  C12N2501/10 ,  C12N2501/15 ,  C12N2501/19 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2506/1315 ,  C12N2506/45

摘要： The present invention provides a method for producing iPS cells, comprising reacting cells with at least one connexin inhibitor and at least one TGFβ signaling inhibitor; iPS cells comprising at least one connexin inhibitor; an iPS cell inducer comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGFβ signaling inhibitors; a medium for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGFβ signaling inhibitors; and a kit for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGFβ signaling inhibitors.

摘要翻译： 本发明提供了一种生产iPS细胞的方法，包括使细胞与至少一种连接蛋白抑制剂和至少一种TGFβb反应; 信号传导抑制剂; 包含至少一种连接蛋白抑制剂的iPS细胞; iPS细胞诱导剂，其包含至少一种选自连接蛋白抑制剂和TGFβb的抑制剂; 信号传导抑制剂; 用于诱导iPS细胞的培养基，其包含至少一种选自连接蛋白抑制剂和TGFβb的抑制剂; 信号传导抑制剂; 以及用于诱导iPS细胞的试剂盒，其包含至少一种选自连接蛋白抑制剂和TGFβb的抑制剂; 信号传导抑制剂。