公开(公告)号：US5545304A

公开(公告)日：1996-08-13

申请号：US441319

申请日：1995-05-15

申请人： Richard D. Smith ,  Jon H. Wahl ,  Steven A. Hofstadler

发明人： Richard D. Smith ,  Jon H. Wahl ,  Steven A. Hofstadler

IPC分类号： G01N27/447 ,  H01J49/04 ,  B01D59/44 ,  G01N27/26

CPC分类号： H01J49/025 ,  G01N27/44717 ,  G01N27/4473

摘要： The present invention relates generally to any application involving the monitoring of signal arising from ions produced by electrospray or other high pressure (>100 torr) ion sources. The present invention relates specifically to an apparatus and method for the detection of ions emitted from a capillary electrophoresis (CE) system, liquid chromatography, or other small-scale separation methods. And further, the invention provides a very simple diagnostic as to the quality of the separation and the operation of an electrospray source.

摘要翻译： 本发明一般涉及涉及监测由电喷雾或其它高压（> 100托）离子源产生的离子产生的信号的任何应用。 本发明具体涉及用于检测从毛细管电泳（CE）系统，液相色谱法或其他小规模分离方法发射的离子的装置和方法。 此外，本发明提供了关于电喷雾源的分离和操作的质量的非常简单的诊断。

公开(公告)号：US5472584A

公开(公告)日：1995-12-05

申请号：US406764

申请日：1995-03-17

申请人： Roy D. Rocklin ,  Christopher A. Pohl ,  John Stillian ,  Nebojsa Avdalovic

发明人： Roy D. Rocklin ,  Christopher A. Pohl ,  John Stillian ,  Nebojsa Avdalovic

IPC分类号： G01N27/447 ,  G01N30/60 ,  G01N30/96 ,  G01N27/26

CPC分类号： G01N27/4473 ,  G01N2030/965

摘要： Apparatus and methods are provided for increasing the sensitivity of detection of ionic species separated by capillary electrophoresis. The apparatus includes capillary electrophoretic separating means, suppressor means and detector means.

摘要翻译： 提供了提高通过毛细管电泳分离的离子物质检测灵敏度的装置和方法。 该装置包括毛细管电泳分离装置，抑制装置和检测装置。

公开(公告)号：US5358612A

公开(公告)日：1994-10-25

申请号：US771597

申请日：1991-10-04

申请人： Purnendu K. Dasgupta ,  Bao Li-Yuan

发明人： Purnendu K. Dasgupta ,  Bao Li-Yuan

IPC分类号： G01N27/26 ,  G01N27/447 ,  G01N30/60 ,  G01N30/96

CPC分类号： G01N27/4473 ,  G01N2030/965

摘要： Improved capillary electrophoresis apparatus of the type that generally includes a capillary tube, the capillary tube having a sample inlet end and an outlet end, a first electrode in electrical communication with the inlet end of the capillary tube, a second electrode in electrical communication with the outlet end of the capillary tube and a high voltage power supply in electrical communication with the first and second electrodes. The improvement is to connect a miniature Ion Chromatography membrane suppressor to the outlet end of the capillary tube, to connect a conductivity detector to the membrane suppressor and to place the second electrode in the regenerant compartment of the membrane suppressor. The method embodiment of the invention for anion analysis includes the steps of: (a) separating anions of interest by capillary electrophoresis in a buffer solution; (b) exchanging cations of the buffer for regenerant cations using an ion chromatography membrane suppressor to reduce the electrical conductivity of the buffer to produce a suppressed buffer; and (c) measuring the electrical conductivity of the suppressed buffer to determine the separated anions. Similarly, the method embodiment of the invention for cation analysis includes the steps of: (a) separating cations of interest by capillary electrophoresis in a buffer solution; (b) exchanging anions of the buffer for regenerant anions using a stationary means for exchanging anions thereby reducing the electrical conductivity of the buffer to produce a suppressed buffer; and (c) measuring the electrical conductivity of the suppressed buffer to determine the separated cations.

摘要翻译： 改进的毛细管电泳装置通常包括毛细管，毛细管具有样品入口端和出口端，与毛细管的入口端电连通的第一电极，与第二电极电连通的第二电极 毛细管的出口端和与第一和第二电极电连通的高压电源。 改进之处是将微型离子色谱膜抑制器连接到毛细管的出口端，将电导率检测器连接到膜抑制器上，并将第二电极放置在膜抑制器的再生室中。 用于阴离子分析的本发明的方法实施方案包括以下步骤：（a）通过毛细管电泳在缓冲溶液中分离感兴趣的阴离子; （b）使用离子色谱膜抑制剂交换用于再生阳离子的缓冲液的阳离子，以降低缓冲液的电导率以产生抑制缓冲液; 和（c）测量抑制缓冲液的电导率以确定分离的阴离子。 类似地，用于阳离子分析的本发明的方法实施例包括以下步骤：（a）通过毛细管电泳在缓冲溶液中分离目标阳离子; （b）使用用于交换阴离子的固定装置交换用于再生阴离子的缓冲液的阴离子，从而降低缓冲液的电导率以产生抑制缓冲液; 和（c）测量抑制缓冲液的电导率以确定分离的阳离子。

公开(公告)号：US5340449A

公开(公告)日：1994-08-23

申请号：US965862

申请日：1992-10-23

申请人： Ashok K. Shukla

发明人： Ashok K. Shukla

IPC分类号： B01D57/02 ,  B01D61/28 ,  B01D61/42 ,  B01F13/08 ,  C12N15/10 ,  G01N1/34 ,  G01N27/447

CPC分类号： B01D61/422 ,  B01D57/02 ,  B01D61/28 ,  B01D61/425 ,  B01F13/0818 ,  C12N15/101 ,  G01N27/4473 ,  G01N2001/4016

摘要： This invention involves the elution of macromolecules such as proteins, deoxyribonucleic acid(DNA), ribonucleic acid(RNA) from solid phase matrix such as polyacrylamide, agarose and membranes such as polyvinyl difluoride (PVDF) in an electric field. The elution chamber shown in FIG. 1 consists of two separate screws 9, 9a and attachable chamber 1. The screw well 8 holds the gel matrix containing the macromolecules and is separated by the bottom chamber 1 with a membrane 5 with a nominal molecular weight cutoff higher than the macromolecule. On the other hand, the macromolecule is retained in the well 1 by introducing another membrane 6 having a molecular weight cutoff lower than the molecular weight of the macromolecule. Alternatively, macromolecules separated on membrane, e.g. PVDF, are held in a Teflon or plastic membrane holder 11. This holder can fit inside well 8 in screw 9. In addition, chamber 1 contains a well 7. The macromolecules migrate through the electrophoretic field and are concentrated in well 7. Since the volume of the well 7 is predetermined (10 ul--one ml or more), results in concentration of the macromolecules. Next, the chamber 1 is unscrewed from the top screw 8 and closed with a screw without a hole 13, and the entire device is submerged into a dialysis buffer. Following dialysis for a given time, the fluid above the bottom screw 9a itself and the membrane beneath it are removed. The sample is taken out from hole 7. If further concentration of the sample is required, then following removal of the fluid above the screw 9a, the entire device is transferred to a vacuum desiccator and concentrated to any desired volume.

摘要翻译： 本发明涉及在电场中从固相基质如聚丙烯酰胺，琼脂糖和膜如聚二氟乙烯（PVDF）等洗脱大分子如蛋白质，脱氧核糖核酸（DNA），核糖核酸（RNA）。 图1所示的洗脱室。 1由两个单独的螺钉9,9a和可连接室1组成。螺旋孔8保持含有大分子的凝胶基质，并由底部室1与膜5隔离，其中标称分子量截止值高于大分子。 另一方面，通过引入分子量低于高分子分子量的另一膜6，将大分子保留在孔1中。 或者，在膜上分离的大分子，例如 PVDF被保持在特氟隆或塑料膜保持器11中。该保持器可以装配在螺钉9中的孔8内。另外，腔室1包含孔7.大分子迁移通过电泳场并且集中在井7.由于 孔7的体积是预定的（10ul-1ml或更大），导致大分子的浓度。 接下来，将腔室1从顶部螺钉8拧下，并用不带孔13的螺丝封闭，并将整个装置浸入透析缓冲液中。 在给定时间透析后，去除底部螺丝9a本身上方的流体和其下面的膜。 样品从孔7中取出。如果需要进一步浓缩样品，则在除去螺杆9a上方的流体之后，将整个装置转移至真空干燥器并浓缩至任何所需体积。

公开(公告)号：US5245185A

公开(公告)日：1993-09-14

申请号：US787934

申请日：1991-11-05

申请人： Kenneth L. Busch ,  Jocelyn C. Dunphy

发明人： Kenneth L. Busch ,  Jocelyn C. Dunphy

IPC分类号： G01N27/447 ,  H01J49/04

CPC分类号： H01J49/164 ,  G01N27/44717 ,  G01N27/4473 ,  H01J49/0431

摘要： An introduction means for the direct introduction of a sample taken from a planar electrophoresis into an interface to a mass spectrometer utilizing laser desorption ionization. The introduction means includes a probe to collect a sample from a planar electrophoresis, generally a gel electrophoresis, comprising a dual electrode assembly and a potential gradient between the electrodes to collect the sample. Alternatively, the sample is obtained from a freeze-squeeze method or a drying/matrix-deposition method.

摘要翻译： 引入装置，用于使用激光解吸电离将从平面电泳获取的样品直接引入到质谱仪的界面。 引入装置包括从平面电泳（通常是凝胶电泳）收集样品的探针，其包括双电极组件和电极之间的电势梯度以收集样品。 或者，样品由冷冻挤压法或干燥/基质沉积法获得。

公开(公告)号：US5173159A

公开(公告)日：1992-12-22

申请号：US805138

申请日：1991-12-11

申请人： Bernard Dutertre

发明人： Bernard Dutertre

IPC分类号： B01D57/02 ,  C07K1/26 ,  G01N27/447

CPC分类号： C07K1/26 ,  B01D57/02 ,  G01N27/4473 ,  G01N27/44773

摘要： A multiple electrophoresis method and device are disclosed for the controlled migration of macromolecules in gel plates stacked in a direction perpendicular to their plane, at least one series of electrodes (20) disposed at the intersections of two series of peripendicular planes, some perpendicular to the plates (12) and to the desired direction of migration of the macromolecules, the others perpendicular to the first and parallel to the plates (12), the electrodes being connected together selectively and controlled so as to provide both separation of the macromolecules in the plate (12) then transfer thereof to the membranes (22) associated with the plates (12).

摘要翻译： 公开了一种多电泳方法和装置，用于在垂直于它们的平面的方向堆叠的凝胶板中的大分子的受控迁移，至少一系列电极（20），设置在两系列垂直平面的交点处，一些垂直于 板（12）和所需的大分子的迁移方向，其它垂直于第一并平行于板（12）的电极，电极被选择性地连接在一起并被控制，以便提供板中的大分子的两个分离 （12）然后将其转移到与板（12）相关联的膜（22）上。

公开(公告)号：US4164464B1

公开(公告)日：1992-05-12

申请号：US89637078

申请日：1978-04-14

申请人： INSTRUMENTATION SPECIALTIES CO

发明人： WILLIAM B ALLINGTON ,  JAMES W NELSON ,  ARTHUR L CORDRY ,  GAIL A MCCULLOUGH ,  DON E MITCHELL

IPC分类号： B01D57/02 ,  G01N1/34 ,  G01N1/40 ,  G01N27/447 ,  G01N27/26 ,  G01N27/28

CPC分类号： G01N27/4473 ,  B01D57/02 ,  G01N1/34 ,  G01N2001/4016 ,  G01N2001/4038

公开(公告)号：US5102518A

公开(公告)日：1992-04-07

申请号：US590047

申请日：1990-09-27

申请人： Don S. Doering ,  Paul T. Matsudaira

发明人： Don S. Doering ,  Paul T. Matsudaira

IPC分类号： G01N27/447

CPC分类号： G01N27/4473

摘要： An electroelutor provides electroelution and concentration of small amounts of proteins from sample gels. The electroelutor elutes desired molecules from gels in a sample holder directly onto a membrane of a centrifuge concentrator or a sequencing membrane. After electroelution, the eluted molecules can be concentrated or processed in a sequencing device with minimized sample handling and minimized disturbance abouFUNDINGWork described herein relating to the present invention has been funded under contract no. DK35 306 awarded by the National Institutes of Health.

摘要翻译： 电焊膏提供电洗脱和浓缩来自样品凝胶的少量蛋白质。 电致变色器将所需分子从样品架中的凝胶中直接洗脱到离心浓缩器或测序膜的膜上。 电洗脱后，洗脱的分子可以在测序装置中进行浓缩或加工，使样品处理最小化，并减少对膜的干扰。

公开(公告)号：US4889606A

公开(公告)日：1989-12-26

申请号：US259646

申请日：1988-10-19

申请人： Nicholas J. Dyson ,  David P. Pashby ,  Judith M. Parke

发明人： Nicholas J. Dyson ,  David P. Pashby ,  Judith M. Parke

IPC分类号： B01D57/02 ,  G01N27/447

CPC分类号： G01N27/4473

摘要： A method and apparatus for use in biological testing. The apparatus comprises electrophoresis apparatus comprising a tank (1) the interior of which is formed with a raised portion (5) which splits the tank into the two compartments (6,7) containing electrophoresis electrolyte. Electrodes (8) are provided in each compartment. Electrophoresis is carried out through a gel (not shown) supported by a rigid porous plate (11). The gel is cast onto the porous plate with a transfer membrane (not shown) between the porous plate and the gel. Further electrodes (20,21) are mounted below and above the gel respectively. The method of the invention comprises a first stage using the electric field produced by the electrodes (8) to draw the samples through the gel and a second stage using the electric field produced by the electrodes (20,21) to draw the distributed macromolecules onto the transfer membrane.

公开(公告)号：US4608147A

公开(公告)日：1986-08-26

申请号：US637922

申请日：1984-08-06

申请人： Andreas Clad

发明人： Andreas Clad

IPC分类号： G01N27/26 ,  B01D57/02 ,  B01D61/44 ,  B01D61/46 ,  C07H21/00 ,  C07K1/26 ,  C08J5/18 ,  G01N27/28 ,  G01N27/447 ,  B01D13/02

CPC分类号： G01N27/4473 ,  B01D57/02 ,  G01N27/44704 ,  G01N27/44717

摘要： In an electro-elution apparatus for operation in an electrophoresis chamber and for the electro-elution of biological macromolecules from sections of electrophoresis gel, the macromolecules which are to be eluted are not temporarily adsorbed in a gel, but can be collected between two polymer membranes in a trap and pipetted out of the latter. The membrane, which is the first or inner membrane in the direction of migration of the macromolecules, is here permeable to the macromolecules under the action of the electric field, while the membrane, which is the second membrane in the direction of migration, namely the outer membrane of the electro-elution apparatus, is impermeable to the macromolecules under the same conditions. After the electric field has been switched off, both membranes are at least substantially impermeable to any mass transfer.

摘要翻译： 在用于在电泳室中操作的电洗脱装置和用于从电泳凝胶的部分电生物大分子的电洗脱中，待洗脱的大分子不会暂时吸附在凝胶中，而是可以收集在两个聚合物膜之间 在一个陷阱中，并从后者中吸出。 作为大分子迁移方向的第一膜或内膜的膜在电场作用下在这里对大分子是可渗透的，而作为迁移方向的第二膜的膜即是 电洗脱装置的外膜在相同条件下对大分子是不可渗透的。 在电场被关闭之后，两个膜至少对于任何传质都是不可渗透的。