公开(公告)号：US20120045788A1

公开(公告)日：2012-02-23

申请号：US13199104

申请日：2011-08-19

申请人： Ashok K. Shukla ,  Mukta M. Shukla

发明人： Ashok K. Shukla ,  Mukta M. Shukla

IPC分类号： C12Q1/02 ,  G01N33/68 ,  C08F10/00 ,  C08F14/26 ,  C08B3/06 ,  C08F112/08 ,  C08G75/23 ,  C08F10/06 ,  C08F10/02 ,  C08B1/00 ,  B01J8/00 ,  C08F212/10

CPC分类号： B01L3/0275 ,  B01J20/261 ,  B01J20/28016 ,  B01J20/28019 ,  B01J20/282 ,  B01J20/283 ,  B01J20/284 ,  B01J20/285 ,  B01J2220/445 ,  B01J2220/64 ,  B01L2300/0681 ,  C08L81/06 ,  G01N30/467 ,  G01N30/52 ,  G01N30/54 ,  G01N30/6091

摘要： A pipette tip or tube containing chromatographic media contained and held in place in said tube by using low melting point porous polymer particles. Such pipette tips or tubes can be used for sample preparation, filtration and synthesis of small molecules and biomolecules.

摘要翻译： 含有色谱介质的移液管尖端或管通过使用低熔点多孔聚合物颗粒包含并保持在所述管中的适当位置。 这种移液管吸头或管可用于样品制备，过滤和合成小分子和生物分子。

公开(公告)号：US06103195A

公开(公告)日：2000-08-15

申请号：US908931

申请日：1997-08-08

申请人： Ashok K. Shukla ,  Amita Shukla ,  Mukta Shukla

发明人： Ashok K. Shukla ,  Amita Shukla ,  Mukta Shukla

IPC分类号： G01N30/38 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/02

CPC分类号： G01N30/38 ,  G01N2030/381 ,  G01N2030/527 ,  G01N30/461 ,  G01N30/6065

摘要： This invention relates to micro volume spin columns used for the preparation and purification of small chemical and/or biological samples by centrifugation. The present invention relates to the use of the conventional luer lock fitting in micro volume spin columns. This invention also relates to multi-spin column systems based on joining two or more spin columns by their ends or by combining different filtration or ultra-filtration membranes with spin columns.

摘要翻译： 本发明涉及用于通过离心制备和纯化小型化学和/或生物样品的微量体积旋转柱。 本发明涉及传统的鲁尔锁配件在微体积旋转柱中的应用。 本发明还涉及通过其两端连接两个或更多个旋转塔或通过将不同的过滤或超滤膜与旋转塔结合的多旋转塔系统。

公开(公告)号：US5922186A

公开(公告)日：1999-07-13

申请号：US903456

申请日：1997-07-28

申请人： Ashok K. Shukla ,  Amita M. Shukla

发明人： Ashok K. Shukla ,  Amita M. Shukla

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44721 ,  G01N27/44704

摘要： The present invention presents a novel method for both staining electrophoresis gels, as well as removing the background dye during de-staining. This novel and simple pad-based technique minimizes solvent consumption, is environmentally friendly, and does not leave any solid residues on the gel. Furthermore, a novel method for the quantitative and qualitative determination of protein concentration in solution is also presented. This method is simple, easy-to-use and has an easily visible color change from clear to blue in the presence of protein.

摘要翻译： 本发明提供了一种染色电泳凝胶的新方法，以及脱色期间去除背景染料。 这种新颖简单的基于垫的技术可最大限度地减少溶剂消耗，是环保的，并且不会在凝胶上留下任何固体残留物。 此外，还提出了一种定量和定性测定溶液中蛋白质浓度的新方法。 这种方法简单，易于使用，在蛋白质存在下可以从透明到蓝色容易地看到颜色的变化。

公开(公告)号：US08124727B2

公开(公告)日：2012-02-28

申请号：US12288646

申请日：2008-10-22

申请人： Ashok K. Shukla ,  Mukta M Shukla

发明人： Ashok K. Shukla ,  Mukta M Shukla

IPC分类号： C07K1/14 ,  C07K17/00

CPC分类号： C07K1/16 ,  C07K1/14

摘要： Use of modified metal oxides for the purification and enrichment of negatively charged biomolecules such as peptides, proteins, DNA, RNA, Lipids, carbohydrates, glyco molecules. These metal oxides are modified in such a way that the density of the Lewis acid group is reduced due to modification.

摘要翻译： 使用改性金属氧化物来净化和富集带负电荷的生物分子如肽，蛋白质，DNA，RNA，脂质，碳水化合物，糖分子。 这些金属氧化物以使路易斯酸基的密度由于改性而降低的方式进行了改性。

公开(公告)号：US06537502B1

公开(公告)日：2003-03-25

申请号：US09625559

申请日：2000-07-25

申请人： Ashok K Shukla ,  Amita M Shukla ,  Mukta M Shukla

发明人： Ashok K Shukla ,  Amita M Shukla ,  Mukta M Shukla

IPC分类号： B01L302

CPC分类号： B01J20/103 ,  B01J20/0211 ,  B01J20/20 ,  B01J20/28 ,  B01J20/28004 ,  B01J20/28014 ,  B01J20/28026 ,  B01J20/283 ,  B01J20/286 ,  B01J20/287 ,  B01J20/321 ,  B01J20/3234 ,  B01J20/3246 ,  B01J2220/52 ,  B01J2220/58 ,  B01J2220/64 ,  B01J2220/86 ,  B01L3/0275 ,  B01L2300/0681 ,  G01N30/6004 ,  G01N30/6065 ,  G01N2035/1055 ,  Y10T436/25

摘要： This invention relates to a novel method for small sample preparation using a tube or column, such as a pipette tip, in which the interior surface is coated with a solid matrix for sample preparation. Said solid matrix is composed of a polymeric substance such as polytetrafluoroethylene (PTFE) and one or more column materials such as reactive or absorptive materials suited for sample filtration, separation or purification. The desired sample, containing bio-molecules such as DNA, proteins or other molecular components, is passed through said tube or column, which may be a pipette tip, or like structure.

摘要翻译： 本发明涉及一种使用诸如移液管尖端的管或柱的小样品制备的新方法，其中内表面涂覆有用于样品制备的固体基质。 所述固体基质由聚四氟乙烯（PTFE）和一种或多种柱材料如聚合物质组成，例如适用于样品过滤，分离或纯化的反应性或吸收性材料。 包含生物分子如DNA，蛋白质或其他分子组分的所需样品通过所述管或柱，其可以是移液管尖端或类似结构。

公开(公告)号：US06320030B1

公开(公告)日：2001-11-20

申请号：US09696897

申请日：2000-10-26

申请人： Ashok K Shukla ,  Mukta M Shukla ,  Amita M Shukla

发明人： Ashok K Shukla ,  Mukta M Shukla ,  Amita M Shukla

IPC分类号： C12N1500

CPC分类号： C12N15/87 ,  A61K39/39 ,  A61K47/42 ,  A61K47/61 ,  A61K2039/55516 ,  C07K14/4727

摘要： In the present invention we describe a new method for the formation of a mucin-biomolecules complex, such as a mucin-DNA (deoxyribonucleic acid) complex and the application of such a complex for the transport of DNA, RNA (ribonucleic acid) and other biomolecules into cells. Transfection is the introduction of a DNA molecule into a eukaryotic cell, usually followed by the expression of one or more genes in the newly introduced DNA. The mucin-DNA complex described in the present invention can be used to perform transfection of DNA, as well as, the introduction of RNA and other larger biomolecules into cells. Since effective transfection, especially in in vivo systems is still limited by the methods currently available, the mucin-DNA complex, as described in the present invention, presents a novel and significantly improved method for performing transfection and ensuring the effective transmission of DNA into cells and the expression of genes in transfected DNA.

摘要翻译： 在本发明中，我们描述了一种用于形成粘蛋白生物分子复合物的新方法，例如粘蛋白-DNA（脱氧核糖核酸）复合物，以及这种复合物在DNA，RNA（核糖核酸）等转运中的应用 生物分子进入细胞。 转染是将DNA分子引入真核细胞，通常在新引入的DNA中通常表达一个或多个基因。 本发明中描述的粘蛋白-DNA复合物可用于进行DNA的转染，以及将RNA和其它更大的生物分子引入细胞中。 因为有效的转染，特别是在体内系统中，仍受限于目前可获得的方法，本发明所述的粘蛋白-DNA复合物提供了一种新颖且显着改进的方法，用于进行转染并确保DNA有效传递到细胞中 和转染DNA中基因的表达。

公开(公告)号：US6028525A

公开(公告)日：2000-02-22

申请号：US33692

申请日：1998-03-03

申请人： Ashok K Shukla ,  Mukta M Shukla

发明人： Ashok K Shukla ,  Mukta M Shukla

IPC分类号： G01N30/88 ,  G08B21/00

CPC分类号： G01N30/88 ,  G01N2030/8804

摘要： This invention relates to a tilt switch, which when activated sends signals to a wireless remote receiver. The receiver will alert the human operator and/or execute a process or any programmed action.

摘要翻译： 本发明涉及一种倾斜开关，其在被激活时向无线远程接收器发送信号。 接收器将提醒人员和/或执行过程或任何编程的动作。

公开(公告)号：US5773238A

公开(公告)日：1998-06-30

申请号：US499667

申请日：1995-07-07

申请人： Ashok K. Shukla

发明人： Ashok K. Shukla

IPC分类号： C12N5/00 ,  C12P1/00 ,  C12N11/04 ,  C12N13/00

CPC分类号： B01L3/502792 ,  B01L3/5088 ,  C12N5/0068 ,  B01F13/0071 ,  B01L2200/16 ,  B01L2300/165 ,  C12N2533/30

摘要： A reaction chamber is constructed of a reactant-containing aqueous solution, which may be in droplet form, coated with a fluoropolymer powder such as Polytetrafluoroethylene (PTFE), preferably having a particle size of less than 500 microns. After a reaction, the droplet is destroyed by adding a substance such as a detergent or organic solvent, and the fluoropolymer powder is removed by centrifuging and filtering. Using a micropipette, size of the droplet chamber is increased or decreased by removing or adding liquid, or liquid is transferred from one droplet chamber to another. Charcoal, metal powder or silica powder can be inserted inside or on the surface of the droplet. A droplet chamber containing a first reactant such as an enzyme-bound bead is combined with a second droplet chamber containing a second reactant to react the first and second reactants. A dialysis chamber contains a filtration membrane in contact with the droplet chamber. Placing the droplet chamber between two electrodes and producing current flow between the electrodes provides an electrochemical reaction. A temperature-sensitive reaction such as the Polymerase Chain Reaction (PCR) is performed by heating or cooling the droplet chamber. The droplet reaction chamber is advantageous for microvolumes of sample since container walls are not present on which the sample can disperse, and the chamber does not require significant energy for heating or cooling. In tissue culture, coating a solution containing tissue cells with the fluoropolymer powder reduces sticking of the cells to surfaces of a container.

摘要翻译： 反应室由可以是液滴形式的含反应物的水溶液构成，涂覆有氟聚合物粉末，例如聚四氟乙烯（PTFE），优选具有小于500微米的粒度。 反应后，通过添加洗涤剂或有机溶剂等物质来破坏液滴，通过离心分离除去含氟聚合物粉末。 使用微量移液管，通过去除或添加液体来增加或减少液滴室的尺寸，或者液体从一个液滴室转移到另一液滴室。 木炭，金属粉末或二氧化硅粉末可以插入液滴的内部或表面。 含有第一反应物如酶结合的珠的液滴室与含有第二反应物的第二液滴室结合以使第一和第二反应物反应。 透析室包含与液滴室接触的过滤膜。 将液滴室放置在两个电极之间并在电极之间产生电流提供电化学反应。 通过加热或冷却液滴室来进行诸如聚合酶链式反应（PCR）的温度敏感反应。 液滴反应室对于样品的微量体积是有利的，因为不存在样品可以分散的容器壁，并且室不需要显着的能量用于加热或冷却。 在组织培养中，用含氟聚合物粉末涂布含有组织细胞的溶液可以减少细胞对容器表面的粘附。

公开(公告)号：US5745918A

公开(公告)日：1998-05-05

申请号：US853478

申请日：1997-05-09

申请人： Mukta M. Shukla ,  Ashok K. Shukla

发明人： Mukta M. Shukla ,  Ashok K. Shukla

IPC分类号： A41D25/00 ,  A41D27/08

CPC分类号： A41D25/00 ,  A41D27/08

摘要： This invention relates to the use of the conventional necktie as a source of information or for communicating a message in text and/or graphic, either by printing, painting, embroidering, or by thermal, chemical, and physical bonding to the surface of the necktie.

摘要翻译： 本发明涉及常规领带作为信息来源或通过印刷，绘画，刺绣或通过热，化学和物理粘合到领带的表面来传递文本和/或图形中的消息的用途 。

公开(公告)号：US5733442A

公开(公告)日：1998-03-31

申请号：US225667

申请日：1994-04-11

申请人： Ashok K. Shukla

发明人： Ashok K. Shukla

IPC分类号： B01D57/02 ,  B01D61/28 ,  B01D61/42 ,  B01F13/08 ,  C12N15/10 ,  G01N1/34 ,  G01N27/447 ,  B01D63/00

CPC分类号： B01D57/02 ,  B01D61/28 ,  B01D61/422 ,  B01D61/425 ,  B01F13/0818 ,  C12N15/101 ,  G01N27/4473 ,  G01N2001/4016

摘要： A microdialysis system for the dialysis of small sample volumes of protein, nucleic acid, peptide, and other biomolecules has been developed. The device may contain a built-in magnet to permit remote rotation of the device during dialysis. Double sided dialysis chambers can be used for electrodialysis, electroelution, or electroconcentration. Two or more chambers can be joined either by a union or directly for equilibrium dialysis, or other applications.

摘要翻译： 已经开发了用于透析小样品体积的蛋白质，核酸，肽和其他生物分子的微透析系统。 该装置可以包含内置的磁体，以允许透析期间设备的远程旋转。 双面透析室可用于电渗析，电洗脱或电浓缩。 两个或更多个室可以通过联合或直接连接用于平衡透析或其它应用。